| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89207-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99049-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12777-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98742-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89318-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-23531-20ul | |||
![Immunohistochemistry-Paraffin: PAK6 Antibody [NLS6942] - Human Lung, Non-Small Cell Carcinoma. formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PAK6-Antibody-Immunohistochemistry-Paraffin-NLS6942-img0003.jpg "Immunohistochemistry-Paraffin: PAK6 Antibody [NLS6942] - Human Lung, Non-Small Cell Carcinoma. formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: PAK6 Antibody [NLS6942] - Analysis of anti-PAK6 antibody with human brain, neurons and glia at 3.5 ug/ml.](http://images.novusbio.com/fullsize/PAK6-Antibody-Immunohistochemistry-Paraffin-NLS6942-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89207_rabbit-monoclonal-4ebp1-antibody-007-23520251154563.png "Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.")
![Immunohistochemistry: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-Immunohistochemistry-NBP2-99049-img0003.jpg "Immunohistochemistry: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK alpha in human breast carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99049-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: IKK alpha Antibody [NBP2-99049] - Immunofluorescence staining of IKK alpha in HCT116 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99049 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-99049_rabbit-polyclonal-ikk-alpha-antibody-2352025166478.png)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg "Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western blot analysis on different lysates with NBP3-32688 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32688 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_-western-blot-639204590074505803.jpg)
![ELISA: Human Rad51 ELISA Kit (Colorimetric) [NBP3-40314] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40314_human-rad51-elisa-kit-colorimetric-2110202415355081.png "ELISA: Human Rad51 ELISA Kit (Colorimetric) [NBP3-40314] - Standard Curve Reference")
![Immunohistochemistry-Paraffin: FANCC Antibody [NBP3-12777] - Staining of human FANCC in human brain with rabbit polyclonal antibody (1:2000).](http://images.novusbio.com/fullsize/FANCC-Antibody-Immunohistochemistry-Paraffin-NBP3-12777-img0003.jpg "Immunohistochemistry-Paraffin: FANCC Antibody [NBP3-12777] - Staining of human FANCC in human brain with rabbit polyclonal antibody (1:2000).")
![Immunohistochemistry-Paraffin: FANCC Antibody [NBP3-12777] - Staining of human FANCC in human pancreas with rabbit polyclonal antibody (1:2000).](http://images.novusbio.com/fullsize/FANCC-Antibody-Immunohistochemistry-Paraffin-NBP3-12777-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FANCC Antibody [NBP3-12777] - Staining of FANCC in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with NBP3-12777 antibody (1:1000)(Figure A), incubated with NBP3-12777 antibody and antigen (Figure B) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp3-12777_rabbit-polyclonal-fancc-antibody-23520252135160.png)
![Immunohistochemistry: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-DNA-PKcs antibody (NBP3-32272) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32272) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32272_mouse-dna-pkcs-mab-2-b3-r-298202414333675.jpg "Immunohistochemistry: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-DNA-PKcs antibody (NBP3-32272) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32272) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Immunocytochemistry analysis of HepG2 cells labeling DNA-PKcs with Rabbit anti-DNA-PKcs antibody (NBP3-32272) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DNA-PKcs antibody (NBP3-32272) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32272_mouse-dna-pkcs-mab-2-b3-r-29202410334012.jpg)
![Western blot analysis on different lysates with NBP3-32272 at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lane 6: HT-29 cell lysate Lane 7: A549 cell lysate Lane 8: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 469 kDa Observed band size: 469 kDa Exposure time: 23 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32272 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32272_-western-blot-639204590077132238.jpg)
![Immunohistochemistry: DNA Polymerase delta, catalytic subunit Antibody (JE35-73) [NBP3-32838] - Immunohistochemical analysis of paraffin-embedded <br/> rat testis tissue with Rabbit anti-DNA Polymerase delta, catalytic subunit antibody (NBP3-32838) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32838) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32838_rabbit-dna-polymerase-delta-catalytic-subunit-mab-je35-73-298202414115437.jpg "Immunohistochemistry: DNA Polymerase delta, catalytic subunit Antibody (JE35-73) [NBP3-32838] - Immunohistochemical analysis of paraffin-embedded <br/> rat testis tissue with Rabbit anti-DNA Polymerase delta, catalytic subunit antibody (NBP3-32838) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32838) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32838 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: PC-3M cell lysate (20 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Lane 8: Rat testis tissue lysate (40 µg/Lane) Lane 9: SW480 cell lysate (20 µg/Lane) Predicted band size: 124 kDa Observed band size: 124 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32838 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32838_-western-blot-639204595239342171.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-98742] - Immunochemical staining of human GADD153/CHOP in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-Paraffin-NBP2-98742-img0002.jpg "Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-98742] - Immunochemical staining of human GADD153/CHOP in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunocytochemistry/Immunofluorescence: GADD153/CHOP Antibody [NBP2-98742] - Immunofluorescence staining of GADD153/CHOP in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98742 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98742_rabbit-polyclonal-gadd153-chop-antibody-235202521525024.png)
