| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54317-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26697-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47668-0.1mg | |||
| IHC-Prediluted | NBP2-48291 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54569-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26305-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26492-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26236-50ul | |||
![Immunohistochemistry-Paraffin: TSHR Antibody (SPM222) [NBP2-53224] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with TSHRB Monoclonal Antibody (SPM222).](http://images.novusbio.com/fullsize/TSHR-Antibody-SPM222-Immunohistochemistry-Paraffin-NBP2-53224-img0001.jpg "Immunohistochemistry-Paraffin: TSHR Antibody (SPM222) [NBP2-53224] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with TSHRB Monoclonal Antibody (SPM222).")
![Western Blot: Caspase-9 Antibody (2D5) [NBP3-26697] - Positive Western Blot detected in: HL-60 whole cell lysate, LO2 whole cell lysate. All lanes: Caspase-9 Antibody at 1.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 31, 18, 37 KDa Observed band size: 47 KDa](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-28220241140940.jpg "Western Blot: Caspase-9 Antibody (2D5) [NBP3-26697] - Positive Western Blot detected in: HL-60 whole cell lysate, LO2 whole cell lysate. All lanes: Caspase-9 Antibody at 1.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 31, 18, 37 KDa Observed band size: 47 KDa")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (2D5) [NBP3-26697] - Staining of HepG2 cells with Caspase-9 Antibody (2D5) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-28220241323614.jpg)
![Flow Cytometry: Caspase-9 Antibody (2D5) [NBP3-26697] - Overlay histogram showing K562 cells stained with Caspase-9 Antibody (2D5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-282202414183649.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (SPM145) [NBP2-45297] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM145).](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM145-Immunohistochemistry-Paraffin-NBP2-45297-img0001.jpg "Immunohistochemistry-Paraffin: NF-H Antibody (SPM145) [NBP2-45297] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM145).")
![Immunohistochemistry-Paraffin: TSHR Antibody (SPM223) [NBP2-53217] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with TSHRA Monoclonal Antibody (SPM223).](http://images.novusbio.com/fullsize/TSHR-Antibody-SPM223-Immunohistochemistry-Paraffin-NBP2-53217-img0001.jpg "Immunohistochemistry-Paraffin: TSHR Antibody (SPM223) [NBP2-53217] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with TSHRA Monoclonal Antibody (SPM223).")
![Immunohistochemistry: SOX2 Antibody (2E1) [NBP3-26305] - Image of SOX2 Antibody (2E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26305_rabbit-sox2-mab-2e1-27220248274918.jpg "Immunohistochemistry: SOX2 Antibody (2E1) [NBP3-26305] - Image of SOX2 Antibody (2E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: SOX2 Antibody (2E1) [NBP3-26305] - Positive Western Blot detected in: U-251 whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: SOX2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/nbp3-26305_rabbit-sox2-mab-2e1-28220241148581.jpg)
![Immunohistochemistry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Image of SERCA2 ATPase Antibody (20D1) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-2722024820398.jpg "Immunohistochemistry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Image of SERCA2 ATPase Antibody (20D1) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.")
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Staining of MCF7 with SERCA2 ATPase Antibody (20D1) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 509-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-28220241247516.jpg)
![Flow Cytometry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Overlay Peak curve showing Hela cells stained with SERCA2 ATPase Antibody (20D1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-282202414312788.jpg)
![Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-26220241322159.jpg "Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).")
![Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] - Image of HDAC6 Antibody (2H12) diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-262202414471573.jpg)
![Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate. All lanes: HDAC6 Antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 132, 115 KDa Observed band size: 160 KDa](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-282202411413619.jpg)
![Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/IRS2-Antibody-Immunohistochemistry-Paraffin-NB100-68244-img0002.jpg "Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: IRS2 Antibody [NB100-68244] - Detection of Human IRS2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IRS2 antibody NB100-68244 used for IP at 3 mcg/mg lysate. IRS2 was also immunoprecipitated by rabbit anti-IRS2 antibodies NB100-68242 and NB100-68243, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/IRS2-Antibody-Immunoprecipitation-NB100-68244-img0003.jpg)
![Western Blot: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TRPC3-Antibody-Western-Blot-NBP2-32258-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for IP at 6 ug per reaction. TRPC3 was also immunoprecipitated by rabbit anti-TRPC3 antibody BL16089. For blotting immunoprecipitated TRPC3, NBP2-32258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TRPC3-Antibody-Immunoprecipitation-NBP2-32258-img0002.jpg)