| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98752-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00194-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90700-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97594-50ul | |||
![Western Blot: NFkB p100/p52 Antibody [NBP2-87905] - Host: Rabbit. Target Name: NFKB2. Sample Tissue: Human HCT116. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP2-87905-img0003.jpg "Western Blot: NFkB p100/p52 Antibody [NBP2-87905] - Host: Rabbit. Target Name: NFKB2. Sample Tissue: Human HCT116. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: NFkB p100/p52 Antibody [NBP2-87905] - Human Muscle](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-NBP2-87905-img0002.jpg)
![Immunohistochemistry: NFkB p100/p52 Antibody [NBP2-87905] - Human Smooth Muscle](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-NBP2-87905-img0001.jpg)
![Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-288202412461623.jpg "Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32808) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32808) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-298202414262664.jpg)
![Western Blot: Stathmin 1 Antibody [NBP2-98752] - Anti-Stathmin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP2-98752-img0001.jpg "Western Blot: Stathmin 1 Antibody [NBP2-98752] - Anti-Stathmin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa")
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP2-98752] - Immunochemical staining of human Stathmin 1 in human tonsil with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98752-img0003.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP2-98752] - Immunochemical staining of human Stathmin 1 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98752-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Calsequestrin 1 Antibody [NBP3-00194] - Immunofluorescence staining of Calsequestrin 1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00194 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-00194_rabbit-polyclonal-calsequestrin-1-antibody-23520251664755.png "Immunocytochemistry/ Immunofluorescence: Calsequestrin 1 Antibody [NBP3-00194] - Immunofluorescence staining of Calsequestrin 1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00194 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP3-00194] - Immunochemical staining of Calsequestrin 1 in human skeletal muscle with NBP3-00194 at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00194_rabbit-polyclonal-calsequestrin-1-antibody-23520252135113.png)
![Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP3-00194] - Immunochemical staining of Calsequestrin 1 in cynomolgus heart with NBP3-00194 at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00194_rabbit-polyclonal-calsequestrin-1-antibody-23520251433156.png)
![Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-288202412461654.jpg "Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32809) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-29820241492670.jpg)
![Immunohistochemistry-Paraffin: Syndecan-4 Antibody (047) [NBP2-90700] - Staining of mouse SDC4 in mouse intestine with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-047-Immunohistochemistry-Paraffin-NBP2-90700-img0003.jpg "Immunohistochemistry-Paraffin: Syndecan-4 Antibody (047) [NBP2-90700] - Staining of mouse SDC4 in mouse intestine with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Syndecan-4 Antibody (047) [NBP2-90700] - Staining of mouse SDC4 in mouse stomach with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-047-Immunohistochemistry-Paraffin-NBP2-90700-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Syndecan-4 Antibody (047) [NBP2-90700] - Immunofluorescence staining of Syndecan-4 in mouse splenocytes. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90700 (1:60) at 4 overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90700_rabbit-monoclonal-syndecan-4-antibody-047-235202514331535.png)
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)
![Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-288202412461622.jpg "Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (JE39-00) [NBP3-32833] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32833) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-298202414304140.jpg)
![Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-28820241419157.jpg "Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-UBA3 antibody (NBP3-33074) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33074) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-298202414262662.jpg)
![Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32283_mouse-dynamin-mab-a1b2-r-27820241631653.jpg "Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dynamin Antibody (A1B2-R) [NBP3-32283] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32283) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32283_mouse-dynamin-mab-a1b2-r-298202414304193.jpg)
![Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-278202415212411.jpg "Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg)
![Immunohistochemistry-Paraffin: COX3 Antibody [NBP2-97594] - staining of human COX3 in human skin with rabbit polyclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/COX3-Antibody-Immunohistochemistry-Paraffin-NBP2-97594-img0003.jpg "Immunohistochemistry-Paraffin: COX3 Antibody [NBP2-97594] - staining of human COX3 in human skin with rabbit polyclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: COX3 Antibody [NBP2-97594] - Staining of human COX3 in human placenta with rabbit polyclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/COX3-Antibody-Immunohistochemistry-Paraffin-NBP2-97594-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: COX3 Antibody [NBP2-97594] - Immunofluorescence staining of COX3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97594 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97594_rabbit-polyclonal-cox3-antibody-23520251628182.png)