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![Western Blot: ACADM Antibody [NB100-1121] - Analysis of ACADM in Human Heart lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 0.05 ug/mL. Primary incubated for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACADM-Antibody-Western-Blot-NB100-1121-img0007.jpg "Western Blot: ACADM Antibody [NB100-1121] - Analysis of ACADM in Human Heart lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 0.05 ug/mL. Primary incubated for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: ACADM Antibody [NB100-1121] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NB100-1121-img0008.jpg)
![Immunohistochemistry-Paraffin: ACADM Antibody [NB100-1121] - Staining of paraffin embedded human liver using this antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NB100-1121-img0006.jpg)
![Western Blot: OSBP1 Antibody [NB100-1500] - Analysis of Human Placenta lysate (35ug protein in RIPA buffer) using NB100-1500 (0.1ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/OSBP1-Antibody-Western-Blot-NB100-1500-img0002.jpg "Western Blot: OSBP1 Antibody [NB100-1500] - Analysis of Human Placenta lysate (35ug protein in RIPA buffer) using NB100-1500 (0.1ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466] - Western blot analysis of nuclear HeLa cell lysate (A) + peptide (B) and nuclear K562 cell lysate (C) + peptide (D). 35 ug protein in RIPA buffer. Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-Western-Blot-NB100-1466-img0001.jpg "Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466] - Western blot analysis of nuclear HeLa cell lysate (A) + peptide (B) and nuclear K562 cell lysate (C) + peptide (D). 35 ug protein in RIPA buffer. Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody [NB100-1466] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1466-img0002.jpg)
![Flow Cytometry: RXR alpha/NR2B1 Antibody [NB100-1466] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-Flow-Cytometry-NB100-1466-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-1464] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1464-img0002.jpg "Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-1464] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: LXR beta/NR1H2 Antibody [NB100-1464] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Flow-Cytometry-NB100-1464-img0001.jpg)
![Western Blot: COMT Antibody [NB100-41387] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NB100-41387-img0007.jpg "Western Blot: COMT Antibody [NB100-41387] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0006.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Cerebellum. Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0004.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0005.jpg)
![Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-Western-Blot-NB100-1503-img0008.jpg "Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - Analysis of LCN2 in human xenograft tumor. Image courtesy of product review submitted by Yi-Ling Huang.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-Immunohistochemistry-Paraffin-NB100-1503-img0006.jpg)
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-Immunohistochemistry-Paraffin-NB100-1503-img0005.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - HEK293 overexpressing ALDH1A1 and probed with NB100-2563 (mock transfection in first lane).](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NB100-2563-img0005.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - HEK293 overexpressing ALDH1A1 and probed with NB100-2563 (mock transfection in first lane).")
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NB100-2563-img0006.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - (1ug/ml) Analysis of Human Liver Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NB100-2563-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06014-img0009.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP1-06014] - Staining of paraffin embedded Human Cerebellum AIF-1/Iba1 Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP1-06014-img0007.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP1-06014] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) with AIF-1:Iba1 Antibody, followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP1-06014-img0008.jpg)
![Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody (M01), clone 3E4.Lane 1: PCK1 transfected lysate(69.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Western-Blot-H00005105-M01-img0008.jpg "Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody (M01), clone 3E4.Lane 1: PCK1 transfected lysate(69.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of monoclonal antibody to PCK1 on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Immunocytochemistry-Immunofluorescence-H00005105-M01-img0005.jpg)
![Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - PCK1 monoclonal antibody (M01), clone 3E4 Analysis of PCK1 expression in HepG2.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Western-Blot-H00005105-M01-img0007.jpg)
![Immunoprecipitation: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 transfected lysate using anti-PCK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Immunoprecipitation-H00005105-M01-img0006.jpg)
![Western Blot: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Analysis of NR1H2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1E1-Western-Blot-H00007376-M04-img0008.jpg "Western Blot: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Analysis of NR1H2 expression in PC-12 (Cat # L012V1).")
![Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Analysis of monoclonal antibody to NR1H2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1E1-Immunocytochemistry-Immunofluorescence-H00007376-M04-img0006.jpg)
![Western Blot: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Analysis of NR1H2 expression in IMR-32 (Cat # L008V1).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1E1-Western-Blot-H00007376-M04-img0009.jpg)
![Sandwich ELISA: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Detection limit for recombinant GST tagged NR1H2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1E1-Sandwich-ELISA-H00007376-M04-img0007.jpg)
![Western Blot: VDAC2 Antibody (3D2) [H00007417-M01] - VDAC2 monoclonal antibody (M01), clone 3D2. Analysis of VDAC2 expression in rat brain.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Western-Blot-H00007417-M01-img0006.jpg "Western Blot: VDAC2 Antibody (3D2) [H00007417-M01] - VDAC2 monoclonal antibody (M01), clone 3D2. Analysis of VDAC2 expression in rat brain.")
![Immunocytochemistry/Immunofluorescence: VDAC2 Antibody (3D2) [H00007417-M01] - Analysis of monoclonal antibody to VDAC2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Immunocytochemistry-Immunofluorescence-H00007417-M01-img0003.jpg)
![Western Blot: VDAC2 Antibody (3D2) [H00007417-M01] - VDAC2 monoclonal antibody (M01), clone 3D2. Analysis of VDAC2 expression in K-562.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Western-Blot-H00007417-M01-img0005.jpg)
![Western Blot: VDAC2 Antibody (3D2) [H00007417-M01] - Analysis of VDAC2 expression in transfected 293T cell line by VDAC2 monoclonal antibody (M01), clone 3D2.Lane 1: VDAC2 transfected lysate(31.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Western-Blot-H00007417-M01-img0007.jpg)
![Sandwich ELISA: VDAC2 Antibody (3D2) [H00007417-M01] - Detection limit for recombinant GST tagged VDAC2 is 10 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Sandwich-ELISA-H00007417-M01-img0004.jpg)
![Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-288202414191525.jpg "Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: xCT Antibody (A7C6-R) [NBP3-33104] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT antibody (NBP3-33104) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33104) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-298202413585586.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody (A7C6-R) [NBP3-33104] - Immunocytochemistry analysis of HT-29 cells labeling xCT with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-2920241114813.jpg)
![Flow Cytometry: xCT Antibody (A7C6-R) [NBP3-33104] - Flow cytometric analysis of A549 cells labeling xCT. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33104, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-29202416372152.jpg)