| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54450-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26612-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26492-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26698-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26471-50ul | |||
![Western Blot: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TRPC3-Antibody-Western-Blot-NBP2-32258-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for IP at 6 ug per reaction. TRPC3 was also immunoprecipitated by rabbit anti-TRPC3 antibody BL16089. For blotting immunoprecipitated TRPC3, NBP2-32258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TRPC3-Antibody-Immunoprecipitation-NBP2-32258-img0002.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody (CRASH/1289) [NBP2-47648] - Formalin-fixed, paraffin-embedded human Uterus stained with ASRGL1 Monoclonal Antibody (CRASH/1289).](http://images.novusbio.com/fullsize/ASRGL1-Antibody-CRASH-1289-Immunohistochemistry-Paraffin-NBP2-47648-img0003.jpg "Immunohistochemistry-Paraffin: ASRGL1 Antibody (CRASH/1289) [NBP2-47648] - Formalin-fixed, paraffin-embedded human Uterus stained with ASRGL1 Monoclonal Antibody (CRASH/1289).")
![Immunohistochemistry-Paraffin: ASRGL1 Antibody (CRASH/1289) [NBP2-47648] - Formalin-fixed, paraffin-embedded human Fallopian Tube stained with ASRGL1 Monoclonal Antibody (CRASH/1289).](http://images.novusbio.com/fullsize/ASRGL1-Antibody-CRASH-1289-Immunohistochemistry-Paraffin-NBP2-47648-img0001.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody (CRASH/1289) [NBP2-47648] - Formalin-fixed, paraffin-embedded human Endometrium stained with ASRGL1 Monoclonal Antibody (CRASH/1289).](http://images.novusbio.com/fullsize/ASRGL1-Antibody-CRASH-1289-Immunohistochemistry-Paraffin-NBP2-47648-img0002.jpg)
![Immunohistochemistry: CRMP2 Antibody (20D8) [NBP3-26612] - Image of CRMP2 Antibody (20D8) diluted at 1:50 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.35% DAB.](http://images.novusbio.com/fullsize/nbp3-26612_rabbit-crmp2-mab-20d8-262202414471542.jpg "Immunohistochemistry: CRMP2 Antibody (20D8) [NBP3-26612] - Image of CRMP2 Antibody (20D8) diluted at 1:50 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.35% DAB.")
![Immunocytochemistry/Immunofluorescence: CRMP2 Antibody (20D8) [NBP3-26612] - Staining of SH-SY5Y with CRMP2 Antibody (20D8) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 506-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26612_rabbit-crmp2-mab-20d8-282202412493633.jpg)
![Flow Cytometry: CRMP2 Antibody (20D8) [NBP3-26612] - Overlay Peak curve showing SH-SY5Y cells stained with CRMP2 Antibody (20D8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26612_rabbit-crmp2-mab-20d8-282202414183684.jpg)
![Immunohistochemistry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Image of SERCA2 ATPase Antibody (20D1) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-2722024820398.jpg "Immunohistochemistry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Image of SERCA2 ATPase Antibody (20D1) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.")
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Staining of MCF7 with SERCA2 ATPase Antibody (20D1) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 509-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-28220241247516.jpg)
![Flow Cytometry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Overlay Peak curve showing Hela cells stained with SERCA2 ATPase Antibody (20D1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-282202414312788.jpg)
![Western Blot: TMEM59L Antibody [NBP2-20664] - Sample (30 ug of whole cell lysate) A: Neuro2A 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/TMEM59L-Antibody-Western-Blot-NBP2-20664-img0001.jpg "Western Blot: TMEM59L Antibody [NBP2-20664] - Sample (30 ug of whole cell lysate) A: Neuro2A 10% SDS PAGE gel, diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: TMEM59L Antibody [NBP2-20664] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/TMEM59L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20664-img0002.jpg)
![Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-262202414471523.jpg "Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.")
![Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-26220241447154.jpg)
![Western Blot: Caspase-3 Antibody (14C1) [NBP3-26698] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate. All lanes: Caspase-3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32 kDa](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-282202411442633.jpg)
![Western Blot: OMgp Antibody [NBP1-33647] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/OMgp-Antibody-Western-Blot-NBP1-33647-img0003.jpg "Western Blot: OMgp Antibody [NBP1-33647] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Frozen: OMgp Antibody [NBP1-33647] - OMG antibody [C1C2] detects OMG protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: OMG antibody [C1C2] diluted at 1:1250. Blue: DAPI](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Frozen-NBP1-33647-img0004.jpg)
![Immunohistochemistry: Complement C3 Antibody (4D12) [NBP3-26471] - Image of Complement C3 Antibody (4D12) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-262202415475630.jpg "Immunohistochemistry: Complement C3 Antibody (4D12) [NBP3-26471] - Image of Complement C3 Antibody (4D12) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Complement C3 Antibody (4D12) [NBP3-26471] - Positive Western Blot detected in: THP-1 whole cell lysate, K562 whole cell lysate. All lanes: Complement C3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 188 kDa Observed band size: 115 kDa](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-28220241140917.jpg)
![Flow Cytometry: Complement C3 Antibody (4D12) [NBP3-26471] - Overlay histogram showing HepG2 cells stained with Complement C3 Antibody (4D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-282202414183687.jpg)