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![Western Blot: AMSH/STAMBP Antibody (5S2I1) [NBP3-33217] - Western blot analysis of various lysates using AMSH/STAMBP Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33217_rabbit-amsh-stambp-mab-5s2i1-1012202412105.jpg "Western Blot: AMSH/STAMBP Antibody (5S2I1) [NBP3-33217] - Western blot analysis of various lysates using AMSH/STAMBP Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: Abeta 40 Antibody (PSH02-82) [NBP3-32077] - Immunohistochemical analysis of paraffin-embedded mouse model of Alzheimer's brain tissue with Rabbit anti-Abeta 40 antibody (NBP3-32077) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32077) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32077_rabbit-abeta-40-mab-psh02-82-29820241435226.jpg "Immunohistochemistry: Abeta 40 Antibody (PSH02-82) [NBP3-32077] - Immunohistochemical analysis of paraffin-embedded mouse model of Alzheimer's brain tissue with Rabbit anti-Abeta 40 antibody (NBP3-32077) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32077) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP2-44217] - Lane 1: MDA-MB-231 cells. Lane 2: MDA-MB-231 cells treated with TGF-beta1 for 1h. Load: 20 Ug per lane.](http://images.novusbio.com/fullsize/Smad2-3-[p-Ser-465]-Antibody-Western-Blot-NBP2-44217-img0001.jpg "Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP2-44217] - Lane 1: MDA-MB-231 cells. Lane 2: MDA-MB-231 cells treated with TGF-beta1 for 1h. Load: 20 Ug per lane.")
![Wild-type (WT) and ZAK knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAK antibody [N3C2], Internal (NBP2-20934) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20934_rabbit-polyclonal-zak-antibody-western-blot-2611202518235323.jpg "<b>Genetic Strategies Validation. </b>Wild-type (WT) and ZAK knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAK antibody [N3C2], Internal (NBP2-20934) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: BIN2 Antibody [NBP2-15588] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BIN2-Antibody-Western-Blot-NBP2-15588-img0001.jpg "Western Blot: BIN2 Antibody [NBP2-15588] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Septin-11 Antibody [NBP2-20299] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP2-20299-img0001.jpg "Western Blot: Septin-11 Antibody [NBP2-20299] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: OSTM1 Antibody [NBP2-19641] - Sample (30 ug of whole cell lysate) A: Raji B: HL-60 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/OSTM1-Antibody-Western-Blot-NBP2-19641-img0001.jpg "Western Blot: OSTM1 Antibody [NBP2-19641] - Sample (30 ug of whole cell lysate) A: Raji B: HL-60 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Flotillin-1 Antibody (S08-0G5) [NBP3-14932] - Western blot detection of Flotillin-1 in Hela, A549, MCF-7, U251 cell lysates using Flotillin-1 Rabbit mAb (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 49kDa.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-S08-0G5-Western-Blot-NBP3-14932-img0001.jpg "Western Blot: Flotillin-1 Antibody (S08-0G5) [NBP3-14932] - Western blot detection of Flotillin-1 in Hela, A549, MCF-7, U251 cell lysates using Flotillin-1 Rabbit mAb (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 49kDa.")
![Western Blot: EGFR [p Thr669] Antibody [NBP3-13264] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13264) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Thr669]-Antibody-Western-Blot-NBP3-13264-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Thr669] Antibody [NBP3-13264] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13264) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR [p Thr669] Antibody [NBP3-13226] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13226) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Thr669]-Antibody-Western-Blot-NBP3-13226-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Thr669] Antibody [NBP3-13226] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13226) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR [p Ser1047, p Ser1046] Antibody [NBP3-13230] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Ser1047--p-Ser1046]-Antibody-Western-Blot-NBP3-13230-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Ser1047, p Ser1046] Antibody [NBP3-13230] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR [p Ser1046, p Ser1047] Antibody [NBP3-13262] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Ser1046--p-Ser1047]-Antibody-Western-Blot-NBP3-13262-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Ser1046, p Ser1047] Antibody [NBP3-13262] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")