| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61769-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-97515-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89197-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97750-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98752-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00194-50ul | |||
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0003.jpg "Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)")
![Flow Cytometry: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Flow-Cytometry-NBP2-61769-img0002.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0004.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-ELISA-NBP2-61769-img0001.jpg)
![Western Blot: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521] - Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-OTI3H8-Azide-and-BSA-Free-Western-Blot-NBP2-72521-img0001.jpg "Western Blot: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521] - Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.")
![Immunohistochemistry: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-OTI3H8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72521-img0002.jpg)
![Western Blot: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71).](http://images.novusbio.com/fullsize/Vimentin-p-Ser71-Antibody-TM71-Western-Blot-NBP1-97515-img0001.jpg "Western Blot: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71).")
![Immunocytochemistry/Immunofluorescence: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) on 4% PFA fixed U251 cells with [pSer71]Vimentin, mAb (TM71).](http://images.novusbio.com/fullsize/Vimentin-p-Ser71-Antibody-TM71-Immunohistochemistry-NBP1-97515-img0002.jpg)
![Western Blot: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Western blot analysis of Glutamine Synthetase on different lysates with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: HEK-293 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32387) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32387_mouse-glutamine-synthetase-mab-a3g2-r-278202415242686.jpg "Western Blot: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Western blot analysis of Glutamine Synthetase on different lysates with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: HEK-293 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32387) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32387) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32387_mouse-glutamine-synthetase-mab-a3g2-r-298202414333698.jpg)
![Western Blot: ALDH1A2 Antibody (JE41-38) [NBP3-32020] - Western blot analysis of ALDH1A2 on different lysates with Rabbit anti-ALDH1A2 antibody (NBP3-32020) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 57 kDa Observed band size: 53 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32020) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32020_rabbit-aldh1a2-mab-je41-38-278202415141711.jpg "Western Blot: ALDH1A2 Antibody (JE41-38) [NBP3-32020] - Western blot analysis of ALDH1A2 on different lysates with Rabbit anti-ALDH1A2 antibody (NBP3-32020) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 57 kDa Observed band size: 53 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32020) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ALDH1A2 Antibody (JE41-38) [NBP3-32020] - Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-ALDH1A2 antibody (NBP3-32020) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32020) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32020_rabbit-aldh1a2-mab-je41-38-29820241435289.jpg)
![Western Blot: DDR2 Antibody (JE31-45) [NBP3-32260] - Western blot analysis of DDR2 on different lysates with Rabbit anti-DDR2 antibody (NBP3-32260) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: SH-SY5Y cell lysate (40 ug/Lane) Lane 3: L929 cell lysate (40 ug/Lane) Lane 4: NIH/3T3 cell lysate (40 ug/Lane) Lane 5: U-2 OS cell lysate (15 ug/Lane) Lane 6: A375 cell lysate (15 ug/Lane) Lane 7: PC-12 cell lysate (10 ug/Lane) Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32260) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32260_rabbit-ddr2-mab-je31-45-27820241631680.jpg "Western Blot: DDR2 Antibody (JE31-45) [NBP3-32260] - Western blot analysis of DDR2 on different lysates with Rabbit anti-DDR2 antibody (NBP3-32260) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: SH-SY5Y cell lysate (40 ug/Lane) Lane 3: L929 cell lysate (40 ug/Lane) Lane 4: NIH/3T3 cell lysate (40 ug/Lane) Lane 5: U-2 OS cell lysate (15 ug/Lane) Lane 6: A375 cell lysate (15 ug/Lane) Lane 7: PC-12 cell lysate (10 ug/Lane) Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32260) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DDR2 Antibody (JE31-45) [NBP3-32260] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-DDR2 antibody (NBP3-32260) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32260) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32260_rabbit-ddr2-mab-je31-45-298202414161855.jpg)
![Western Blot: MAP2 Antibody (MT-01) [NB500-415] - Fig. 1. Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01) Lane 2: immunostaining with anti-MAP2ab (MT-07) Lane 3: immunostaining with anti-MAP2ab (MT-08). Observed molecular weight is approximately 270 kDA.](http://images.novusbio.com/fullsize/MAP2-Antibody-MT-01-Western-Blot-NB500-415-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MAP2 Antibody (MT-01) [NB500-415] - Fig. 1. Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01) Lane 2: immunostaining with anti-MAP2ab (MT-07) Lane 3: immunostaining with anti-MAP2ab (MT-08). Observed molecular weight is approximately 270 kDA.")
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human brain with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-042-Immunohistochemistry-Paraffin-NBP2-89197-img0003.jpg "Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human brain with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).")
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human placenta with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-042-Immunohistochemistry-Paraffin-NBP2-89197-img0001.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human heart with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-042-Immunohistochemistry-Paraffin-NBP2-89197-img0002.jpg)
![Western Blot: CRMP2 Antibody [NBP2-97750] - Anti-CRMP2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U87MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa](http://images.novusbio.com/fullsize/CRMP2-Antibody-Western-Blot-NBP2-97750-img0004.jpg "Western Blot: CRMP2 Antibody [NBP2-97750] - Anti-CRMP2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U87MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa")
![Immunoprecipitation: CRMP2 Antibody [NBP2-97750] - CRMP2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-CRMP2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CRMP2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa](http://images.novusbio.com/fullsize/CRMP2-Antibody-In-ovo-assay-NBP2-97750-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CRMP2 Antibody [NBP2-97750] - Immunofluorescence staining of CRMP2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97750 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97750_rabbit-polyclonal-crmp2-antibody-23520251129349.png)
![Western Blot: Stathmin 1 Antibody [NBP2-98752] - Anti-Stathmin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP2-98752-img0001.jpg "Western Blot: Stathmin 1 Antibody [NBP2-98752] - Anti-Stathmin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa")
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP2-98752] - Immunochemical staining of human Stathmin 1 in human tonsil with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98752-img0003.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP2-98752] - Immunochemical staining of human Stathmin 1 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98752-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Calsequestrin 1 Antibody [NBP3-00194] - Immunofluorescence staining of Calsequestrin 1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00194 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-00194_rabbit-polyclonal-calsequestrin-1-antibody-23520251664755.png "Immunocytochemistry/ Immunofluorescence: Calsequestrin 1 Antibody [NBP3-00194] - Immunofluorescence staining of Calsequestrin 1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00194 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP3-00194] - Immunochemical staining of Calsequestrin 1 in human skeletal muscle with NBP3-00194 at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00194_rabbit-polyclonal-calsequestrin-1-antibody-23520252135113.png)
![Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP3-00194] - Immunochemical staining of Calsequestrin 1 in cynomolgus heart with NBP3-00194 at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00194_rabbit-polyclonal-calsequestrin-1-antibody-23520251433156.png)
![Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-278202416331236.jpg "Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32046) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-29820241492659.jpg)