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![ELISA: Human Transportin 1 ELISA Kit (Colorimetric) [NBP2-76604] - Samples were spiked with high concentrations of Human Transportin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76604_human-transportin-1-elisa-kit-colorimetric-132202416225257.jpg "ELISA: Human Transportin 1 ELISA Kit (Colorimetric) [NBP2-76604] - Samples were spiked with high concentrations of Human Transportin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Transportin 1 ELISA Kit (Colorimetric) [NBP2-76604] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76604_human-transportin-1-elisa-kit-colorimetric-43202495039.jpg)
![Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/p53-[p-Thr81]-Antibody-Western-Blot-NBP3-16955-img0001.jpg "Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: ATR Antibody (4O3E0) [NBP3-33305] - Western blot analysis of various lysates using ATR Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33305_rabbit-atr-mab-4o3e0-91220241418152.jpg "Western Blot: ATR Antibody (4O3E0) [NBP3-33305] - Western blot analysis of various lysates using ATR Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: PP2A alpha [p Tyr307] Antibody (9B7Z9) [NBP3-16919] - Western blot analysis of extracts of NIH/3T3 cells, using PP2A alpha Rabbit mAb (NBP3-16919) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PP2A-alpha-p-Tyr307-Antibody-9B7Z9-Western-Blot-NBP3-16919-img0001.jpg "Western Blot: PP2A alpha [p Tyr307] Antibody (9B7Z9) [NBP3-16919] - Western blot analysis of extracts of NIH/3T3 cells, using PP2A alpha Rabbit mAb (NBP3-16919) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: p53 [p Ser392] Antibody [NB200-156] - rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k p53 phosphorylated Rat Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/p53-[p-Ser392]-Antibody-Western-Blot-NB200-156-img0001.jpg "Western Blot: p53 [p Ser392] Antibody [NB200-156] - rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k p53 phosphorylated Rat Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: PLK1 [p Thr210] Antibody [NB100-61108] - Rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NB100-61108-img0001.jpg "Western Blot: PLK1 [p Thr210] Antibody [NB100-61108] - Rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: Rad9 Antibody [NBP1-77978] - Used at a 1:200 dilution incubated 8 hr at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NBP1-77978-img0001.jpg "Western Blot: Rad9 Antibody [NBP1-77978] - Used at a 1:200 dilution incubated 8 hr at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.")
![Western Blot: Rad50 Antibody (S05-5H6) [NBP3-19512] - Western blot detection of Rad50 in K562, Hela cell lysates using NBP3-19512 (1:1000 diluted). Predicted band size: 154kDa. Observed band size: 154kDa.](http://images.novusbio.com/fullsize/Rad50-Antibody-S05-5H6-Western-Blot-NBP3-19512-img0001.jpg "Western Blot: Rad50 Antibody (S05-5H6) [NBP3-19512] - Western blot detection of Rad50 in K562, Hela cell lysates using NBP3-19512 (1:1000 diluted). Predicted band size: 154kDa. Observed band size: 154kDa.")
![Western Blot: Chk2 [p Ser19] Antibody [NBP3-13328] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Ser19) antibody (NBP3-13328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Chk2-[p-Ser19]-Antibody-Western-Blot-NBP3-13328-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk2 [p Ser19] Antibody [NBP3-13328] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Ser19) antibody (NBP3-13328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: HDAC1 Overexpression Lysate (Adult Normal) [NBL1-11477] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11477-img0002.jpg "Western Blot: HDAC1 Overexpression Lysate (Adult Normal) [NBL1-11477] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HDAC1.")
![Western Blot: Rad17 Overexpression Lysate (Adult Normal) [NBP2-09965] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rad17.](http://images.novusbio.com/fullsize/Rad17-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09965-img0001.jpg "Western Blot: Rad17 Overexpression Lysate (Adult Normal) [NBP2-09965] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rad17.")