| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26386-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34753-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26462-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45004 | |||
| Azide and BSA Free | NBP2-34801-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84409-25ul | |||
![Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Positive Western Blot detected in 293 whole cell lysate (treated with Calyculin A or Pervanadate). All lanes: p53 [p Thr55] Antibody at 1.28ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 KDa Observed band size: 53 KDa](http://images.novusbio.com/fullsize/nbp3-26386_rabbit-p53-p-thr-55-mab-3c3-282202411351620.jpg "Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Positive Western Blot detected in 293 whole cell lysate (treated with Calyculin A or Pervanadate). All lanes: p53 [p Thr55] Antibody at 1.28ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 KDa Observed band size: 53 KDa")
![Immunocytochemistry/Immunofluorescence: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Staining of 293 cells (treated with 50mM Calyculin A for 30min) with p53 [p Thr55] Antibody (3C3) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26386_rabbit-p53-p-thr-55-mab-3c3-282202412493626.jpg)
![Western Blot: GRAF Antibody [NBP2-32229] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GRAF antibody NBP2-32229 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GRAF-Antibody-Western-Blot-NBP2-32229-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: GRAF Antibody [NBP2-32229] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GRAF antibody NBP2-32229 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: GRAF Antibody [NBP2-32229] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-GRAF antibody NBP2-32229 used for IP at 6 ug per reaction. For blotting immunoprecipitated GRAF, NBP2-32229 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GRAF-Antibody-Immunoprecipitation-NBP2-32229-img0002.jpg)
![Immunohistochemistry: Caspase-3 Antibody (3H12) - Active, Pro [NBP3-26462] - Image of Caspase-3 Antibody (3H12) - Active, Pro diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26462_rabbit-caspase-3-mab-3h12-active-pro-262202414471570.jpg "Immunohistochemistry: Caspase-3 Antibody (3H12) - Active, Pro [NBP3-26462] - Image of Caspase-3 Antibody (3H12) - Active, Pro diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Caspase-3 Antibody (3H12) - Active, Pro [NBP3-26462] - Positive Western Blot detected in: Hela whole cell lysate, HEK293 whole cell lysate, Ntera-2 cell lysate. All lanes: Caspase-3 Antibody - Active, Pro at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32, 55, 130 kDa](http://images.novusbio.com/fullsize/nbp3-26462_rabbit-caspase-3-mab-3h12-active-pro-28220241147218.jpg)
![Western Blot: PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12) [NBP3-15222] - Positive WB detected in A549 whole cell lysate (treated with EGF or not). All lanes: PAK1/2/3 antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 65 KDa. Observed band size: 65 KDa.](http://images.novusbio.com/fullsize/PAK1-2-3-p-Ser144-p-Ser141-p-Ser139-Antibody-3H12-Western-Blot-NBP3-15222-img0002.jpg "Western Blot: PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12) [NBP3-15222] - Positive WB detected in A549 whole cell lysate (treated with EGF or not). All lanes: PAK1/2/3 antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 65 KDa. Observed band size: 65 KDa.")
![Immunohistochemistry: PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12) [NBP3-15222] - IHC image of PAK1/2/3 antibody diluted at 1:100 and staining in paraffin-embedded rat brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/PAK1-2-3-p-Ser144-p-Ser141-p-Ser139-Antibody-3H12-Immunohistochemistry-NBP3-15222-img0001.jpg)
![Western Blot: Serpin E2/PN1 Antibody [NBP2-20314] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-Western-Blot-NBP2-20314-img0002.jpg "Western Blot: Serpin E2/PN1 Antibody [NBP2-20314] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 10% SDS PAGE gel, diluted at 1:500.")
![Western Blot: Serpin E2/PN1 Antibody [NBP2-20314] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Serpin E2/PN1 antibody (NBP2-20314) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20314_rabbit-polyclonal-serpin-e2-pn1-antibody-910202420115045.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (SPM567) [NBP2-32965] - Western blot analysis of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor Ab (SPM567).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-SPM567-Western-Blot-NBP2-32965-img0002.jpg "Western Blot: ER alpha/NR3A1 Antibody (SPM567) [NBP2-32965] - Western blot analysis of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor Ab (SPM567).")
![Immunohistochemistry-Paraffin: SEMA3A Antibody [NBP1-84409] - Staining of human liver shows cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SEMA3A-Antibody-Immunohistochemistry-Paraffin-NBP1-84409-img0002.jpg "Immunohistochemistry-Paraffin: SEMA3A Antibody [NBP1-84409] - Staining of human liver shows cytoplasmic positivity in hepatocytes.")