| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81418-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56234-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56205-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21749-50ul | |||
![Immunocytochemistry/Immunofluorescence: ZDHHC8 Antibody [NBP1-81418] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/ZDHHC8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81418-img0004.jpg "Immunocytochemistry/Immunofluorescence: ZDHHC8 Antibody [NBP1-81418] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: ZDHHC8 Antibody [NBP1-81418] - Staining of human kidney shows strong cytoplasmic and extracellular positivity in cells in tubules.](http://images.novusbio.com/fullsize/ZDHHC8-Antibody-Immunohistochemistry-Paraffin-NBP1-81418-img0005.jpg)
![Western Blot: p53 Antibody [NBP2-56234] - Analysis in human cell line U-251 MG and human cell line PC-3.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-56234-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-56234] - Analysis in human cell line U-251 MG and human cell line PC-3.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-56234] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56234-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PDE6 beta Antibody [NBP2-56205] - Staining of human cell line SH-SY5Y shows localization to cytosol.](http://images.novusbio.com/fullsize/PDE6-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56205-img0001.jpg "Immunocytochemistry/Immunofluorescence: PDE6 beta Antibody [NBP2-56205] - Staining of human cell line SH-SY5Y shows localization to cytosol.")
![Immunohistochemistry-Paraffin: PDE6 beta Antibody [NBP2-56205] - Staining of human retina shows strong cytoplasmic positivity in photoreceptor cells and outer plexiform layer.](http://images.novusbio.com/fullsize/PDE6-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-56205-img0004.jpg)
![Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-5220251733576.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: p53 Antibody (9D6F7) [p53] - Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-52202517344284.jpg)
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Western Blot: PRAS40 Antibody (SR1679) [NBP3-21749] - Western blot analysis using NBP3-21749 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21749_rabbit-pras40-mab-sr1679-5720231624749.jpg "Western Blot: PRAS40 Antibody (SR1679) [NBP3-21749] - Western blot analysis using NBP3-21749 in 293T cell lysate.")
![Western Blot: p53 [p Ser33] Antibody [NBP3-13309] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser33) antibody (NBP3-13309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser33]-Antibody-Western-Blot-NBP3-13309-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser33] Antibody [NBP3-13309] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser33) antibody (NBP3-13309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Cadherin-8 Antibody [NBP1-59221] - Human Fetal Heart Lysate, concentration 1.0ug/ml.](http://images.novusbio.com/fullsize/Cadherin-8-Antibody-Western-Blot-NBP1-59221-img0001.jpg "Western Blot: Cadherin-8 Antibody [NBP1-59221] - Human Fetal Heart Lysate, concentration 1.0ug/ml.")
![Immunohistochemistry-Paraffin: Cadherin-8 Antibody [NBP1-59221] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Cadherin-8-Antibody-Immunohistochemistry-Paraffin-NBP1-59221-img0003.jpg)
![Western Blot: PRAS40 Antibody [NBP1-55261] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate](http://images.novusbio.com/fullsize/PRAS40-Antibody-Western-Blot-NBP1-55261-img0004.jpg "Western Blot: PRAS40 Antibody [NBP1-55261] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate")
![Immunohistochemistry: PRAS40 Antibody [NBP1-55261] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic in endothelial cells in blood vessels Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-NBP1-55261-img0003.jpg)