| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95125-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03693-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37288-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97928-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42175-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61834-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37734-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89210-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05805-50ul | |||
![Western Blot: SMN2 Antibody [NBP2-95125] - Analysis of extracts of various cell lines, using SMN2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/SMN2-Antibody-Western-Blot-NBP2-95125-img0003.jpg "Western Blot: SMN2 Antibody [NBP2-95125] - Analysis of extracts of various cell lines, using SMN2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Western Blot: SMN2 Antibody [NBP2-95125] - Analysis of extracts of various cell lines, using SMN2 . Exposure time: 30s.](http://images.novusbio.com/fullsize/SMN2-Antibody-Western-Blot-NBP2-95125-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: SMN2 Antibody - BSA Free [NBP2-95125] - Immunofluorescence analysis of U2OS cells using SMN2 antibody (A12519) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95125_rabbit-polyclonal-smn2-antibody-5220251931380.jpg)
![Western Blot: STEP Antibody [NBP3-03693] - Western blot analysis of extracts of various cell lines, using STEP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/STEP-Antibody-Western-Blot-NBP3-03693-img0001.jpg "Western Blot: STEP Antibody [NBP3-03693] - Western blot analysis of extracts of various cell lines, using STEP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of Neuro-2a cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03693_rabbit-polyclonal-step-antibody-52202519302959.jpg)
![Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of SH-SY5Y cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03693_rabbit-polyclonal-step-antibody-5220251931349.jpg)
![Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Western-Blot-NBP2-37288-img0003.jpg "Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Flow cytometric analysis of Hela cells using PPP1R1B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Flow-Cytometry-NBP2-37288-img0004.jpg)
![Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against human PPP1R1B (AA: 95-204) recombinant protein. (Expected MW is 38.3 kDa)](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Western-Blot-NBP2-37288-img0002.jpg)
![ELISA: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-ELISA-NBP2-37288-img0001.jpg)
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-61595] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Monomer-Type-1-Protein-In-vitro-assay-NBP2-61595-img0004.jpg "In vitro assay: Recombinant Mouse alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-61595] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.")
![Western Blot: Aquaporin-4 Antibody [NBP1-97928] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-4 protein using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:2000.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP1-97928-img0007.jpg "Western Blot: Aquaporin-4 Antibody [NBP1-97928] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-4 protein using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:2000.")
![Immunohistochemistry: Aquaporin-4 Antibody [NBP1-97928] - Immunohistochemistry analysis using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 m cryostat sections.](http://images.novusbio.com/fullsize/Aquaporin-4 Antibody-Immunohistochemistry-NBP1-97928-img0008.jpg)
![Western Blot: LRRK2 Antibody (S138-6) [NBP2-42175] - Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (NBP2-42175). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (NBP2-42175) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa.](http://images.novusbio.com/fullsize/LRRK2-Antibody-S138-6-Western-Blot-NBP2-42175-img0003.jpg "Western Blot: LRRK2 Antibody (S138-6) [NBP2-42175] - Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (NBP2-42175). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (NBP2-42175) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa.")
![Immunocytochemistry/Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (NBP2-42175). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (NBP2-42175) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRRK2/Dardarin Antibody (D) Composite.](http://images.novusbio.com/fullsize/LRRK2-Antibody-S138-6-Immunocytochemistry-Immunofluorescence-NBP2-42175-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/LRRK2-Antibody-S138-6-Immunocytochemistry-Immunofluorescence-NBP2-42175-img0002.jpg)
![Western Blot: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis using GRM2 mouse mAb against SK-N-SH (1), Jurkat (2), and SW620 (3) cell lysate.](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-Western-Blot-NBP2-61834-img0004.jpg "Western Blot: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis using GRM2 mouse mAb against SK-N-SH (1), Jurkat (2), and SW620 (3) cell lysate.")
![Western Blot: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis using GRM2 mAb against human GRM2 (AA: extra 414-558) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-Western-Blot-NBP2-61834-img0003.jpg)
![Western Blot: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis using GRM2 mAb against HEK293 (1) and GRM2 (AA: extra 414-558)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-Western-Blot-NBP2-61834-img0005.jpg)
![ELISA: mGluR2 Antibody (6E4F6) [NBP2-61834] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-ELISA-NBP2-61834-img0001.jpg)
![ELISA: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis of SK-N-SH cells using GRM2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-ELISA-NBP2-61834-img0002.jpg)
![Western Blot: MAP2 Antibody (5B7) [NBP2-37734] - Western blot analysis using MAP2 mAb against human MAP2 recombinant protein. (Observed MW is 36.1 kDa)](http://images.novusbio.com/fullsize/MAP2-Antibody-5B7-Western-Blot-NBP2-37734-img0002.jpg "Western Blot: MAP2 Antibody (5B7) [NBP2-37734] - Western blot analysis using MAP2 mAb against human MAP2 recombinant protein. (Observed MW is 36.1 kDa)")
![Flow Cytometry: MAP2 Antibody (5B7) [NBP2-37734] - Flow cytometric analysis of HeLa cells using MAP2 Antibody (5B7) (green) and negative control (red).](http://images.novusbio.com/fullsize/MAP2-Antibody-5B7-Flow-Cytometry-NBP2-37734-img0003.jpg)
![ELISA: MAP2 Antibody (5B7) [NBP2-37734] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MAP2-Antibody-5B7-ELISA-NBP2-37734-img0001.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)
![Western Blot: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Western blot analysis of Galactosylceramidase/GALC on different lysates with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Rat brain tissue lysate Lane 10: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 60 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32361) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32361_rabbit-galactosylceramidase-galc-mab-psh03-45-278202416285223.jpg "Western Blot: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Western blot analysis of Galactosylceramidase/GALC on different lysates with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Rat brain tissue lysate Lane 10: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 60 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32361) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Immunocytochemistry analysis of A549 cells labeling Galactosylceramidase/GALC with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32361_rabbit-galactosylceramidase-galc-mab-psh03-45-29202410234291.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)
![Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Flow cytometric analysis of Human Park7/DJ-1 expression on HeLa cells. The cells were treated, stained with NBP3-05805, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-235202521451748.png "Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Flow cytometric analysis of Human Park7/DJ-1 expression on HeLa cells. The cells were treated, stained with NBP3-05805, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody (007) [NBP3-05805] - Immunofluorescence staining of Human Park7/DJ-1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-05805 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-23520251628125.png)
![Western Blot: Park7/DJ-1 Antibody (007) [NBP3-05805] - NBP3-05805 at 1:1000 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:20 kDa Observed band size:24 kDa](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-23520252135168.png)
![Immunoprecipitation: Park7/DJ-1 Antibody (007) [NBP3-05805] - PARK7 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg 293T Whole Cell Lysate 2 uL NBP3-05805 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-05805, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 20 kDa Observed band size: 20 kDa](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-235202514331514.png)