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![Western Blot: Cyclophilin B Antibody (M019) [NBP3-23271] - Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (NBP3-23271) at 1:500.](http://images.novusbio.com/fullsize/nbp3-23271_mouse-cyclophilin-b-mab-m019-199202318202910.jpg "Western Blot: Cyclophilin B Antibody (M019) [NBP3-23271] - Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (NBP3-23271) at 1:500.")
![Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody (M019) [NBP3-23271] - Immunocytochemical labeling of cyclophilin B in methanol:acetone (1:1) fixed human A549 lung cancer cells. The cells were labeled with mouse monoclonal anti-cyclophilin B (NBP3-23271). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23271_mouse-cyclophilin-b-mab-m019-18920231735529.jpg)
![Western Blot: beta-Actin Antibody (M008) [NBP3-23324] - Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-beta-Actin (NBP3-23324) at 1:1000 (lanes 1-6).](http://images.novusbio.com/fullsize/nbp3-23324_mouse-beta-actin-mab-m008-19920231820297.jpg "Western Blot: beta-Actin Antibody (M008) [NBP3-23324] - Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-beta-Actin (NBP3-23324) at 1:1000 (lanes 1-6).")
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (M008) [NBP3-23324] - Immunocytochemical labeling of beta-Actin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-beta-Actin (clone M008). The antibody was detected using goat anti-mouse DyLight (R) 594.](http://images.novusbio.com/fullsize/nbp3-23324_mouse-beta-actin-mab-m008-1992023141019.jpg)
![Western Blot: Cyclophilin B Antibody [NBP1-62356] - HepG2 cell lysate, antibody titration 1.25ug/ml. Gel concentration 12%](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP1-62356-img0004.jpg "Western Blot: Cyclophilin B Antibody [NBP1-62356] - HepG2 cell lysate, antibody titration 1.25ug/ml. Gel concentration 12%")
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-62356] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-62356-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody (C.15200182) [NBP2-59296] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me2 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys36-Antibody-C-15200182-Chromatin-Immunoprecipitation-NBP2-59296-img0002.jpg "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody (C.15200182) [NBP2-59296] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me2 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![ELISA: Histone H3 [Dimethyl Lys36] Antibody (C.15200182) [NBP2-59296] - Cross reactivity of the antibody directed against H3K36me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K36me2. The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys36-Antibody-C-15200182-ELISA-NBP2-59296-img0001.jpg)
![Western Blot: Histone H3 [Monomethyl Arg2] Antibody (3Y5F9) [NBP3-15834] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Arg2-Antibody-3Y5F9-Western-Blot-NBP3-15834-img0001.jpg "Western Blot: Histone H3 [Monomethyl Arg2] Antibody (3Y5F9) [NBP3-15834] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Dot Blot: Histone H3 [Monomethyl Arg2] Antibody (3Y5F9) [Histone H3] - Dot-blot analysis of all sorts of peptides using Histone H3 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-15834_rabbit-histone-h3-monomethyl-arg-2-mab-3y5f9-52202517273515.jpg)
![Western Blot: Cyclophilin B Antibody [NBP2-98906] - Lane A: Hela whole cell lysateLane B: A431 whole cell lysateLane C: HepG2 whole cell lysateLane D: 293 whole cell lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP2-98906-img0006.jpg "Western Blot: Cyclophilin B Antibody [NBP2-98906] - Lane A: Hela whole cell lysateLane B: A431 whole cell lysateLane C: HepG2 whole cell lysateLane D: 293 whole cell lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)")
![Western Blot: Cyclophilin B Antibody [NBP2-98906] - Anti-Cyclophilin B rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 19 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP2-98906-img0004.jpg)
![Immunoprecipitation: Cyclophilin B Antibody [NBP2-98906] - Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-PPIB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-PPIB rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size: 19 kDa](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunoprecipitation-NBP2-98906-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Transferrin Antibody (TW4.20) [NBP2-81094] - Immunofluorescence staining of fixed HepG2 cells with Transferrin Antibody (TW4.20) [NBP2-81094]. Immunofluorescence analysis of paraformaldehyde fixed HpeG2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of TW4.20 [NBP2-81094] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing diffuse cytoplasmic staining, likely from protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81094, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Transferrin-Antibody-TW4-20-Immunocytochemistry-Immunofluorescence-NBP2-81094-img0001.jpg "Immunocytochemistry/Immunofluorescence: Transferrin Antibody (TW4.20) [NBP2-81094] - Immunofluorescence staining of fixed HepG2 cells with Transferrin Antibody (TW4.20) [NBP2-81094]. Immunofluorescence analysis of paraformaldehyde fixed HpeG2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of TW4.20 [NBP2-81094] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing diffuse cytoplasmic staining, likely from protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81094, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![SDS-Page: Recombinant Human beta-Actin Protein [H00000060-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-beta-Actin-Protein-SDS-Page-H00000060-P01-img0002.jpg "SDS-Page: Recombinant Human beta-Actin Protein [H00000060-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody (RM141) - Azide and BSA Free [NBP3-25987] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM141 at 0. 5 ug/mL, showed a band of histone H3 dimethylated at Lysine 36 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25987_rabbit-histone-h3-mab-rm141-azide-and-bsa-free-24620241332469.png "Western Blot: Histone H3 [Dimethyl Lys36] Antibody (RM141) - Azide and BSA Free [NBP3-25987] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM141 at 0. 5 ug/mL, showed a band of histone H3 dimethylated at Lysine 36 in HeLa cells.")
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys36] Antibody (RM141) - Azide and BSA Free [NBP3-25987] - RM141 specifically reacts to Histone H3 dimethylated at Lysine 36 (K36me2). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1), trimethylated Lysine 36 (K36me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25987_rabbit-histone-h3-mab-rm141-azide-and-bsa-free-2462024132613.png)