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![Immunoprecipitation:KAT4/TBP Associated Factor 1 AntibodyNBP3-29706] -Detection of human TAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAF1 antibody NBP3-29706 (lot NBP3-29706-2) used for IP at 6 µg per reaction. TAF1 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29706-1) and rabbit anti-TAF1.. For blotting immunoprecipitated TAF1, NBP3-29706 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp3-29706_rabbit-kat4-tbp-associated-factor-1-pab-301220241627347.jpg "Immunoprecipitation:KAT4/TBP Associated Factor 1 AntibodyNBP3-29706] -Detection of human TAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAF1 antibody NBP3-29706 (lot NBP3-29706-2) used for IP at 6 µg per reaction. TAF1 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29706-1) and rabbit anti-TAF1.. For blotting immunoprecipitated TAF1, NBP3-29706 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Immunoprecipitation:SRM AntibodyNBP3-30219] -Detection of human SRM by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRM antibody NBP3-30219 (lot NBP3-30219-1) used for IP at 6 µg per reaction. SRM was also immunoprecipitated by rabbit anti-SRM.. For blotting immunoprecipitated SRM, NBP3-30219 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30219_rabbit-srm-pab-30122024124400.jpg "Immunoprecipitation:SRM AntibodyNBP3-30219] -Detection of human SRM by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRM antibody NBP3-30219 (lot NBP3-30219-1) used for IP at 6 µg per reaction. SRM was also immunoprecipitated by rabbit anti-SRM.. For blotting immunoprecipitated SRM, NBP3-30219 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:PTK7/CCK4 AntibodyNBP3-30221] -Detection of human PTK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PTK7 antibody NBP3-30221 (lot NBP3-30221-1) used for IP at 6 µg per reaction. PTK7 was also immunoprecipitated by rabbit anti-PTK7.. For blotting immunoprecipitated PTK7, NBP3-30221 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30221_rabbit-ptk7-cck4-pab-301220242046548.jpg "Immunoprecipitation:PTK7/CCK4 AntibodyNBP3-30221] -Detection of human PTK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PTK7 antibody NBP3-30221 (lot NBP3-30221-1) used for IP at 6 µg per reaction. PTK7 was also immunoprecipitated by rabbit anti-PTK7.. For blotting immunoprecipitated PTK7, NBP3-30221 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ERK2-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18632-img0001.jpg "Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.")
![Immunoprecipitation:PTK7/CCK4 AntibodyNBP3-29135] -Detection of human PTK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PTK7 antibody NBP3-29135 (lot NBP3-29135-1) used for IP at 6 µg per reaction. PTK7 was also immunoprecipitated by rabbit anti-PTK7.. For blotting immunoprecipitated PTK7, NBP3-29135 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29135_rabbit-ptk7-cck4-pab-3012202420334519.jpg "Immunoprecipitation:PTK7/CCK4 AntibodyNBP3-29135] -Detection of human PTK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PTK7 antibody NBP3-29135 (lot NBP3-29135-1) used for IP at 6 µg per reaction. PTK7 was also immunoprecipitated by rabbit anti-PTK7.. For blotting immunoprecipitated PTK7, NBP3-29135 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:SRM AntibodyNBP3-29194] -Detection of human SRM by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRM antibody NBP3-29194 (lot NBP3-29194-1) used for IP at 6 µg per reaction. SRM was also immunoprecipitated by rabbit anti-SRM.. For blotting immunoprecipitated SRM, NBP3-29194 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29194_rabbit-srm-pab-3012202412392139.jpg "Immunoprecipitation:SRM AntibodyNBP3-29194] -Detection of human SRM by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRM antibody NBP3-29194 (lot NBP3-29194-1) used for IP at 6 µg per reaction. SRM was also immunoprecipitated by rabbit anti-SRM.. For blotting immunoprecipitated SRM, NBP3-29194 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: TNIK Antibody (HL1751) - Azide and BSA Free [NBP3-25725] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TNIK antibody [HL1751] (NBP3-25725) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25725_rabbit-tnik-mab-hl1751-azide-and-bsa-free-2120249342716.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TNIK Antibody (HL1751) - Azide and BSA Free [NBP3-25725] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TNIK antibody [HL1751] (NBP3-25725) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: TNIK Antibody (HL1751) - Azide and BSA Free [NBP3-25725] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with TNIK antibody [HL1751] (NBP3-25725) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25725_rabbit-tnik-mab-hl1751-azide-and-bsa-free-2120249432867.jpg)
![Western Blot:[NBP3-29585]-Detection of human and mouse STK33 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-STK33 antibody used for WB at 0. 04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29585_rabbit-stk33-pab-23120251252873.jpg "Western Blot:[NBP3-29585]-Detection of human and mouse STK33 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-STK33 antibody used for WB at 0. 04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.")
![<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - ErbB2 (phospho Tyr1139) antibody detects ErbB2 (phospho Tyr1139) protein by western blot analysis. Un-treated (-) and treated (+, 200ng/ml Trichostatin A treatment for 15min) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2 (phospho Tyr1139) antibody (NBP3-12918) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0001.jpg)
![Western Blot:[NBP3-29010]-Detection of human and mouse SCYL2 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCYL2 antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29010_rabbit-scyl2-pab-231202511595063.jpg "Western Blot:[NBP3-29010]-Detection of human and mouse SCYL2 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCYL2 antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Ser380]-Antibody-Western-Blot-NBP3-13243-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Ser380]-Antibody-Western-Blot-NBP3-13243-img0001.jpg)