| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25990 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25994 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | MAB85251-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP3-49667AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-49667AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-49667AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-49667AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-49667AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05536-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP3-49665AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-49665AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-49665AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-49665AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-49665AFP680 | |||
![Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg9]-Antibody-Chromatin-Immunoprecipitation-NBP2-44066-img0001.jpg "Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.")
![Western Blot: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - lane 1: HeLa Histone Prep Lysate. 2. NIH-3T3 Prep Lysate. Lane 3: C. elegans embryo lysate. Load: 30 Ug per lane. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg9]-Antibody-Western-Blot-NBP2-44066-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg9]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44066-img0002.jpg)
![Western Blot of Rabbit Anti-Histone H3 [Asym-dimethyl Arg9] Antibody Antibody. Lane 1: HeLa Histone Prep Lysate. 2. NIH-3T3 Prep Lysate. Lane 3: C. elegans embryo lysate. Load: 30 ug per lane. Primary antibody: Histone H3 [Asym-dimethyl Arg9] Antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.](http://images.novusbio.com/fullsize/nbp2-44066_rabbit-polyclonal-histone-h3-asym-dimethyl-arg9-antibody-245202310564613.jpg)
![Western Blot: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with Sodium Butyrate (+), using NBP2-61497 at 1 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys79-Antibody-RM156-Western-Blot-NBP2-61497-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with Sodium Butyrate (+), using NBP2-61497 at 1 ug/ml. Observed molecular weight is ~16 kDa.")
![<b>Biological Strategies Validation. </b>Multiplex Immunoassay: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP2-61497] - RM156 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.](http://images.novusbio.com/fullsize/nbp2-61497_rabbit-monoclonal-histone-h3-ac-lys79-antibody-rm156-multiplex-immunoassay-189202523415423.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using 5 ug NBP2-61497. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61497_rabbit-monoclonal-histone-h3-ac-lys79-antibody-rm156-chromatin-immunoprecipitation-18920252338177.png)
![ELISA: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - Sandwich ELISA against acetylated histone H3 using NBP2-61497 at 5 ug/ml as the capture antibody and NBP2-61547 at 1 ug/ml as the detection antibody.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys79-Antibody-RM156-ELISA-NBP2-61497-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP2-61497] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61497_rabbit-monoclonal-histone-h3-ac-lys79-antibody-rm156-immunocytochemistry-immunofluorescence-189202523374010.png)
![Western Blot: GAPDH Antibody (RM114) [NBP2-77402] - Western blot of A431 cells, using NBP2-77402, showed a band of GAPDH at the predicted MW (~36 kDa).](http://images.novusbio.com/fullsize/GAPDH-Antibody-RM114-Western-Blot-NBP2-77402-img0002.jpg "Western Blot: GAPDH Antibody (RM114) [NBP2-77402] - Western blot of A431 cells, using NBP2-77402, showed a band of GAPDH at the predicted MW (~36 kDa).")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (RM114) [NBP2-77402] - Immunocytochemical staining of HeLa cells, using NBP2-77402 (red). Nuclei have been labeled with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-77402_rabbit-monoclonal-gapdh-antibody-rm114-immunocytochemistry-immunofluorescence-199202502434.png)
![Western Blot: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with Sodium Butyrate, using NBP2-61536 at 0.25 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Western-Blot-NBP2-61536-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with Sodium Butyrate, using NBP2-61536 at 0.25 ug/ml. Observed molecular weight is ~17 kDa.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Immunohistochemistry staining of HepG2 cells using NBP2-61536.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Immunohistochemistry-NBP2-61536-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP2-61536] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61536_rabbit-monoclonal-histone-h3-ac-lys9-antibody-rm161-immunocytochemistry-immunofluorescence-1892025233714.png)
![ELISA: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Sandwich ELISA using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using NBP2-61547 at 1 ug/ml as the capture antibody and NBP2-61536 at 1 ug/ml as the detection antibody.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-ELISA-NBP2-61536-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (919022) [Unconjugated] [MAB85251] - Lamin B1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB85251) at 1.7 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-919022-Unconjugated-Immunohistochemistry-Paraffin-MAB85251-img0001.jpg "Immunohistochemistry-Paraffin: Lamin B1 Antibody (919022) [Unconjugated] [MAB85251] - Lamin B1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB85251) at 1.7 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei.")
![Western Blot: HSP60 Antibody [NBP3-05536] - Western blot analysis of different cell lysates using HSP60 Antibody at a dilution of 1:20,000, in green. [1] protein standard (red), [2] SH-SY5Y cells, [3] HeLa cells. The strong single strong band corresponds to the HSP60 protein with an apparent SDS-PAGE molecular weight of 60kDa.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP3-05536-img0002.jpg "Western Blot: HSP60 Antibody [NBP3-05536] - Western blot analysis of different cell lysates using HSP60 Antibody at a dilution of 1:20,000, in green. [1] protein standard (red), [2] SH-SY5Y cells, [3] HeLa cells. The strong single strong band corresponds to the HSP60 protein with an apparent SDS-PAGE molecular weight of 60kDa.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP3-05536] - Immunofluorescent analysis of of HeLa cells stained with HSP60 Antibody at a dilution of 1:5,000 in green and costained with mouse mAb to actin at a dilution of 1:500 in red. Blue is DAPI staining of nuclear DNA. The HSP60 antibody produces strong and specific staining of mitochondria, while the actin antibody labels the submembranous cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05536-img0001.jpg)
![Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Western-Blot-NBP2-74583-img0005.jpg "Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).")
![Immunocytochemistry/Immunofluorescence: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Transferrin.](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74583-img0004.jpg)
![Flow Cytometry: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Transferrin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74583-img0003.jpg)
![Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Western-Blot-NBP2-74583-img0001.jpg)
![Western Blot: Transferrin Antibody (OTI5G2) - Azide and BSA Free [NBP2-74583] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF .](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Azide-and-BSA-Free-Western-Blot-NBP2-74583-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated 500ng/mL TSA for 4 hours and either Histone H3 (NBP3-32429) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-26820241525100.jpg "Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated 500ng/mL TSA for 4 hours and either Histone H3 (NBP3-32429) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Dot Blot: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Dot blot analysis of Histone H3 on different proteins with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys4) (negative) Lane 3: Di-Methyl-Histone H3 (Lys4) (negative) Lane 4: Tri-Methyl-Histone H3 (Lys4) (negative) Lane 5: Acetyl-Histone H3 (Lys4) (positive) Lane 6: Acetyl-Histone H3 (Lys9) (negative) Lane 7: Acetyl-Histone H3 (Lys27) (negative) Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 50 seconds.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-28820241459250.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-2920241192347.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-3920248453547.jpg)