| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21644-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38500-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54776-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04486-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26505-50ul | |||
![Western Blot: HRAS Antibody (SR1327) [NBP3-21644] - Western blot analysis MCF7 cell lysate using NBP3-21644.](http://images.novusbio.com/fullsize/nbp3-21644_rabbit-hras-mab-sr1327-672023106249.jpg "Western Blot: HRAS Antibody (SR1327) [NBP3-21644] - Western blot analysis MCF7 cell lysate using NBP3-21644.")
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-1572024145956..jpg "Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE-1 antibody at 1/1,000 dilution.Lane 1: A549-si NT cell lysateLane 2: A549-si LYVE-1 cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-66940 was shown to specifically react with LYVE-1 in A549-si NT cells. Weakened band was observed when A549-si LYVE-1 sample was tested. A549-si NT and Hela-si LYVE-1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-29, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-15720241537..jpg)
![Flow Cytometry: LYVE-1 Antibody (JF0979) [NBP2-66940] - Analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/LYVE-1-Antibody-JF0979-Flow-Cytometry-NBP2-66940-img0001.jpg)
![Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-278202415295577.jpg "Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HRAS Antibody (JE42-15) [NBP3-32413] - Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-298202414161896.jpg)
![Western Blot: LYVE-1 Antibody [NBP2-38500] - Analysis in human placenta tissue.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Western-Blot-NBP2-38500-img0003.jpg "Western Blot: LYVE-1 Antibody [NBP2-38500] - Analysis in human placenta tissue.")
![Immunohistochemistry-Paraffin: LYVE-1 Antibody [NBP2-38500] - Staining of human rectum shows distinct cytoplasmic positivity in lymph vessels.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Immunohistochemistry-NBP2-38500-img0001.jpg)
![Western Blot: Snail [p Ser246] Antibody [NBP2-54776] - analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody. The lane on the right is treated with the antigen-specific peptide.](http://images.novusbio.com/fullsize/Snail-[p-Ser246]-Antibody-Western-Blot-NBP2-54776-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Snail [p Ser246] Antibody [NBP2-54776] - analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody. The lane on the right is treated with the antigen-specific peptide.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Snail [p Ser246] Antibody - BSA Free [NBP2-54776] - Immunofluorescence staining of methanol-fixed HuvEc cells using Snail (Phospho-Ser246) Antibody.](http://images.novusbio.com/fullsize/nbp2-54776_rabbit-polyclonal-snail-p-ser246-antibody-2711202419541572.jpg)
![Western Blot: HRAS Antibody [NBP3-04486] - Analysis of extracts of mouse brain, using HRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP3-04486-img0002.jpg "Western Blot: HRAS Antibody [NBP3-04486] - Analysis of extracts of mouse brain, using HRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit")
![Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP3-04486] - Analysis of HeLa cells using HRAS antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04486-img0001.jpg)
![Immunohistochemistry: APC Antibody (5F2) [NBP3-26505] - Image of APC Antibody (5F2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26505_rabbit-apc-mab-5f2-27220248471917.jpg "Immunohistochemistry: APC Antibody (5F2) [NBP3-26505] - Image of APC Antibody (5F2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: APC Antibody (5F2) [NBP3-26505] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate. All lanes: APC Antibody at 1:1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 312, 301, 309 kDa Observed band size: 160 kDa](http://images.novusbio.com/fullsize/nbp3-26505_rabbit-apc-mab-5f2-282202411485817.jpg)
![Western Blot: TIMP-3 Antibody (PSH04-41) [NBP3-33023] - Western blot analysis of TIMP-3 on different lysates with Rabbit anti-TIMP-3 antibody (NBP3-33023) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: A431 cell lysate Lane 3: Human liver tissue lysate (negative) Lane 4: Rat placenta tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 20 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33023) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33023_rabbit-timp-3-mab-psh04-41-288202412493444.jpg "Western Blot: TIMP-3 Antibody (PSH04-41) [NBP3-33023] - Western blot analysis of TIMP-3 on different lysates with Rabbit anti-TIMP-3 antibody (NBP3-33023) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: A431 cell lysate Lane 3: Human liver tissue lysate (negative) Lane 4: Rat placenta tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 20 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33023) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TIMP-3 Antibody (PSH04-41) [NBP3-33023] - Immunocytochemistry analysis of HeLa cells labeling TIMP-3 with Rabbit anti-TIMP-3 antibody (NBP3-33023) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TIMP-3 antibody (NBP3-33023) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33023_rabbit-timp-3-mab-psh04-41-2920241192391.jpg)