| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50537-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98743-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81074-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27158-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34563-0.1mg | |||
![Western Blot: beta-Catenin [p Ser45] Antibody [NBP3-05442] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-05442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser45]-Antibody-Western-Blot-NBP3-05442-img0001.jpg "Western Blot: beta-Catenin [p Ser45] Antibody [NBP3-05442] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-05442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of C2C12 cells, using beta-Catenin pAb (NBP3-05441) at 1:1000 dilution or beta-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser552]-Antibody-Western-Blot-NBP3-05441-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of C2C12 cells, using beta-Catenin pAb (NBP3-05441) at 1:1000 dilution or beta-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.")
![<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of various cell lines, using beta-Catenin [p Ser552] antibody (NBP3-05441) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser552]-Antibody-Western-Blot-NBP3-05441-img0001.jpg)
![Immunohistochemistry-Paraffin: Calcitonin Antibody (SP17) [NBP2-26483] - Formalin fixed paraffin embedded human thyroid medullary carcinoma stained with Calcitonin antibody (NBP2-26483).](http://images.novusbio.com/fullsize/Calcitonin-Antibody-SP17-Immunohistochemistry-Paraffin-NBP2-26483-img0008.jpg "Immunohistochemistry-Paraffin: Calcitonin Antibody (SP17) [NBP2-26483] - Formalin fixed paraffin embedded human thyroid medullary carcinoma stained with Calcitonin antibody (NBP2-26483).")
![Immunohistochemistry-Paraffin: Calcitonin Antibody (SP17) [NBP2-26483] - Human Pituitary.](http://images.novusbio.com/fullsize/Calcitonin-Antibody-SP17-Immunohistochemistry-Paraffin-NBP2-26483-img0005.jpg)
![Immunohistochemistry-Paraffin: Calcitonin Antibody (SP17) [NBP2-26483] - Human Lung Adenocarcinoma.](http://images.novusbio.com/fullsize/Calcitonin-Antibody-SP17-Immunohistochemistry-Paraffin-NBP2-26483-img0007.jpg)
![Western Blot: CRABP2 Antibody (JE65-66) [NBP3-32214] - Western blot analysis of CRABP2 on MCF-7 cell lysates with Rabbit anti-CRABP2 antibody (NBP3-32214) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 16 kDa Observed band size: 14 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32214) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32214_rabbit-crabp2-mab-je65-66-278202415242666.jpg "Western Blot: CRABP2 Antibody (JE65-66) [NBP3-32214] - Western blot analysis of CRABP2 on MCF-7 cell lysates with Rabbit anti-CRABP2 antibody (NBP3-32214) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 16 kDa Observed band size: 14 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32214) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CRABP2 Antibody (JE65-66) [NBP3-32214] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CRABP2 antibody (NBP3-32214) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32214) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32214_rabbit-crabp2-mab-je65-66-298202414161824.jpg)
![Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-288202412442975.jpg "Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-29202410234274.jpg)
![Western Blot: Nucleostemin Antibody [NBP2-98743] - Anti-Nucleostemin rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NBP2-98743-img0002.jpg "Western Blot: Nucleostemin Antibody [NBP2-98743] - Anti-Nucleostemin rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: Nucleostemin Antibody [NBP2-98743] - Immunofluorescence staining of Nucleostemin in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98743 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green)Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98743_rabbit-polyclonal-nucleostemin-antibody-235202514331528.png)
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (MNAC13) [NBP2-81074] - Immunofluoresence staining of fixed HeLa cells with anti-TrkA antibody (MNAC13) [NBP2-81074]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of MNAC13 [NBP2-81074] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81074, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TrkA-Antibody-MNAC13-Immunocytochemistry-Immunofluorescence-NBP2-81074-img0001.jpg "Immunocytochemistry/Immunofluorescence: TrkA Antibody (MNAC13) [NBP2-81074] - Immunofluoresence staining of fixed HeLa cells with anti-TrkA antibody (MNAC13) [NBP2-81074]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of MNAC13 [NBP2-81074] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81074, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: Hyaluronan synthase 1 Antibody (2G5H6) [NBP3-27158] - Analysis using Hyaluronan synthase 1 mAb against HEK293 (1) and Hyaluronan synthase 1 (AA: 74-399)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27158_mouse-hyaluronan-synthase-1-mab-2g5h6-bsa-free-1042024917036.jpg%3Fv=1666704596 "Western Blot: Hyaluronan synthase 1 Antibody (2G5H6) [NBP3-27158] - Analysis using Hyaluronan synthase 1 mAb against HEK293 (1) and Hyaluronan synthase 1 (AA: 74-399)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: Hyaluronan synthase 1 Antibody (2G5H6) [NBP3-27158] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27158_mouse-hyaluronan-synthase-1-mab-2g5h6-bsa-free-1042024920217.jpg%3Fv=1666704596)
![Western Blot: Hyaluronan synthase 1 Antibody (2G5H6) [NBP3-27158] - Analysis using Hyaluronan synthase 1 mAb against human Hyaluronan synthase 1 (AA: 74-399) recombinant protein. (Expected MW is 40.2 kDa)](http://images.novusbio.com/fullsize/nbp3-27158_mouse-hyaluronan-synthase-1-mab-2g5h6-bsa-free-1042024921157.jpg%3Fv=1666704596)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - IHC-Prediluted [NBP2-44980] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-+-DO-7-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-44980-img0001.jpg "Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - IHC-Prediluted [NBP2-44980] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)")
![Western Blot: MDM2/HDM2 [p Ser185] Antibody [NB100-1683] - Shown in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/MDM2-HDM2 [p Ser185] Antibody-Western Blot-NB100-1683-img0003.jpg "Western Blot: MDM2/HDM2 [p Ser185] Antibody [NB100-1683] - Shown in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.")
