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![Western Blot: GCR2 Antibody [NBP1-46214] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SGT1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SGT1 was also immunoprecipitated by rabbit anti-SGT1 antibody NBP1-46215, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/GCR2-Antibody-Western-Blot-NBP1-46214-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: GCR2 Antibody [NBP1-46214] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SGT1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SGT1 was also immunoprecipitated by rabbit anti-SGT1 antibody NBP1-46215, which recognizes a downstream epitope.")
![Immunoprecipitation: USP16 Antibody [NB100-81639] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody used for IP at 6 ug per reaction. USP16 was also immunoprecipitated by rabbit anti-USP16 antibody. For blotting immunoprecipitated USP16 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunoprecipitation-NB100-81639-img0001.jpg "Immunoprecipitation: USP16 Antibody [NB100-81639] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody used for IP at 6 ug per reaction. USP16 was also immunoprecipitated by rabbit anti-USP16 antibody. For blotting immunoprecipitated USP16 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: SPC24 Antibody [NBP2-32105] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SPC24 antibody NBP2-32105 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SPC24-Antibody-Western-Blot-NBP2-32105-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: SPC24 Antibody [NBP2-32105] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SPC24 antibody NBP2-32105 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody (3C11) [NBP3-15197] - Immunofluorescence staining of HepG2 Cells with Skp2 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Skp2-Antibody-3C11-Immunocytochemistry-Immunofluorescence-NBP3-15197-img0002.jpg "Immunocytochemistry/Immunofluorescence: Skp2 Antibody (3C11) [NBP3-15197] - Immunofluorescence staining of HepG2 Cells with Skp2 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Skp2 Antibody (3C11) [NBP3-15197] - Overlay histogram showing Hela cells stained with Skp2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/Skp2-Antibody-3C11-Flow-Cytometry-NBP3-15197-img0001.jpg)
![ELISA: DLL3 Antibody (7B7) [NBP3-26618] - The Binding Activity of DLL3 with DLL3 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized DLL3 at 2 ug/mL can bind DLL3 Antibody, the EC50 is 1.625-2.702 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26618_human-dll3-mab-7b7-262202412272346.jpg "ELISA: DLL3 Antibody (7B7) [NBP3-26618] - The Binding Activity of DLL3 with DLL3 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized DLL3 at 2 ug/mL can bind DLL3 Antibody, the EC50 is 1.625-2.702 ng/mL.")
![ELISA: DLL3 Antibody (7B7) [NBP3-26618] - The Binding Activity of DLL3 with DLL3 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized DLL3 at 2 ug/mL can bind DLL3 Antibody, the EC50 is 1.102-1.707 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26618_human-dll3-mab-7b7-262202412272316.jpg)
![Flow Cytometry: DLL3 Antibody (7B7) [NBP3-26618] - Untransfected HEK293 cells (green line) and transfected Human DLL3 HEK293 stable cells (red line) were stained with anti-DLL3 antibody (rovalpituzumab-like) (2ul/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG1 Fc antibody and analyzed with flow cytometry.](http://images.novusbio.com/fullsize/nbp3-26618_human-dll3-mab-7b7-282202414312733.jpg)