| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90271-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26698-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26697-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21860-50ul | |||
![Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-278202415242680.jpg "Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32458) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-298202414333652.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0002.jpg "Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunofluorescence staining of AIF-1/Iba1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90271 (dilution ratio 1:60) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90271_rabbit-monoclonal-aif-1-iba1-antibody-031-235202514492357.png)
![Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-278202416285257.jpg "Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ATG12 Antibody (JE30-61) [NBP3-32056] - Immunocytochemistry analysis of HCT 116 cells labeling ATG12 with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-2920241192327.jpg)
![Flow Cytometry: ATG12 Antibody (JE30-61) [NBP3-32056] - Flow cytometric analysis of HCT 116 cells labeling ATG12. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32056, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-29202416453286.jpg)
![Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32283_mouse-dynamin-mab-a1b2-r-27820241631653.jpg "Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dynamin Antibody (A1B2-R) [NBP3-32283] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32283) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32283_mouse-dynamin-mab-a1b2-r-298202414304193.jpg)
![Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-262202414471523.jpg "Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.")
![Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-26220241447154.jpg)
![Western Blot: Caspase-3 Antibody (14C1) [NBP3-26698] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate. All lanes: Caspase-3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32 kDa](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-282202411442633.jpg)
![Western Blot: Caspase-9 Antibody (2D5) [NBP3-26697] - Positive Western Blot detected in: HL-60 whole cell lysate, LO2 whole cell lysate. All lanes: Caspase-9 Antibody at 1.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 31, 18, 37 KDa Observed band size: 47 KDa](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-28220241140940.jpg "Western Blot: Caspase-9 Antibody (2D5) [NBP3-26697] - Positive Western Blot detected in: HL-60 whole cell lysate, LO2 whole cell lysate. All lanes: Caspase-9 Antibody at 1.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 31, 18, 37 KDa Observed band size: 47 KDa")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (2D5) [NBP3-26697] - Staining of HepG2 cells with Caspase-9 Antibody (2D5) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-28220241323614.jpg)
![Flow Cytometry: Caspase-9 Antibody (2D5) [NBP3-26697] - Overlay histogram showing K562 cells stained with Caspase-9 Antibody (2D5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-282202414183649.jpg)
![Western Blot: URM1 Antibody - BSA Free [NBP3-45632] - Human placenta tissue were subjected to SDS PAGE followed by western blot with (URM1 antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-45632_rabbit-urm1-pab-bsa-free-28420251385038.jpg "Western Blot: URM1 Antibody - BSA Free [NBP3-45632] - Human placenta tissue were subjected to SDS PAGE followed by western blot with (URM1 antibody) at dilution of 1:1000")
![Immunohistochemistry: URM1 Antibody - BSA Free [NBP3-45632] - Immunohistochemistry of paraffin-embedded human liver cancer using (URM1 antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-45632_rabbit-urm1-pab-bsa-free-284202512365447.jpg)
![Western Blot: XK X-linked Kx blood group Antibody - BSA Free [NBP3-45540] - Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with (XK X-linked Kx blood group Antibody) at dilution of 1:600](http://images.novusbio.com/fullsize/nbp3-45540_rabbit-xk-x-linked-kx-blood-group-pab-bsa-free-284202512582675.jpg "Western Blot: XK X-linked Kx blood group Antibody - BSA Free [NBP3-45540] - Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with (XK X-linked Kx blood group Antibody) at dilution of 1:600")
![Immunohistochemistry: XK X-linked Kx blood group Antibody - BSA Free [NBP3-45540] - Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using (XK X-linked Kx blood group Antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-45540_rabbit-xk-x-linked-kx-blood-group-pab-bsa-free-284202512582681.jpg)
![Western Blot: AIF-1/Iba1 Antibody (SR1261) [NBP3-21860] - Western blot analysis using NBP3-21860 in U937 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21860_rabbit-aif-1-iba1-mab-sr1261-5720231512345.jpg "Western Blot: AIF-1/Iba1 Antibody (SR1261) [NBP3-21860] - Western blot analysis using NBP3-21860 in U937 cell lysate.")