| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61769-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89197-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90271-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90139-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98294-50ul | |||
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0003.jpg "Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)")
![Flow Cytometry: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Flow-Cytometry-NBP2-61769-img0002.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0004.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-ELISA-NBP2-61769-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58367] - Anti-Coronin 1A](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58367-img0003.jpg "Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58367] - Anti-Coronin 1A")
![Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-278202416285257.jpg "Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ATG12 Antibody (JE30-61) [NBP3-32056] - Immunocytochemistry analysis of HCT 116 cells labeling ATG12 with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-2920241192327.jpg)
![Flow Cytometry: ATG12 Antibody (JE30-61) [NBP3-32056] - Flow cytometric analysis of HCT 116 cells labeling ATG12. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32056, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-29202416453286.jpg)
![Western Blot: CRBN Antibody (PSH0-70) [NBP3-32217] - Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (NBP3-32217) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32217) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32217_rabbit-crbn-mab-psh0-70-27820241628525.jpg "Western Blot: CRBN Antibody (PSH0-70) [NBP3-32217] - Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (NBP3-32217) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32217) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CRBN Antibody (PSH0-70) [NBP3-32217] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CRBN antibody (NBP3-32217) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32217) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32217_rabbit-crbn-mab-psh0-70-298202413585562.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human brain with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-042-Immunohistochemistry-Paraffin-NBP2-89197-img0003.jpg "Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human brain with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).")
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human placenta with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-042-Immunohistochemistry-Paraffin-NBP2-89197-img0001.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human heart with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-042-Immunohistochemistry-Paraffin-NBP2-89197-img0002.jpg)
![Western Blot: NFkB p100/p52 Antibody [NBP2-87905] - Host: Rabbit. Target Name: NFKB2. Sample Tissue: Human HCT116. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP2-87905-img0003.jpg "Western Blot: NFkB p100/p52 Antibody [NBP2-87905] - Host: Rabbit. Target Name: NFKB2. Sample Tissue: Human HCT116. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: NFkB p100/p52 Antibody [NBP2-87905] - Human Muscle](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-NBP2-87905-img0002.jpg)
![Immunohistochemistry: NFkB p100/p52 Antibody [NBP2-87905] - Human Smooth Muscle](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-NBP2-87905-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0002.jpg "Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunofluorescence staining of AIF-1/Iba1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90271 (dilution ratio 1:60) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90271_rabbit-monoclonal-aif-1-iba1-antibody-031-235202514492357.png)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human breast-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0004.jpg "Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human breast-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0001.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0002.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human colon-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0003.jpg)
![Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-278202415242680.jpg "Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32458) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-298202414333652.jpg)
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-98294] - Immunochemical staining of mouse CRABP2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98294-img0003.jpg "Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-98294] - Immunochemical staining of mouse CRABP2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-98294] - Immunochemical staining of mouse CRABP2 in mouse kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98294-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CRABP2 Antibody [NBP2-98294] - Immunofluorescence staining of mCRABP2 in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98294 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98294_rabbit-polyclonal-crabp2-antibody-23520251129330.png)
![Western Blot: PSME4 Antibody [NBP3-32869] - Western blot analysis of PSME4 on different lysates with Rabbit anti-PSME4 antibody (NBP3-32869) at 1/500 dilution. Lane 1: 293T cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: Jurkat cell lysate (10 ug/Lane) Lane 4: RAW264.7 cell lysate (10 ug/Lane) Lane 5: Rat skeletal muscle tissue lysate (20 ug/Lane) Predicted band size: 211 kDa Observed band size: 211 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32869) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32869_rabbit-psme4-pab-288202412442999.jpg "Western Blot: PSME4 Antibody [NBP3-32869] - Western blot analysis of PSME4 on different lysates with Rabbit anti-PSME4 antibody (NBP3-32869) at 1/500 dilution. Lane 1: 293T cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: Jurkat cell lysate (10 ug/Lane) Lane 4: RAW264.7 cell lysate (10 ug/Lane) Lane 5: Rat skeletal muscle tissue lysate (20 ug/Lane) Predicted band size: 211 kDa Observed band size: 211 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32869) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PSME4 Antibody [NBP3-32869] - Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PSME4 antibody (NBP3-32869) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32869) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32869_rabbit-psme4-pab-298202414262659.jpg)
![Western Blot: Caspr2 Antibody (JE41-03) [NBP3-32110] - Western blot analysis of Caspr2 on different lysates with Rabbit anti-Caspr2 antibody (NBP3-32110) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 148 kDa Observed band size: 150 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32110_rabbit-caspr2-mab-je41-03-27820241524260.jpg "Western Blot: Caspr2 Antibody (JE41-03) [NBP3-32110] - Western blot analysis of Caspr2 on different lysates with Rabbit anti-Caspr2 antibody (NBP3-32110) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 148 kDa Observed band size: 150 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Caspr2 Antibody (JE41-03) [NBP3-32110] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Caspr2 antibody (NBP3-32110) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32110) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32110_rabbit-caspr2-mab-je41-03-29820241435288.jpg)