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![Western Blot: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI 3-Kinase p85 alpha (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).](http://images.novusbio.com/fullsize/nbp3-23296_mouse-pi-3-kinase-p85-alpha-mab-m253-199202312523621.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI 3-Kinase p85 alpha (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Immunocytochemical labeling of PI 3-Kinase p85 alpha in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI 3-Kinase p85 alpha (C-terminal region) antibody (NBP3-23296), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23296_mouse-pi-3-kinase-p85-alpha-mab-m253-199202318202914.jpg)
![Immunoprecipitation: ADK Antibody [NBP2-32189] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Adenosine Kinase antibody NBP2-32189 used for IP at 6 ug per reaction. Adenosine Kinase was also immunoprecipitated by rabbit anti-Adenosine Kinase antibody BL15848. For blotting immunoprecipitated Adenosine Kinase, NBP2-32189 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunoprecipitation-NBP2-32189-img0003.jpg "Immunoprecipitation: ADK Antibody [NBP2-32189] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Adenosine Kinase antibody NBP2-32189 used for IP at 6 ug per reaction. Adenosine Kinase was also immunoprecipitated by rabbit anti-Adenosine Kinase antibody BL15848. For blotting immunoprecipitated Adenosine Kinase, NBP2-32189 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: ADK Antibody [NBP2-32189] - Wholecell lysate (50 ug) from HEK293T, LNCaP, and MCF-7 cellsprepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-ADK antibody usedfor WB at 0.4 ug/ml. Detection: Chemiluminescence withan exposure time of 10 seconds.](http://images.novusbio.com/fullsize/antibody/nbp2-32189_rabbit-polyclonal-adk-antibody-western-blot-2922024103050..jpg)
![Western Blot: ADK Antibody [NBP2-32189] - : Wholecell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1,and BW5147.3 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-ADK antibody used for WB at 0.4 ug/ml. Detection:Chemiluminescence with an exposure time of 10 seconds](http://images.novusbio.com/fullsize/antibody/nbp2-32189_rabbit-polyclonal-adk-antibody-western-blot-292202410329..jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFK-1, NBP2-32170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32170-img0003.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFK-1, NBP2-32170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for WB at 0.1ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32170-img0004.jpg)
![Western Blot: ADK Antibody [NBP1-31802] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP1-31802-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ADK Antibody [NBP1-31802] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP1-31802] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Adenosine kinase protein stained by Adenosine kinase antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31802-img0004.jpg)
![Western Blot: ADK Antibody [NBP1-31802] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP1-31802-img0003.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (CA9/781) [NBP2-45237] - Human Renal Cell Carcinoma stained with CAIX Monoclonal Antibody (CA9/781).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-CA9-781-Immunohistochemistry-Paraffin-NBP2-45237-img0001.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (CA9/781) [NBP2-45237] - Human Renal Cell Carcinoma stained with CAIX Monoclonal Antibody (CA9/781).")
![Western Blot: Carbonic Anhydrase IX/CA9 Antibody (CA9/781) [NBP2-45237] - Analysis of HCT116 Cell Lysate using CAIX Monoclonal Antibody (CA9/781). As indicated by the arrow, a specific band was detected at a molecular weight of approximately 50 kDa.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-CA9-781-Western-Blot-NBP2-45237-img0002.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM487) [NBP2-34363] - FFPE human renal cell carcinoma stained with RCC MAb (SPM487). Note cytoplasmic & cell surface staining of tumor cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-SPM487-Immunohistochemistry-Paraffin-NBP2-34363-img0003.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM487) [NBP2-34363] - FFPE human renal cell carcinoma stained with RCC MAb (SPM487). Note cytoplasmic & cell surface staining of tumor cells.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM314) [NBP2-34360] - FFPE human renal cell carcinoma stained with RCC MAb (SPM314). Note cytoplasmic & cell surface staining of tumor cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-SPM314-Immunohistochemistry-Paraffin-NBP2-34360-img0003.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM314) [NBP2-34360] - FFPE human renal cell carcinoma stained with RCC MAb (SPM314). Note cytoplasmic & cell surface staining of tumor cells.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM487) - Azide and BSA Free [NBP2-34411] - FFPE human renal cell carcinoma stained with RCC MAb (SPM487). Note cytoplasmic & cell surface staining of tumor cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-SPM487-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34411-img0001.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM487) - Azide and BSA Free [NBP2-34411] - FFPE human renal cell carcinoma stained with RCC MAb (SPM487). Note cytoplasmic & cell surface staining of tumor cells.")