| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97173-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95276-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06407-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98754-50ul | |||
![Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-288202412461654.jpg "Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32809) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-29820241492670.jpg)
![Western Blot: KIF2C Antibody (PSH01-51) [NBP3-32575] - Western blot analysis of KIF2C on different lysates with Rabbit anti-KIF2C antibody (NBP3-32575) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32575) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32575_rabbit-kif2c-mab-psh01-51-278202416285270.jpg "Western Blot: KIF2C Antibody (PSH01-51) [NBP3-32575] - Western blot analysis of KIF2C on different lysates with Rabbit anti-KIF2C antibody (NBP3-32575) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32575) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIF2C Antibody (PSH01-51) [NBP3-32575] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KIF2C antibody (NBP3-32575) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32575) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32575_rabbit-kif2c-mab-psh01-51-29820241433361.jpg)
![Western Blot: Cytokeratin 5/6 Antibody (PSH0-68) [NBP3-32252] - Western blot analysis of Cytokeratin 5/6 on different lysates with Rabbit anti-Cytokeratin 5/6 antibody (NBP3-32252) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: NCI-H226 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 60 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32252) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32252_rabbit-cytokeratin-5-6-mab-psh0-68-27820241631692.jpg "Western Blot: Cytokeratin 5/6 Antibody (PSH0-68) [NBP3-32252] - Western blot analysis of Cytokeratin 5/6 on different lysates with Rabbit anti-Cytokeratin 5/6 antibody (NBP3-32252) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: NCI-H226 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 60 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32252) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 5/6 Antibody (PSH0-68) [NBP3-32252] - Immunocytochemistry analysis of HeLa cells labeling Cytokeratin 5/6 with Rabbit anti-Cytokeratin 5/6 antibody (NBP3-32252) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 5/6 antibody (NBP3-32252) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32252_rabbit-cytokeratin-5-6-mab-psh0-68-29202410234288.jpg)
![Flow Cytometry: Cytokeratin 5/6 Antibody (PSH0-68) [NBP3-32252] - Flow cytometric analysis of A431 cells labeling Cytokeratin 5/6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32252, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32252_rabbit-cytokeratin-5-6-mab-psh0-68-29202416372146.jpg)
![Immunohistochemistry-Paraffin: FLRT1 Antibody [NBP2-97173] - Immunochemical staining of human FLRT1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FLRT1-Antibody-Immunohistochemistry-Paraffin-NBP2-97173-img0003.jpg "Immunohistochemistry-Paraffin: FLRT1 Antibody [NBP2-97173] - Immunochemical staining of human FLRT1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: FLRT1 Antibody [NBP2-97173] - Immunochemical staining of human FLRT1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FLRT1-Antibody-Immunohistochemistry-Paraffin-NBP2-97173-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FLRT1 Antibody [NBP2-97173] - Immunofluorescence staining of FLRT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97173 antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/nbp2-97173_rabbit-polyclonal-flrt1-antibody-235202511444487.png)
![Western Blot: Cytokeratin 5 Antibody [NBP2-95276] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:62 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-Western-Blot-NBP2-95276-img0003.jpg "Western Blot: Cytokeratin 5 Antibody [NBP2-95276] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:62 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody [NBP2-95276] - Immunofluorescence staining of Cytokeratin 5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-95276 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-95276_rabbit-polyclonal-cytokeratin-5-antibody-235202511444417.png)
![Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-288202412461622.jpg "Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (JE39-00) [NBP3-32833] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32833) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-298202414304140.jpg)
![Western Blot: KIFC1 Antibody [NBP3-06407] - KIFC1 Antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:74 kDa.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Western-Blot-NBP3-06407-img0001.jpg "Western Blot: KIFC1 Antibody [NBP3-06407] - KIFC1 Antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:74 kDa.")
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-06407] - Immunochemical staining of human KIFC1 in human tonsil with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-06407-img0003.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-06407] - Immunochemical staining of human KIFC1 in human lymph node with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-06407-img0002.jpg)
![Immunohistochemistry-Paraffin: Carm1 Antibody [NBP2-98754] - Immunochemical staining of human Carm1 in human ovarian cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NBP2-98754-img0003.jpg "Immunohistochemistry-Paraffin: Carm1 Antibody [NBP2-98754] - Immunochemical staining of human Carm1 in human ovarian cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Carm1 Antibody [NBP2-98754] - Immunochemical staining of human Carm1 in human prostatic carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NBP2-98754-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NBP2-98754] - Immunofluorescence staining of Carm1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98754 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98754_rabbit-polyclonal-carm1-antibody-23520251628120.png)
![Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-288202412442957.jpg "Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Immunocytochemistry analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes, then treated with lambda pp for 1 hour labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-29202410234248.jpg)
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-298202414333631.jpg "Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32127) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-29820241435244.jpg)
![Immunocytochemistry/ Immunofluorescence: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunocytochemistry analysis of 293T cells labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-2920241192312.jpg)
![Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-288202412461623.jpg "Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32808) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32808) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-298202414262664.jpg)
![Western Blot: PEA-15 Antibody [NBP2-88031] - WB Suggested Anti-PEA15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain](http://images.novusbio.com/fullsize/PEA-15-Antibody-Western-Blot-NBP2-88031-img0002.jpg "Western Blot: PEA-15 Antibody [NBP2-88031] - WB Suggested Anti-PEA15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain")
![Immunohistochemistry-Paraffin: PEA-15 Antibody [NBP2-88031] - Rabbit Anti-PEA15 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue. Observed Staining: Cytoplasmic. Primary Antibody Concentration: 1:100.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Immunohistochemistry-Paraffin-NBP2-88031-img0001.jpg)
![Western Blot: PEA-15 Antibody [NBP2-88031] - WB Suggested Anti-PEA15 Antibody. Positive Control: Lane 1: 20ug EGFP-transfected MCF7 lysate Lane 2: 20ug EGFP-PEA15 transfected MCF7 lysate. Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP. Secondry Antibody Dilution : 1:50](http://images.novusbio.com/fullsize/PEA-15-Antibody-Western-Blot-NBP2-88031-img0003.jpg)