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![Western Blot: Smad2 [p Ser255, p Ser245, p Ser250] Antibody [NBP3-13246] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser245/Ser250/Ser255) antibody (NBP3-13246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad2-[p-Ser255--p-Ser245--p-Ser250]-Antibody-Western-Blot-NBP3-13246-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Smad2 [p Ser255, p Ser245, p Ser250] Antibody [NBP3-13246] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser245/Ser250/Ser255) antibody (NBP3-13246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1A antibody [HL2415] (NBP3-48683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48683_rabbit-rap1a-mab-hl2415-western-blot-141120251843317.jpg "Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1A antibody [HL2415] (NBP3-48683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: GEF-H1 Antibody [NBP3-13111] - Each well was loaded one brain homogenate as indicated were separated by 4-20% SDS-PAGE, and the membrane was blotted with CG10188 antibody (NBP3-13111) diluted at 1:5000.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-Western-Blot-NBP3-13111-img0001.jpg "Western Blot: GEF-H1 Antibody [NBP3-13111] - Each well was loaded one brain homogenate as indicated were separated by 4-20% SDS-PAGE, and the membrane was blotted with CG10188 antibody (NBP3-13111) diluted at 1:5000.")
![Western Blot: CDC25C [p Ser216] Antibody [NBP3-12879] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C (phospho Ser216) antibody (NBP3-12879) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDC25C-[p-Ser216]-Antibody-Western-Blot-NBP3-12879-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDC25C [p Ser216] Antibody [NBP3-12879] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C (phospho Ser216) antibody (NBP3-12879) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR [p Ser1046, p Ser1047] Antibody [NBP3-13262] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Ser1046--p-Ser1047]-Antibody-Western-Blot-NBP3-13262-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Ser1046, p Ser1047] Antibody [NBP3-13262] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: SMC2 Antibody (GT4312) [NBP3-13601] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC2 antibody [GT4312] (NBP3-13601) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SMC2-Antibody-GT4312-Western-Blot-NBP3-13601-img0001.jpg "Western Blot: SMC2 Antibody (GT4312) [NBP3-13601] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC2 antibody [GT4312] (NBP3-13601) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: EGFR [p Thr669] Antibody [NBP3-13226] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13226) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Thr669]-Antibody-Western-Blot-NBP3-13226-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Thr669] Antibody [NBP3-13226] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13226) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR [p Ser1047, p Ser1046] Antibody [NBP3-13230] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Ser1047--p-Ser1046]-Antibody-Western-Blot-NBP3-13230-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Ser1047, p Ser1046] Antibody [NBP3-13230] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR [p Thr669] Antibody (GT1361) [NBP3-13645] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody [GT1361] (NBP3-13645) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-p-Thr669-Antibody-GT1361-Western-Blot-NBP3-13645-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Thr669] Antibody (GT1361) [NBP3-13645] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody [GT1361] (NBP3-13645) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
