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![Western Blot: PKC theta [p Thr219] Antibody (HL1157) - Azide and BSA Free [NBP3-25607] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Thr219) antibody [HL1157] (NBP3-25607) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25607_rabbit-pkc-theta-p-thr-219-mab-hl1157-azide-bsa-free-2120249362744.jpg "<b>Biological Strategies Validation. </b>Western Blot: PKC theta [p Thr219] Antibody (HL1157) - Azide and BSA Free [NBP3-25607] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Thr219) antibody [HL1157] (NBP3-25607) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta antibody [HL1157] (NBP3-25607) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25607_rabbit-pkc-theta-p-thr-219-mab-hl1157-azide-bsa-free-western-blot-141120251842414.jpg)