| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59336-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61881-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52471-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61747-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97806-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12563-50ul | |||
![Western Blot: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Western Blot analysis of Rat brain membrane lysate showing detection of GABA-B R1 protein using Mouse Anti-GABA-B R1 Monoclonal Antibody, Clone S93A-49 (NBP2-59336). Primary Antibody: Mouse Anti-GABA-B R1 Monoclonal Antibody (NBP2-59336) at 1:1000.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-S93A-49-Western-Blot-NBP2-59336-img0005.jpg "Western Blot: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Western Blot analysis of Rat brain membrane lysate showing detection of GABA-B R1 protein using Mouse Anti-GABA-B R1 Monoclonal Antibody, Clone S93A-49 (NBP2-59336). Primary Antibody: Mouse Anti-GABA-B R1 Monoclonal Antibody (NBP2-59336) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody, Clone S93A-49 (NBP2-59336). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody (NBP2-59336) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-S93A-49-Immunocytochemistry-Immunofluorescence-NBP2-59336-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-S93A-49-Immunocytochemistry-Immunofluorescence-NBP2-59336-img0002.jpg)
![Western Blot: GABA-A R alpha 2 Antibody [NBP2-36560] - Western blot of mouse brain lysates from wild type (Control) and alpha2-knockout (alpha2-K/O) animals showing specific immunolabeling of the ~51k alpha2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha2-knockout animals.](http://images.novusbio.com/fullsize/GABA-A-R-alpha-2-Antibody-Western-Blot-NBP2-36560-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GABA-A R alpha 2 Antibody [NBP2-36560] - Western blot of mouse brain lysates from wild type (Control) and alpha2-knockout (alpha2-K/O) animals showing specific immunolabeling of the ~51k alpha2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha2-knockout animals.")
![Western Blot: APBA2 Antibody (1C12A4) [NBP2-61881] - Analysis using APBA2 mAb against human APBA2 (AA: 15-158) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/APBA2-Antibody-1C12A4-Western-Blot-NBP2-61881-img0003.jpg "Western Blot: APBA2 Antibody (1C12A4) [NBP2-61881] - Analysis using APBA2 mAb against human APBA2 (AA: 15-158) recombinant protein. (Expected MW is 42 kDa)")
![Immunocytochemistry/Immunofluorescence: APBA2 Antibody (1C12A4) [NBP2-61881] - Analysis of Hela cells using APBA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/APBA2-Antibody-1C12A4-Immunocytochemistry-Immunofluorescence-NBP2-61881-img0002.jpg)
![Western Blot: APBA2 Antibody (1C12A4) [NBP2-61881] - Analysis using APBA2 mAb against HEK293 (1) and APBA2 (AA: 15-158)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/APBA2-Antibody-1C12A4-Western-Blot-NBP2-61881-img0004.jpg)
![ELISA: APBA2 Antibody (1C12A4) [NBP2-61881] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/APBA2-Antibody-1C12A4-ELISA-NBP2-61881-img0001.jpg)
![Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Western-Blot-NBP2-52471-img0003.jpg "Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis of Hela cells using GAD2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Flow-Cytometry-NBP2-52471-img0001.jpg)
![Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against human GAD2 (AA: 1-100) recombinant protein. (Expected MW is 36.3 kDa)](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Western-Blot-NBP2-52471-img0002.jpg)
![ELISA: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-ELISA-NBP2-52471-img0004.jpg)
![Western Blot: NMDAR3B Antibody (2A8E11) [NBP2-61747] - Analysis using NMDAR3B mAb against human GRIN3B (AA: 135-276) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/NMDAR3B-Antibody-2A8E11-Western-Blot-NBP2-61747-img0003.jpg "Western Blot: NMDAR3B Antibody (2A8E11) [NBP2-61747] - Analysis using NMDAR3B mAb against human GRIN3B (AA: 135-276) recombinant protein. (Expected MW is 40.8 kDa)")
![Flow Cytometry: NMDAR3B Antibody (2A8E11) [NBP2-61747] - Analysis of SK-N-SH cells using GRIN3B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NMDAR3B-Antibody-2A8E11-Flow-Cytometry-NBP2-61747-img0005.jpg)
![Western Blot: NMDAR3B Antibody (2A8E11) [NBP2-61747] - Analysis using NMDAR3B mAb against HEK293 (1) and GRIN3B (AA: 135-276)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NMDAR3B-Antibody-2A8E11-Western-Blot-NBP2-61747-img0004.jpg)
![Flow Cytometry: NMDAR3B Antibody (2A8E11) [NBP2-61747] - Analysis of SH-SY5Y cells using NMDAR3B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NMDAR3B-Antibody-2A8E11-Flow-Cytometry-NBP2-61747-img0002.jpg)
![ELISA: NMDAR3B Antibody (2A8E11) [NBP2-61747] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/NMDAR3B-Antibody-2A8E11-ELISA-NBP2-61747-img0001.jpg)
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-61595] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Monomer-Type-1-Protein-In-vitro-assay-NBP2-61595-img0004.jpg "In vitro assay: Recombinant Mouse alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-61595] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.")
![Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-298202414304191.jpg "Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA-B R1 with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32360, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-298202413585541.jpg)
![Western Blot: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate (no heat) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) Lane 5: Rat cerebellum tissue lysate Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-3920248453530.jpg)
![Western Blot: GLRB Antibody [NBP2-97806] - Anti-GLRB rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/GLRB-Antibody-Western-Blot-NBP2-97806-img0004.jpg "Western Blot: GLRB Antibody [NBP2-97806] - Anti-GLRB rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 72 kDa")
![Immunohistochemistry-Paraffin: GLRB Antibody [NBP2-97806] - Immunochemical staining of human GLRB in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GLRB-Antibody-Immunohistochemistry-Paraffin-NBP2-97806-img0003.jpg)
![Immunoprecipitation: GLRB Antibody [NBP2-97806] - GLRB was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-GLRB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-GLRB rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/GLRB-Antibody-Immunoprecipitation-NBP2-97806-img0005.jpg)
![Western Blot: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Western blot analysis of Neurogranin on different lysates with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 8 kDa Observed band size: 15 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32645) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32645_rabbit-neurogranin-mab-psh02-96-278202415242663.jpg "Western Blot: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Western blot analysis of Neurogranin on different lysates with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 8 kDa Observed band size: 15 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32645) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32645) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32645_rabbit-neurogranin-mab-psh02-96-29820241420998.jpg)
![Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Neurogranin with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32645, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32645_rabbit-neurogranin-mab-psh02-96-298202414161816.jpg)
![Western Blot: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Western blot analysis of Glutamate Receptor 4 on different lysates with Rabbit anti-Glutamate Receptor 4 antibody (NBP3-32384) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate (RIPA lysis) Lane 2: Mouse cerebellum tissue lysate (hot lysis) Lane 3: Rat cerebellum tissue lysate (RIPA lysis) Lane 4: Rat cerebellum tissue lysate (hot lysis) Lysates/proteins at 40 ug/Lane. Predicted band size: 101 kDa Observed band size: 110/60 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32384) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32384_rabbit-glutamate-receptor-4-mab-je36-81-278202415242621.jpg "Western Blot: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Western blot analysis of Glutamate Receptor 4 on different lysates with Rabbit anti-Glutamate Receptor 4 antibody (NBP3-32384) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate (RIPA lysis) Lane 2: Mouse cerebellum tissue lysate (hot lysis) Lane 3: Rat cerebellum tissue lysate (RIPA lysis) Lane 4: Rat cerebellum tissue lysate (hot lysis) Lysates/proteins at 40 ug/Lane. Predicted band size: 101 kDa Observed band size: 110/60 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32384) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Flow cytometric analysis of SH-SY5Y cells labeling Glutamate Receptor 4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32384, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32384_rabbit-glutamate-receptor-4-mab-je36-81-392024884649.jpg)
![Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-12563-img0001.jpg "Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-12563] - Staining of human HCN1 in human brain with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-12563-img0003.jpg)
![Immunoprecipitation: HCN1 Antibody [NBP3-12563] - Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-HCN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HCN1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :100 kDa](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunoprecipitation-NBP3-12563-img0002.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)