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![Immunohistochemistry-Paraffin: PDGF R beta Antibody [NBP3-11723] - PDGF R beta antibody IHC of human kidney, renal tubular epithelium in cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PDGF-R-beta-Antibody-Immunohistochemistry-Paraffin-NBP3-11723-img0001.jpg "Immunohistochemistry-Paraffin: PDGF R beta Antibody [NBP3-11723] - PDGF R beta antibody IHC of human kidney, renal tubular epithelium in cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: PIM1 Antibody [NBP3-14446] - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/PIM1 Antibody-Immunohistochemistry-Paraffin-NBP3-14446-img0001.jpg "Immunohistochemistry-Paraffin: PIM1 Antibody [NBP3-14446] - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Western Blot: PIM1 Antibody [NBP3-12778] - Lane A: Jurkat Whole Cell LysateLane B: U937 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:32 kDa](http://images.novusbio.com/fullsize/PIM1-Antibody-Western-Blot-NBP3-12778-img0001.jpg "Western Blot: PIM1 Antibody [NBP3-12778] - Lane A: Jurkat Whole Cell LysateLane B: U937 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:32 kDa")
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (7N1S0) [NBP3-16960] - Western blot analysis of extracts of A-431 cells, using ErbB2/Her2 (NBP3-16960) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ErbB2-Her2-p-Tyr1139-Antibody-7N1S0-Western-Blot-NBP3-16960-img0001.jpg "Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (7N1S0) [NBP3-16960] - Western blot analysis of extracts of A-431 cells, using ErbB2/Her2 (NBP3-16960) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Flow Cytometry: ErbB2/Her2 Antibody (24D2) [NBP3-09136] - Separation of MCF-7 cells stained using anti-human CD340 (24D2) purified antibody (concentration in sample 5,0 ug/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-24D2-Flow-Cytometry-NBP3-09136-img0001.jpg "Flow Cytometry: ErbB2/Her2 Antibody (24D2) [NBP3-09136] - Separation of MCF-7 cells stained using anti-human CD340 (24D2) purified antibody (concentration in sample 5,0 ug/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).")
![Immunohistochemistry: HIST1H3A Antibody (35C11) - K27M mutant [NBP3-26125] - Image of HIST1H3A Antibody (35C11) - K27M mutant diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.08% DAB.](http://images.novusbio.com/fullsize/nbp3-26125_rabbit-hist1h3a-mab-35c11-k27m-mutant-2722024856316.jpg "Immunohistochemistry: HIST1H3A Antibody (35C11) - K27M mutant [NBP3-26125] - Image of HIST1H3A Antibody (35C11) - K27M mutant diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.08% DAB.")
![Western Blot: HGFR/c-MET [p Tyr1349] Antibody (S05-3D6) [NBP3-14874] - Western blot detection of HGFR/c-MET [p Tyr1349] in Hela, A549 cell lysates using HGFR/c-MET [p Tyr1349] Rabbit mAb (1:1000 diluted). Predicted band size: 156kDa. Observed band size: 170-140kDa.](http://images.novusbio.com/fullsize/HGFR-c-MET-p-Tyr1349-Antibody-S05-3D6-Western-Blot-NBP3-14874-img0001.jpg "Western Blot: HGFR/c-MET [p Tyr1349] Antibody (S05-3D6) [NBP3-14874] - Western blot detection of HGFR/c-MET [p Tyr1349] in Hela, A549 cell lysates using HGFR/c-MET [p Tyr1349] Rabbit mAb (1:1000 diluted). Predicted band size: 156kDa. Observed band size: 170-140kDa.")
![Western Blot: ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12) [NBP3-32769] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32769) at 1/1,000 dilution. Lane 1: SK-Br-3 whole cell lysate Lane 2: SK-Br-3 treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32769) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32769_rabbit-erbb2-her2-p-tyr1221-p-tyr1222-mab-je44-12-288202412442970.jpg "Western Blot: ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12) [NBP3-32769] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32769) at 1/1,000 dilution. Lane 1: SK-Br-3 whole cell lysate Lane 2: SK-Br-3 treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32769) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")