| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93990-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93798-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35835-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37968-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52519-0.025mg | |||
![Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-7W3E3-Western-Blot-NBP3-15821-img0004.jpg "Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded rat brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512435347.jpg)
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded mouse brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512432070.jpg)
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded human colon carcinoma tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512443371.jpg)
![Western Blot: EB3 Antibody (7J9V3) [NBP3-15904] - Western blot analysis of extracts of various cell lines, using EB3 Rabbit mAb (NBP3-15904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/EB3-Antibody-7J9V3-Western-Blot-NBP3-15904-img0004.jpg "Western Blot: EB3 Antibody (7J9V3) [NBP3-15904] - Western blot analysis of extracts of various cell lines, using EB3 Rabbit mAb (NBP3-15904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of U-2 OS cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112146..jpg)
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C6 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112350..jpg)
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C2C12 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112518..jpg)
![Western Blot: Ninein Antibody [NBP2-93990] - Analysis of extracts of Jurkat cells, using Ninein .Exposure time: 90s.](http://images.novusbio.com/fullsize/Ninein-Antibody-Western-Blot-NBP2-93990-img0005.jpg "Western Blot: Ninein Antibody [NBP2-93990] - Analysis of extracts of Jurkat cells, using Ninein .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded rat kidney using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0004.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded human stomach using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0002.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded mouse lung using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0003.jpg)
![Western Blot: DIS3 Antibody [NBP2-81804] - Analysis of DIS3 in human ovary tissue lysate with DIS3 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/DIS3-Antibody-Western-Blot-NBP2-81804-img0001.jpg "Western Blot: DIS3 Antibody [NBP2-81804] - Analysis of DIS3 in human ovary tissue lysate with DIS3 antibody at 1 ug/ml.")
![Immunohistochemistry: DIS3 Antibody - BSA Free [NBP2-81804] - Immunohistochemistry of DIS3 in mouse testis tissue with DIS3 antibody at 2.5 u/ml.](http://images.novusbio.com/fullsize/nbp2-81804_rabbit-polyclonal-dis3-antibody-2711202420352429.jpg)
![Immunocytochemistry/ Immunofluorescence: DIS3 Antibody - BSA Free [NBP2-81804] - Immunofluorescence of DIS3 in mouse testis tissue with DIS3 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81804_rabbit-polyclonal-dis3-antibody-2711202420263219.jpg)
![Western Blot: PPP2R2A Antibody [NBP2-93798] - Analysis of extracts of various cell lines, using PPP2R2A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Western-Blot-NBP2-93798-img0004.jpg "Western Blot: PPP2R2A Antibody [NBP2-93798] - Analysis of extracts of various cell lines, using PPP2R2A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: PPP2R2A Antibody [NBP2-93798] - Rat kidney using PPP2R2A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Immunohistochemistry-Paraffin-NBP2-93798-img0003.jpg)
![Immunohistochemistry-Paraffin: PPP2R2A Antibody [NBP2-93798] - Human esophagus using PPP2R2A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Immunohistochemistry-Paraffin-NBP2-93798-img0001.jpg)
![Immunohistochemistry-Paraffin: PPP2R2A Antibody [NBP2-93798] - Mouse liver using /PPP2R2A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Immunohistochemistry-Paraffin-NBP2-93798-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: CNAP1 Antibody [NBP3-35835] - Immunofluorescence analysis of C6 cells using CNAP1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35835_rabbit-cnap1-pab-912202415143623.jpg "Immunocytochemistry/ Immunofluorescence: CNAP1 Antibody [NBP3-35835] - Immunofluorescence analysis of C6 cells using CNAP1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: CNAP1 Antibody [NBP3-35835] - Western blot analysis of various lysates using CNAP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35835_rabbit-cnap1-pab-912202414123867.jpg)
![Immunocytochemistry/ Immunofluorescence: CNAP1 Antibody [NBP3-35835] - Immunofluorescence analysis of U2OS cells using CNAP1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35835_rabbit-cnap1-pab-9122024159562.jpg)
![Immunocytochemistry/ Immunofluorescence: CNAP1 Antibody [NBP3-35835] - Immunofluorescence analysis of L929 cells using CNAP1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35835_rabbit-cnap1-pab-912202415161852.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-1012202411545578.jpg "Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: Cullin 1 Antibody [NBP3-37968] - Western blot analysis of various lysates, using Cullin 1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-101220241243916.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-1012202411572689.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-101220241252829.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mouse mAb against Hela (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0005.jpg "Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mouse mAb against Hela (1), and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis of paraffin-embedded ovarian cancer tissues using NME2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Immunohistochemistry-Paraffin-NBP2-52519-img0004.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - nalysis using NME2 mAb against HEK293 (1) and NME2 (AA: FULL(1-152))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0003.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mAb against human NME2 (AA: FULL(1-152)) recombinant protein. (Expected MW is 43.2 kDa)](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0002.jpg)
![ELISA: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-ELISA-NBP2-52519-img0007.jpg)
![Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-278202415295570.jpg "Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-278202415242669.jpg "Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32579) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-298202413585583.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunocytochemistry analysis of SiHa cells labeling MCM6 with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-29202410234240.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)
![Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-27820241633125.png "Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32288) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-29820241435277.jpg)
![Immunocytochemistry/ Immunofluorescence: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunocytochemistry analysis of 293 cells labeling PA2G4/EBP1 with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-2920241192382.jpg)