| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72948 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47610-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto594 | NBP3-14765-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89201-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47945-0.1mg | |||
| IHC-Prediluted | NBP2-48086 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47950-0.1mg | |||
| IHC-Prediluted | NBP2-48191 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84826-25ul | |||
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16589] - HBL435 xenograft, using Galectin3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16589-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16589] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0004.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Galectin-3 antibody detects Galectin-3 protein by western blot analysis. A. 50 ug rat colon lysate/extract. 12% SDS-PAGE. Galectin-3 antibody dilution: 1:10000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0006.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16589] - Sandwich ELISA detection of recombinant full-length Galectin-3 protein using as capture antibody at concentration of 5 ug/mL and Galectin-3 Antibody as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16589-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16589] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16589_rabbit-polyclonal-galectin-3-antibody-1610202419414598.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Wild-type (WT) and Brg1 knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Wild-type (WT) and Brg1 knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brg1 protein stained by Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunocytochemistry-Immunofluorescence-NBP3-13537-img0004.jpg)
![Immunohistochemistry-Frozen: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: Brg1 stained by Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Red: SOX2, stained by SOX2 antibody [N1C3] (NBP1-33766) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunohistochemistry-Frozen-NBP3-13537-img0007.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0001.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0005.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0006.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunohistochemistry-Paraffin-NBP3-13537-img0002.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunohistochemistry-Paraffin-NBP3-13537-img0003.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-910202420122065.jpg "Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis. Sample: Paraffin-embedded Human normal prostate tissue. TET1 antibody [N3C1] (NBP2-76345) dilution: 1:1000.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242063370.jpg)
![Western Blot: TET1 Antibody [NBP2-76345] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (NBP2-76345) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242011503.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020241957212.jpg)
![Immunohistochemistry: TET1 Antibody [NBP2-76345] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (NBP2-76345) antibody at 1:1000 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242014666.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242021956.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . TET1 antibody [N3C1] (NBP2-76345) diluted at 1:250.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242091468.jpg)
![Immunocytochemistry/ Immunofluorescence: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242041232.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TET1 Antibody [NBP2-76345] - Non-transfected (–) and transfected (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-1610202419414597.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (NBP2-76345) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-western-blot-2611202518384013.jpg)
![HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (NBP2-76345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-western-blot-261120251849398.jpg)
![Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Neurogenin 1 monoclonal antibody.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Western-Blot-NBP1-47871-img0020.jpg "Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Neurogenin 1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - COS7 cells transiently transfected by pCMV6-ENTRY Neurogenin-1.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunocytochemistry-Immunofluorescence-NBP1-47871-img0011.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human prostate tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0017.jpg)
![Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Neurogenin 1.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Western-Blot-NBP1-47871-img0019.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human colon tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0012.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human Kidney tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0013.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human lung tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0014.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human Ovary tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0015.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human pancreas tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0016.jpg)
![Immunoprecipitation: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Immunoprecipitation (IP) of NEUROG1 by using TrueMab monoclonal anti-NEUROG1 antibodies (Negative control: IP without adding anti-NEUROG1 antibody.). For each experiment, 500ul of DDK tagged NEUROG1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunoprecipitation-NBP1-47871-img0021.jpg)
![Western Blot: SHANK3 Antibody (S69) [NBP1-47610] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Western-Blot-NBP1-47610-img0005.jpg "Western Blot: SHANK3 Antibody (S69) [NBP1-47610] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Immunocytochemistry-Immunofluorescence-NBP1-47610-img0007.jpg)
![Immunohistochemistry: SHANK3 Antibody (S69) [NBP1-47610] - Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Immunohistochemistry-NBP1-47610-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Immunocytochemistry-Immunofluorescence-NBP1-47610-img0003.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Western-Blot-NBP2-54789-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54789-img0004.jpg)
![Immunohistochemistry: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (NBP2-54789). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 human alpha synuclein PFFs (NBP2-54789) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-54789-img0007.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to active alpha Synuclein aggregate (NBP2-54789) and active alpha Synuclein monomer (NBP2-54788) alone. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-54789-img0006.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0008.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1) (Catalog No. NBP2-54789). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Product-Image-NBP2-54789-img0010.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0009.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0010.jpg "Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0008.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse liver tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0006.jpg)
![Flow Cytometry: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of SH-SY-5Y cells with TrkA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Flow-Cytometry-NBP2-67473-img0001.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on different lysates using anti-TrkA antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: SH-SY-5Y](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0007.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Western blot analysis of TrkA on N2A cell lysates using anti-TrkA antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0003.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded human tonsil tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0004.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse kidney tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0005.jpg)
![Western Blot: Olig2 Antibody (064) [NBP2-89201] - A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa](http://images.novusbio.com/fullsize/Olig2-Antibody-064-Western-Blot-NBP2-89201-img0003.jpg "Western Blot: Olig2 Antibody (064) [NBP2-89201] - A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa")
![Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human brain with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Olig2-Antibody-064-Immunohistochemistry-Paraffin-NBP2-89201-img0002.jpg)
![Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human glioma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Olig2-Antibody-064-Immunohistochemistry-Paraffin-NBP2-89201-img0001.jpg)
![Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Mouse brain cells fixed and stained for Olig2 protein: 1:100. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-89201_rabbit-monoclonal-olig2-antibody-064-flow-cytometry-1052024144135..png)
![Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Rat brain cells fixed and stained with Olig2. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-89201_rabbit-monoclonal-olig2-antibody-064-flow-cytometry-1052024144528..png)
![Western Blot: Dfna5 Antibody [NBP2-80426] - Analysis of Dfna5 on SiHa cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80426, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Dfna5-Antibody-Western-Blot-NBP2-80426-img0006.jpg "Western Blot: Dfna5 Antibody [NBP2-80426] - Analysis of Dfna5 on SiHa cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80426, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in mouse small intestine tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/Dfna5-Antibody-Immunohistochemistry-Paraffin-NBP2-80426-img0005.jpg)
![Flow Cytometry: Dfna5 Antibody [NBP2-80426] - Analysis of Methionine Aminopeptidase 2/METAP2 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Dfna5-Antibody-Flow-Cytometry-NBP2-80426-img0001.jpg)
![Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in rat testis tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed](http://images.novusbio.com/fullsize/Dfna5-Antibody-Immunohistochemistry-Paraffin-NBP2-80426-img0002.jpg)
![Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in human thyroid gland tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/Dfna5-Antibody-Immunohistochemistry-Paraffin-NBP2-80426-img0003.jpg)
![Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in human breast tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, wash](http://images.novusbio.com/fullsize/Dfna5-Antibody-Immunohistochemistry-Paraffin-NBP2-80426-img0004.jpg)
![Western Blot: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Antibody (KRT19/799).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Western-Blot-NBP2-44826-img0009.jpg "Western Blot: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Antibody (KRT19/799).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - ICC/IF analysis of MCF-7 cells labeling Cytokeratin 19 with Conjugated with CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunofluorescence-NBP2-44826-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0005.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Flow Cytometric Analysis of MeOH-fixed MCF-7 cells using Cytokeratin 19 Antibody (KRT19/799) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Flow-Cytometry-NBP2-44826-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Bladder Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Ovarian Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0004.jpg)
![Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Western-Blot-NBP2-44833-img0008.jpg "Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Angiosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Ewing's Sarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Leiomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Melanoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Rhabdomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Uterus stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0006.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84826 (A) shows similar protein distribution across tissues to independent antibody NBP1-84827 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0016.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in control (vector only transfected HEK293T lysate) and ESR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Western-Blot-NBP1-84826-img0007.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0014.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0017.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0018.jpg)
