| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93396-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38638-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35567-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05413-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99740-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06427-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99490-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99642-50ul | |||
![Western Blot: PTPRD Antibody [NBP2-93396] - Analysis of extracts of various cell lines, using PTPRD at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 3min.](http://images.novusbio.com/fullsize/PTPRD-Antibody-Western-Blot-NBP2-93396-img0001.jpg "Western Blot: PTPRD Antibody [NBP2-93396] - Analysis of extracts of various cell lines, using PTPRD at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 3min.")
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NBP3-38638] - Western blot analysis of lysates from NIH/3T3 cells, using NFkB p105/p50 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-38638_rabbit-nfkb-p105-p50-p-ser-337-pab-101220241154559.jpg "Western Blot: NFkB p105/p50 [p Ser337] Antibody [NBP3-38638] - Western blot analysis of lysates from NIH/3T3 cells, using NFkB p105/p50 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Western Blot: BAALC Antibody [NBP3-35567] - Western blot analysis of various lysates using BAALC Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35567_rabbit-baalc-pab-912202414213748.jpg "Western Blot: BAALC Antibody [NBP3-35567] - Western blot analysis of various lysates using BAALC Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: Olig1 Antibody (7E4B2) [NBP3-15326] - Western blot analysis of extracts of various cell lines, using Olig1 Rabbit mAb (NBP3-15326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Olig1-Antibody-7E4B2-Western-Blot-NBP3-15326-img0001.jpg "Western Blot: Olig1 Antibody (7E4B2) [NBP3-15326] - Western blot analysis of extracts of various cell lines, using Olig1 Rabbit mAb (NBP3-15326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Bioactivity: Recombinant Human beta-Catenin His (C-Term) Protein [NBP3-18198] - Immobilized Human CD31 at 1 ug/mL (100 uL/well) can bind Human beta-Catenin with a linear range of 0.3-4.9ug/ml.](http://images.novusbio.com/fullsize/Recombinant-Human-beta-Catenin-His-C-Term-Protein-Bioactivity-NBP3-18198-img0002.jpg "Bioactivity: Recombinant Human beta-Catenin His (C-Term) Protein [NBP3-18198] - Immobilized Human CD31 at 1 ug/mL (100 uL/well) can bind Human beta-Catenin with a linear range of 0.3-4.9ug/ml.")
![SDS-Page: Recombinant Human beta-Catenin His (C-Term) Protein [NBP3-18198] - Recombinant Human beta-Catenin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-110 kDa.](http://images.novusbio.com/fullsize/Recombinant-Human-beta-Catenin-His-C-Term-Protein-SDS-Page-NBP3-18198-img0001.jpg)
![Western Blot: beta-Catenin [p Thr41, p Ser45] Antibody [NBP3-05413] - Western blot analysis of extracts from SW626 cells using beta-Catenin antibody (NBP3-05413). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Thr41--p-Ser45]-Antibody-Western-Blot-NBP3-05413-img0001.jpg "Western Blot: beta-Catenin [p Thr41, p Ser45] Antibody [NBP3-05413] - Western blot analysis of extracts from SW626 cells using beta-Catenin antibody (NBP3-05413). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: CRABP2 Antibody [NBP2-99740] - Anti-CRABP2 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 14 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-99740-img0004.jpg "Western Blot: CRABP2 Antibody [NBP2-99740] - Anti-CRABP2 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 14 kDa")
![Western Blot: CRABP2 Antibody [NBP2-99740] - CRABP2 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate 2 ul anti-CRABP2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CRABP2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 16 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-99740-img0003.jpg)
![Flow Cytometry: USP7 Antibody (03) [NBP3-06427] - Flow cytometric analysis of Human USP7 expression on Jurkat cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human USP7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/USP7-Antibody-03-Flow-Cytometry-NBP3-06427-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: USP7 Antibody (03) [NBP3-06427] - Flow cytometric analysis of Human USP7 expression on Jurkat cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human USP7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Western Blot: 14-3-3 eta Antibody [NBP2-99490] - Anti-14-3-3 eta rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: A375 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDax](http://images.novusbio.com/fullsize/14-3-3-eta-Antibody-Western-Blot-NBP2-99490-img0003.jpg "Western Blot: 14-3-3 eta Antibody [NBP2-99490] - Anti-14-3-3 eta rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: A375 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDax")
![Immunoprecipitation: 14-3-3 eta Antibody [NBP2-99490] - Lane A:0.5 mg A375 Whole Cell Lysate4 uL anti-YWHAH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-YWHAH rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size :28 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99490_rabbit-polyclonal-14-3-3-eta-antibody-immunoprecipitation-287202374410.jpg)
![Western Blot: 14-3-3 eta Antibody [NBP2-99642] - Anti-14-3-3 eta rabbit polyclonal antibody at 1:500 dilution. Lane A: U937 Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 31 kDa](http://images.novusbio.com/fullsize/14-3-3-eta-Antibody-Western-Blot-NBP2-99642-img0003.jpg "Western Blot: 14-3-3 eta Antibody [NBP2-99642] - Anti-14-3-3 eta rabbit polyclonal antibody at 1:500 dilution. Lane A: U937 Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 31 kDa")
![Immunoprecipitation: 14-3-3 eta Antibody [NBP2-99642] - 14-3-3 eta was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg k562 Whole Cell Lysate. Lane D:0.5 mg NIH-3T3 Whole Cell Lysate. 2 ul anti-14-3-3 eta rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 eta rabbit polyclonal antibody, at 1:2000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDa](http://images.novusbio.com/fullsize/14-3-3-eta-Antibody-Immunoprecipitation-NBP2-99642-img0004.jpg)
![Western Blot: Hyaluronan synthase 1 Antibody [NBP2-85085] - WB Suggested Anti-HAS1 Antibody. Titration: 1.0 ug/ml. Positive Control: THP-1 Whole Cell](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-Western-Blot-NBP2-85085-img0001.jpg "Western Blot: Hyaluronan synthase 1 Antibody [NBP2-85085] - WB Suggested Anti-HAS1 Antibody. Titration: 1.0 ug/ml. Positive Control: THP-1 Whole Cell")
![Western Blot: Hyaluronan synthase 1 Antibody [NBP2-85085] - Host: Rabbit. Target Name: HAS1. Sample Tissue: Human HT1080 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-Western-Blot-NBP2-85085-img0002.jpg)
![Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 55 kDa Observed band size: 110/140 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32777_rabbit-mdm2-hdm2-p-ser166-mab-je64-20-288202412461655.jpg "Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 55 kDa Observed band size: 110/140 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")