| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55509-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-158SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35134-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92806-0.02ml | |||
![Western Blot: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Knockdown-Validated-NBP2-55509-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: HSP90 beta Antibody [NBP2-55509] - Analysis using Anti-HSP90AB1 antibody NBP2-55509 (A) shows similar pattern to independent antibody NBP2-68978 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Western-Blot-NBP2-55509-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP2-55509] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55509-img0001.jpg)
![Western Blot: GABARAP Antibody [NB300-158] - Analysis of GABARAP in brain lysate using this antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GABARAP-Antibody-Western-Blot-NB300-158-img0002.jpg "Western Blot: GABARAP Antibody [NB300-158] - Analysis of GABARAP in brain lysate using this antibody at 1:500 dilution.")
![Western Blot: GRASP55 Antibody [NBP3-35134] - Western blot analysis of various lysates using GRASP55 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35134_rabbit-grasp55-pab-912202415121322.jpg "Western Blot: GRASP55 Antibody [NBP3-35134] - Western blot analysis of various lysates using GRASP55 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP3-35134] - Immunofluorescence analysis of U-2 OS cells using GRASP55 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35134_rabbit-grasp55-pab-31120251704795.jpg)
![Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP3-35134] - Immunofluorescence analysis of L929 cells using GRASP55 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35134_rabbit-grasp55-pab-3112025173179.jpg)
![Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP3-35134] - Immunofluorescence analysis of H9c2 cells using GRASP55 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35134_rabbit-grasp55-pab-311202517706.jpg)
![Western Blot: Transferrin Antibody [NB600-931] - Lane 1: molecular weight. Lane 2: Rabbit Antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight (TM) 649 conjugated (green), Goat IgG (H&L) Antibody DyLight (TM) 549 conjugated (red), Mouse IgG (H&L) Antibody DyLight (TM) 488 conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer for 1hr at 4C.](http://images.novusbio.com/fullsize/Transferrin Antibody-Western Blot-NB600-931-img0004.jpg "Western Blot: Transferrin Antibody [NB600-931] - Lane 1: molecular weight. Lane 2: Rabbit Antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight (TM) 649 conjugated (green), Goat IgG (H&L) Antibody DyLight (TM) 549 conjugated (red), Mouse IgG (H&L) Antibody DyLight (TM) 488 conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer for 1hr at 4C.")
![Western Blot: Transferrin Antibody [NB600-931] - Lane 1: molecular weight. Lane 2: anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NB600-931-img0002.jpg)
![Western Blot: Transferrin Antibody [NB600-931] - Lane 1: Human Transferrin. Lane 2: None.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NB600-931-img0003.jpg)
![Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Analysis of ST6GAL1 expression in transfected 293T cell line by ST6GAL1 polyclonal antibody. A: Non-transfected lysate. B: ST6GAL1 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/ST6-Gal-Sialyltransferase-1-ST6GAL1-CD75-Antibody-Western-Blot-NBP2-15817-img0004.jpg "Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Analysis of ST6GAL1 expression in transfected 293T cell line by ST6GAL1 polyclonal antibody. A: Non-transfected lysate. B: ST6GAL1 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Human hepatocellular carcinoma. CD75 antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/ST6-Gal-Sialyltransferase-1-ST6GAL1-CD75-Antibody-Immunohistochemistry-Paraffin-NBP2-15817-img0003.jpg)
![Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Analysis of paraffin-embedded OVCA xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ST6-Gal-Sialyltransferase-1-ST6GAL1-CD75-Antibody-Immunohistochemistry-Paraffin-NBP2-15817-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD75 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-15817_rabbit-polyclonal-st6-gal-sialyltransferase-1-st6gal1-cd75-antibody-1610202419414585.jpg)
![Western Blot: MGAT4A Antibody [NBP2-17285] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/MGAT4A-Antibody-Western-Blot-NBP2-17285-img0001.jpg "Western Blot: MGAT4A Antibody [NBP2-17285] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: MGAT4A Antibody [NBP2-17285] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MGAT4A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17285-img0003.jpg)
![Immunohistochemistry-Paraffin: MGAT4A Antibody [NBP2-17285] - Immunohistochemical analysis of paraffin-embedded H358 xenograft, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/MGAT4A-Antibody-Immunohistochemistry-Paraffin-NBP2-17285-img0002.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Western-Blot-NBP1-22410-img0005.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Immunocytochemistry-Immunofluorescence-NBP1-22410-img0006.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Western-Blot-NBP1-04346-img0007.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Immunocytochemistry-Immunofluorescence-NBP1-04346-img0006.jpg)
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). Thesecondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Flow-Cytometry-NBP1-04346-img0008.jpg)
![SDS-Page: Recombinant Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Protein [NBP2-52038]](http://images.novusbio.com/fullsize/Recombinant-Human-ST6-Gal-Sialyltransferase-1-ST6GAL1-CD75-Protein-SDS-Page-NBP2-52038-img0002.jpg "SDS-Page: Recombinant Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Protein [NBP2-52038]")
![Immunohistochemistry;COPB2 Antibody[NBP3-30571]Detection of mouse COPB2 by immunohistochemistry. Samples: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30571_rabbit-copb2-pab-782024936116.jpg "Immunohistochemistry;COPB2 Antibody[NBP3-30571]Detection of mouse COPB2 by immunohistochemistry. Samples: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1:1,000 . Detection: DAB")
![Immunohistochemistry;COPB2 Antibody[NBP3-30548]Detection of human COPB2 by immunohistochemistry. Samples: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30571_rabbit-copb2-pab-78202493613.jpg)
![Immunoprecipitation:COPB2 AntibodyNBP3-30571] -Detection of human COPB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COPB2 antibody (NBP3-30571 lot 2) used for IP at 6 µg per reaction. COPB2 was also immunoprecipitated by a previous lot of this antibody (NBP3-30571 lot 1) and a second.inst a different epitope of COPB2 (A304-522A). For blotting immunoprecipitated COPB2, NBP3-30571 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp3-30571_rabbit-copb2-pab-301220242041579.jpg)
![Western Blot: ATG9B Antibody [NBP2-92806] - Western blot analysis of extracts of HUH-7 cells, using ATG9B antibody (NBP2-92806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ATG9B-Antibody-Western-Blot-NBP2-92806-img0001.jpg "Western Blot: ATG9B Antibody [NBP2-92806] - Western blot analysis of extracts of HUH-7 cells, using ATG9B antibody (NBP2-92806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: ATG9B Antibody [NBP2-92806] - Western blot analysis of extracts of various cell lines, using ATG9B antibody (NBP2-92806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ATG9B Antibody-Western Blot-NBP2-92806-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - Azide and BSA Free [NBP2-92806] - Immunofluorescence analysis of human placenta cells using ATG9B Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92806_rabbit-polyclonal-atg9b-antibody-4220251430417.jpg%3Ft=1698886526)