| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26486-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21612-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20107-0.025mg | |||
![Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: RASSF1A transfected 293T cell lysate](http://images.novusbio.com/fullsize/RASSF1-Antibody-3F3-Western-Blot-NBP1-04342-img0002.jpg "Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: RASSF1A transfected 293T cell lysate")
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (3F3) [NBP1-04342] - Analysis of RASSF1A in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/RASSF1-Antibody-3F3-Immunocytochemistry-Immunofluorescence-NBP1-04342-img0004.jpg)
![Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - Cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysate](http://images.novusbio.com/fullsize/RASSF1-Antibody-3F3-Western-Blot-NBP1-04342-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (3F3) [NBP1-04342] - Analysis of RASSF1A in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/RASSF1-Antibody-3F3-Immunocytochemistry-Immunofluorescence-NBP1-04342-img0003.jpg)
![Western Blot: FKBP38 Antibody (2J7P6) [NBP3-16684] - Western blot analysis of extracts of various cell lines, using FKBP38 Rabbit mAb (NBP3-16684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/FKBP38-Antibody-2J7P6-Western-Blot-NBP3-16684-img0003.jpg "Western Blot: FKBP38 Antibody (2J7P6) [NBP3-16684] - Western blot analysis of extracts of various cell lines, using FKBP38 Rabbit mAb (NBP3-16684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: FKBP38 Antibody (2J7P6) [NBP3-16684] - Western blot analysis of extracts of various cell lines, using FKBP38 Rabbit mAb (NBP3-16684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/FKBP38-Antibody-2J7P6-Western-Blot-NBP3-16684-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: FKBP38 Antibody (2J7P6) [NBP3-16684] - Confocal imaging of NIH/3T3 cells using FKBP38 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16684_rabbit-fkbp38-mab-2j7p6-8220252010492.jpg)
![Western Blot: LATS1 Antibody [NBP3-05224] - Western blot analysis of extracts of various cell lines, using LATS1 antibody (NBP3-05224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LATS1 Antibody-Western Blot-NBP3-05224-img0002.jpg "Western Blot: LATS1 Antibody [NBP3-05224] - Western blot analysis of extracts of various cell lines, using LATS1 antibody (NBP3-05224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP3-05224] - Rat spinal cord using LATS1 Rabbit pAb (NBP3-05224) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP3-05224-img0001.jpg)
![Western Blot: LATS1 Antibody - Azide and BSA Free [LATS1] - Western blot analysis of various lysates using LATS1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-05224_rabbit-polyclonal-lats1-antibody-52202516345232.jpg)
![Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-V152-Flow-Cytometry-NBP3-07017-img0001.jpg "Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Western Blot: NUP98 Antibody (2H4) [NBP3-15200] - Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate. All lanes: NUP98 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 198, 188, 98, 97, 196, 187 KDa. Observed band size: 100 KDa.](http://images.novusbio.com/fullsize/NUP98-Antibody-2H4-Western-Blot-NBP3-15200-img0003.jpg "Western Blot: NUP98 Antibody (2H4) [NBP3-15200] - Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate. All lanes: NUP98 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 198, 188, 98, 97, 196, 187 KDa. Observed band size: 100 KDa.")
![Immunocytochemistry/Immunofluorescence: NUP98 Antibody (2H4) [NBP3-15200] - Immunofluorescence staining of Hela Cells with at NUP98 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/NUP98-Antibody-2H4-Immunocytochemistry-Immunofluorescence-NBP3-15200-img0001.jpg)
![Immunohistochemistry: NUP98 Antibody (2H4) [NBP3-15200] - IHC image of NUP98 antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/NUP98-Antibody-2H4-Immunohistochemistry-NBP3-15200-img0002.jpg)
![Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] - Immunoprecipitating Aurora B in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26486 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-26220241322158.jpg "Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] - Immunoprecipitating Aurora B in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26486 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-262202415253321.jpg)
![Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-2722024827492.jpg)
![Western Blot: Aurora B Antibody (4E5) [NBP3-26486] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: Aurora B Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 36, 17, 35 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-282202411442635.jpg)
![Western Blot: NEK2 Antibody [NBP1-78022] - Western blot analysis is shown using Affinity Purified NEK2 antibody to detect enCanineenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/NEK2-Antibody-Western-Blot-NBP1-78022-img0005.jpg "Western Blot: NEK2 Antibody [NBP1-78022] - Western blot analysis is shown using Affinity Purified NEK2 antibody to detect enCanineenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.")
![Western Blot: NEK2 Antibody [NBP1-78022] - Western Blot of NEK2 antibody. Lane 1: Opal Pre-stained Molecular Weight Marker. Lane 2: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (50ng). Lane 3: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (20ng). Lane 4: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (20ng). Lane 5: Jurkat cell lysate (10ug). Primary Antibody: Anti-NEK2 at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30mins at RT. Block: BlockOut Buffer. Expect/Observed MW: ~76kDa rec NEK2.](http://images.novusbio.com/fullsize/NEK2-Antibody-Western-Blot-NBP1-78022-img0004.jpg)
![N/A: NEK2 Antibody [NBP1-78022]](http://images.novusbio.com/fullsize/NEK2-Antibody-N-A-NBP1-78022-img0003.jpg)
![Western Blot: TPX2 Antibody (S01-8A6) [NBP3-19922] - Western blot detection of TPX2 in Hela, A549, HL-60 using NBP3-19922 (1:1000 diluted)](http://images.novusbio.com/fullsize/TPX2-Antibody-S01-8A6-Western-Blot-NBP3-19922-img0003.jpg "Western Blot: TPX2 Antibody (S01-8A6) [NBP3-19922] - Western blot detection of TPX2 in Hela, A549, HL-60 using NBP3-19922 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: TPX2 Antibody (S01-8A6) [NBP3-19922] - Immunohistochemistry of TPX2 in paraffin-embedded Human lung cancer tissue using NBP3-19922 at dilution 1/50](http://images.novusbio.com/fullsize/TPX2-Antibody-S01-8A6-Immunohistochemistry-Paraffin-NBP3-19922-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TPX2 Antibody (S01-8A6) [NBP3-19922] - Immunocytochemistry of TPX2 (green) in Hela using NBP3-19922 at dilution 1/2, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19922_rabbit-tpx2-mab-s01-8a6-113202515145451.jpg)
![Western Blot: PRC1 Antibody (SR2177) [NBP3-21612] - Western blot analysis using NBP3-21612 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21612_rabbit-prc1-mab-sr2177-672023106287.jpg "Western Blot: PRC1 Antibody (SR2177) [NBP3-21612] - Western blot analysis using NBP3-21612 in HeLa cell lysate.")
![Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.](http://images.novusbio.com/fullsize/PLK1-[p-Ser482--p-Ser486--p-Ser490]-Antibody-Western-Blot-NB300-278-img0001.jpg "Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.")
![Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924254.png "Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (NBP3-20107, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023964610.png)
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41A-1 (NBP3-20107) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20107, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924231.png)