| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26395-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48439 | |||
| Azide and BSA Free | NBP3-11496 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26411-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21566-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21563-50ul | |||
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-45125 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45125, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-45125_rabbit-p16ink4a-cdkn2a-mab-30859-s17-chimeric-bsa-free-immunocytochemistry-immunofluorescence-222026035554.png "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-45125 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45125, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.")
![Western Blot: ERK1 Antibody [NBP1-78031] - Detection of ERK1 in whole cell lysates prepared from mouse brain (left), mouse kidney (center) and mouse intestine (right).](http://images.novusbio.com/fullsize/ERK1-Antibody-Western-Blot-NBP1-78031-img0001.jpg "Western Blot: ERK1 Antibody [NBP1-78031] - Detection of ERK1 in whole cell lysates prepared from mouse brain (left), mouse kidney (center) and mouse intestine (right).")
![Western Blot: Astrin Antibody [NB100-74639] - Detection of Human Astrin/MAP126 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74639 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74638 , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/Astrin-Antibody-Western-Blot-NB100-74639-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Astrin Antibody [NB100-74639] - Detection of Human Astrin/MAP126 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74639 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74638 , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![Immunoprecipitation: RSK1 [p Ser380] Antibody (1E11) [NBP3-26395] - Immunoprecipitating RSK1 [p Ser380] Antibody in Hela whole cell lysate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26395 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26395 (3ug) + Hela whole cell lysate (1mg). Lane 3: Hela whole cell lysate (20ug.)](http://images.novusbio.com/fullsize/nbp3-26395_rabbit-rsk1-p-ser-380-mab-1e11-26220241322153.jpg "Immunoprecipitation: RSK1 [p Ser380] Antibody (1E11) [NBP3-26395] - Immunoprecipitating RSK1 [p Ser380] Antibody in Hela whole cell lysate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26395 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26395 (3ug) + Hela whole cell lysate (1mg). Lane 3: Hela whole cell lysate (20ug.)")
![Western Blot: RSK1 [p Ser380] Antibody (1E11) [NBP3-26395] - Positive Western Blot detected in A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate. All lanes: RSK1 [p Ser380] Antibody at 1.75ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 KDa Observed band size: 90 KDa](http://images.novusbio.com/fullsize/nbp3-26395_rabbit-rsk1-p-ser-380-mab-1e11-28220241135165.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (SPM496) [NBP2-44841] - Human Tonsil stained with CD45 antibody (SPM496) [NBP2-44841].](http://images.novusbio.com/fullsize/CD45-Antibody-SPM496-Immunohistochemistry-Paraffin-NBP2-44841-img0001.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (SPM496) [NBP2-44841] - Human Tonsil stained with CD45 antibody (SPM496) [NBP2-44841].")
![Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-27220248471916.jpg "Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.")
![Flow Cytometry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Overlay Peak curve showing SH-SY5Y cells stained with Tau [p Ser199] Antibody (23E8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-282202414183638.jpg)
![Western Blot: Tau Antibody (S09-5E2) [NBP3-20020] - Western blot detection of Tau in Rat Brain cell using NBP3-20020 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S09-5E2-Western-Blot-NBP3-20020-img0001.jpg "Western Blot: Tau Antibody (S09-5E2) [NBP3-20020] - Western blot detection of Tau in Rat Brain cell using NBP3-20020 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Western Blot: Tau Antibody (S03-9I2) [NBP3-20059] - Western blot detection of Tau in Human Hippocampus lysates using NBP3-20059 (1:1000 diluted). Predicted band size: 79KDa. Observed band size: 50-80KDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S03-9I2-Western-Blot-NBP3-20059-img0001.jpg "Western Blot: Tau Antibody (S03-9I2) [NBP3-20059] - Western blot detection of Tau in Human Hippocampus lysates using NBP3-20059 (1:1000 diluted). Predicted band size: 79KDa. Observed band size: 50-80KDa.")
![Western Blot: AMPK alpha 2 [p Ser 491] Antibody (SR2078) [NBP3-21566] - Western blot analysis using NBP3-21566 in (1) 293T cell lysate treated with Lambda lysate; (2) 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21566_rabbit-ampk-alpha-2-p-ser-491-mab-sr2078-5720231624735.jpg "Western Blot: AMPK alpha 2 [p Ser 491] Antibody (SR2078) [NBP3-21566] - Western blot analysis using NBP3-21566 in (1) 293T cell lysate treated with Lambda lysate; (2) 293T cell lysate.")
![Western Blot: RSK1 [p Ser380] Antibody (S05-7F3) [NBP3-20065] - Western blot detection of RSK1 (phospho-Ser380) in CHO-K1 cell lysates using NBP3-20065 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 83kDa.](http://images.novusbio.com/fullsize/RSK1-p-Ser380-Antibody-S05-7F3-Western-Blot-NBP3-20065-img0001.jpg "Western Blot: RSK1 [p Ser380] Antibody (S05-7F3) [NBP3-20065] - Western blot detection of RSK1 (phospho-Ser380) in CHO-K1 cell lysates using NBP3-20065 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 83kDa.")
![Western Blot: Tau [p Ser202] Antibody (S01-5A7) [NBP3-20058] - Western blot detection of Tau in Rat Brain lysates using NBP3-20058 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-p-Ser202-Antibody-S01-5A7-Western-Blot-NBP3-20058-img0001.jpg "Western Blot: Tau [p Ser202] Antibody (S01-5A7) [NBP3-20058] - Western blot detection of Tau in Rat Brain lysates using NBP3-20058 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Western Blot: AMPK alpha 2 [p Ser 345] Antibody (SR2089) [NBP3-21563] - Western blot analysis using NBP3-21563 in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21563_rabbit-ampk-alpha-2-p-ser-345-mab-sr2089-5720231624719.jpg "Western Blot: AMPK alpha 2 [p Ser 345] Antibody (SR2089) [NBP3-21563] - Western blot analysis using NBP3-21563 in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate.")