| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90272-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89345-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99732-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99029-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22155-50ul | |||
![Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557] - Western blot analysis of extracts of various cell lines, using PER2 Rabbit mAb (NBP3-16557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PER2-Antibody-2K9Y0-Western-Blot-NBP3-16557-img0003.jpg "Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557] - Western blot analysis of extracts of various cell lines, using PER2 Rabbit mAb (NBP3-16557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Chromatin Immunoprecipitation: PER2 Antibody (2K9Y0) [NBP3-16557] - Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using PER2 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16557_rabbit-per2-mab-2k9y0-5220251255595.jpg)
![Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Western-Blot-NBP2-90272-img0001.jpg "Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa")
![Immunoprecipitation: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate2 uL anti-AIF1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AIF1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Immunoprecipitation-NBP2-90272-img0002.jpg)
![Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-002-Flow-Cytometry-NBP2-89345-img0001.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Leptin/OB Antibody (002) [NBP2-89345] - Staining of LEP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89345 (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm .](http://images.novusbio.com/fullsize/nbp2-89345_rabbit-monoclonal-leptin-ob-antibody-002-23520251129371.png)
![Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP2-99732] - Immunochemical staining of human CD39L4/ENTPD5 in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-Immunohistochemistry-Paraffin-NBP2-99732-img0002.jpg "Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP2-99732] - Immunochemical staining of human CD39L4/ENTPD5 in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.")
![Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP2-99732] - Immunochemical staining of human CD39L4/ENTPD5 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-Immunohistochemistry-Paraffin-NBP2-99732-img0001.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Western-Blot-NBP2-99029-img0002.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Leukotriene A4 Hydrolase/LTA4H was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Immunoprecipitation-NBP2-99029-img0001.jpg)
![SDS-Page: Human Leptin/OB Protein [NBP2-34934]](http://images.novusbio.com/fullsize/Human-Leptin-OB-Protein-SDS-Page-NBP2-34934-img0001.jpg "SDS-Page: Human Leptin/OB Protein [NBP2-34934]")
![Western Blot: PER2 Antibody (SR1141) [NBP3-22155] - Western blot analysis using NBP3-22155 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22155_rabbit-per2-mab-sr1141-57202317215177.jpg "Western Blot: PER2 Antibody (SR1141) [NBP3-22155] - Western blot analysis using NBP3-22155 in HeLa cell lysate.")