![Immunocytochemistry/Immunofluorescence: MSH6 Antibody (021) [NBP2-89318] - Staining of MSH6 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MSH6 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/MSH6-Antibody-021-Immunocytochemistry-Immunofluorescence-NBP2-89318-img0004.jpg "Immunocytochemistry/Immunofluorescence: MSH6 Antibody (021) [NBP2-89318] - Staining of MSH6 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MSH6 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: MSH6 Antibody (021) [NBP2-89318] - Staining of human MSH6 in human sigmoid colon cancer with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MSH6-Antibody-021-Immunohistochemistry-Paraffin-NBP2-89318-img0003.jpg)
![Immunohistochemistry-Paraffin: MSH6 Antibody (021) [NBP2-89318] - Staining of human MSH6 in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MSH6-Antibody-021-Immunohistochemistry-Paraffin-NBP2-89318-img0001.jpg)
![Immunohistochemistry-Paraffin: MSH6 Antibody (021) [NBP2-89318] - Staining of human MSH6 in human tonsil with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MSH6-Antibody-021-Immunohistochemistry-Paraffin-NBP2-89318-img0002.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Western blot analysis of extracts from serum-starved HEK293, treated with 20% FBS (30 min), using NBP3-23531 at dilution of 1:1000, 1:5000 and 1:25000.](http://images.novusbio.com/fullsize/nbp3-23531_rabbit-ribosomal-protein-s6-rps6-mab-0018-azide-bsa-free-235202511354146.png "Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Western blot analysis of extracts from serum-starved HEK293, treated with 20% FBS (30 min), using NBP3-23531 at dilution of 1:1000, 1:5000 and 1:25000.")
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Western blot analysis of extracts from serum-starved , untreated (line A); treated with FBS (20%, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using NBP3-23531 at 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp3-23531_rabbit-ribosomal-protein-s6-rps6-mab-0018-azide-bsa-free-235202521451756.png)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using NBP3-23531 at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-23531_rabbit-ribosomal-protein-s6-rps6-mab-0018-azide-bsa-free-235202514492317.png)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser240, p Ser244] Antibody (0018) [NBP3-23531] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with FBS (20%, 30min) (line B); treated with FBS and -phosphatase (line C) using NBP3-23531 at 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp3-23531_rabbit-ribosomal-protein-s6-rps6-mab-0018-azide-bsa-free-235202512205527.png)
![Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15220001) [NBP2-59178] - Hydroxymethylated DNA IP (hMeDIP) assays were performed using the antibody against 5-hydroxymethylcytosine, 5-hmC, 5-mC and cytosine DNA standards and Rat IgG. DNA fragments are 300-500 bp. 1 ug of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP'd material has been analyzed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control, with Rat IgG as negative control (0.06%, almost no recovery), with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control, with Rat IgG as negative control (0.03%, almost no recovery), with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control, with Rat IgG as negative control (0.04%, almost no recovery), with 5-hmC (97.60% recovery, almost full recovery).](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15220001-Methylated-DNA-Immunoprecipitation-NBP2-59178-img0002.jpg "Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15220001) [NBP2-59178] - Hydroxymethylated DNA IP (hMeDIP) assays were performed using the antibody against 5-hydroxymethylcytosine, 5-hmC, 5-mC and cytosine DNA standards and Rat IgG. DNA fragments are 300-500 bp. 1 ug of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP'd material has been analyzed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control, with Rat IgG as negative control (0.06%, almost no recovery), with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control, with Rat IgG as negative control (0.03%, almost no recovery), with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control, with Rat IgG as negative control (0.04%, almost no recovery), with 5-hmC (97.60% recovery, almost full recovery).")
![ELISA: 5-hmC Antibody (C.15220001) [NBP2-59178] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC in antigen coated wells. The antigen used was a 5-hmC base coupled to KHL. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15220001-ELISA-NBP2-59178-img0001.jpg)
![Dot Blot: 5-hmC Antibody (C.15220001) [NBP2-59178] - (A): Approximately 200 ng, equivalent 10 pmol of C-bases, of the hmC (1), mC (2) and C (3) PCR controls were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 5-hmC Antibody (C.15220001) followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.](http://images.novusbio.com/fullsize/5-hmC-Antibody-C-15220001-Dot-Blot-NBP2-59178-img0004.jpg)
![Dot Blot: 5-hmC Antibody (C.15220001) [NBP2-59178] - 200 to 2 ng (equivalent of 10 to 0.1 pmol of C-base) of the hmC (1), mC (2) and C (3) PCR controls were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 4 ug/ml (dilution 1:500) of the 5-hmC Antibody (C.15220001), followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.](http://images.novusbio.com/fullsize/5-hmC-Antibody-C-15220001-Dot-Blot-NBP2-59178-img0005.jpg)