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![Western Blot: Slit1 Antibody [NBP2-84269] - WB Suggested Anti-SLIT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Stomach](http://images.novusbio.com/fullsize/Slit1-Antibody-Western-Blot-NBP2-84269-img0002.jpg "Western Blot: Slit1 Antibody [NBP2-84269] - WB Suggested Anti-SLIT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Stomach")
![Immunohistochemistry: Slit1 Antibody [NBP2-84269] - Immunohistochemistry with SLIT1 GFP/ WT mouse gut tissue at an antibody concentration of 2.5 ug/ml using anti-SLIT1 antibody](http://images.novusbio.com/fullsize/Slit1-Antibody-Immunohistochemistry-NBP2-84269-img0001.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP2-87918] - Host: Rabbit. Target Name: CHRNA3. Sample Tissue: Rat Skeletal Muscle. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-Western-Blot-NBP2-87918-img0002.jpg "Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP2-87918] - Host: Rabbit. Target Name: CHRNA3. Sample Tissue: Rat Skeletal Muscle. Antibody Dilution: 1ug/ml")
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP2-87918] - WB Suggested Anti-CHRNA3 Antibody Titration: 0.1ug/ml. Positive Control: Human Thymus](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-Western-Blot-NBP2-87918-img0001.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP2-87918] - Host: Rat. Target Name: CHRNA3. Sample Tissue: Rat Skeletal Muscle. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-Western-Blot-NBP2-87918-img0003.jpg)
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Immunohistochemistry-Paraffin: mu Opioid R/OPRM1 Antibody [NLS234] - Brain, Neurons](http://images.novusbio.com/fullsize/mu-Opioid-R-OPRM1-Antibody-Immunohistochemistry-Paraffin-NLS234-img0001.jpg "Immunohistochemistry-Paraffin: mu Opioid R/OPRM1 Antibody [NLS234] - Brain, Neurons")
![Western Blot: Tau Antibody [NBP3-18238] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.](http://images.novusbio.com/fullsize/Tau Antibody-Western Blot-NBP3-18238-img0002.jpg "Western Blot: Tau Antibody [NBP3-18238] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.")
![Immunohistochemistry: Tau Antibody [NBP3-18238] - Immunohistochemistry analysis using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238). Tissue: Brain Cortex. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238).](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-NBP3-18238-img0001.jpg)
![Western Blot: Tau Antibody [NBP3-18237] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa, 65 kDa.](http://images.novusbio.com/fullsize/Tau Antibody-Western Blot-NBP3-18237-img0002.jpg "Western Blot: Tau Antibody [NBP3-18237] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa, 65 kDa.")
![Immunohistochemistry: Tau Antibody [NBP3-18237] - Immunohistochemistry analysis using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237). Tissue: Kidney. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237).](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-NBP3-18237-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CGRP Antibody (4901) [NBP2-88945] - Immunostaining of CGRP (red) in cryopreserved DRG neurons grown on coverslips. 40xmag. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. Cells were obtained photo was provided courtesy of QBMCellScience.](http://images.novusbio.com/fullsize/CGRP-Antibody-4901-Immunocytochemistry-Immunofluorescence-NBP2-88945-img0001.jpg "Immunocytochemistry/Immunofluorescence: CGRP Antibody (4901) [NBP2-88945] - Immunostaining of CGRP (red) in cryopreserved DRG neurons grown on coverslips. 40xmag. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. Cells were obtained photo was provided courtesy of QBMCellScience.")
![Western Blot: Amisyn Antibody [NBP2-15376] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Amisyn-Antibody-Western-Blot-NBP2-15376-img0002.jpg "Western Blot: Amisyn Antibody [NBP2-15376] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Amisyn Antibody [NBP2-15376] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Amisyn-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15376-img0001.jpg)
![Western Blot: Stomatin Antibody [NBP2-20520] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Stomatin-Antibody-Western-Blot-NBP2-20520-img0001.jpg "Western Blot: Stomatin Antibody [NBP2-20520] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Stomatin Antibody [NBP2-20520] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Stomatin-Antibody-Immunohistochemistry-Paraffin-NBP2-20520-img0002.jpg)
![Western Blot: Begain Antibody [NBP2-15561] - Sample (30 ug of whole cell lysate) A: Raji7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Begain-Antibody-Western-Blot-NBP2-15561-img0001.jpg "Western Blot: Begain Antibody [NBP2-15561] - Sample (30 ug of whole cell lysate) A: Raji7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Begain Antibody [NBP2-15561] - Analysis of paraffin-embedded Colon carcinoma, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Begain-Antibody-Immunohistochemistry-Paraffin-NBP2-15561-img0003.jpg)
![Western Blot: Calpain 3 Antibody [NBP1-31756] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Calpain-3-Antibody-Western-Blot-NBP1-31756-img0003.jpg "Western Blot: Calpain 3 Antibody [NBP1-31756] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Calpain 3 Antibody [NBP1-31756] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Calpain-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31756-img0002.jpg)
![Western Blot: SIN3A Antibody [NB100-2253] - Detection of Human Sin3A by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody NB100-2253 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Western-Blot-NB100-2253-img0002.jpg "Western Blot: SIN3A Antibody [NB100-2253] - Detection of Human Sin3A by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody NB100-2253 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation: SIN3A Antibody [NB100-2253] - Detection of Human and Mouse Sin3A on HeLa whole cell lysate using NB100-2253. Sin3A was also immunoprecipitated using rabbit anti-Sin3A antibodies NB100-2252, NB100-2254 and another Sin3A antibody.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunoprecipitation-NB100-2253-img0001.jpg)