| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35288-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61731-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06477-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99631-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52685-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75903SS | |||
![Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-10122024120667.jpg "Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk7 Antibody [NBP3-35288] - Western Blot analysis of lysates from wild type (WT) and Cdk7 knockout (KO) 293T cells, using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-31120251744483.jpg%3Ft=1709093634)
![Western Blot: CDC37 Antibody (6B3B5) [NBP2-61731] - Analysis using CDC37 mAb against HEK293 (1) and CDC37 (AA: 241-378)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CDC37-Antibody-6B3B5-Western-Blot-NBP2-61731-img0003.jpg "Western Blot: CDC37 Antibody (6B3B5) [NBP2-61731] - Analysis using CDC37 mAb against HEK293 (1) and CDC37 (AA: 241-378)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: CDC37 Antibody (6B3B5) [NBP2-61731] - Analysis using CDC37 mAb against human CDC37 (AA: 241-378) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/CDC37-Antibody-6B3B5-Western-Blot-NBP2-61731-img0002.jpg)
![Western Blot: CDC37 Antibody (6B3B5) [NBP2-61731] - Analysis using CDC37 mouse mAb against K562 (1), LNcap (2), A431 (3), and HEK293 (4) cell lysate.](http://images.novusbio.com/fullsize/CDC37-Antibody-6B3B5-Western-Blot-NBP2-61731-img0004.jpg)
![ELISA: CDC37 Antibody (6B3B5) [NBP2-61731] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CDC37-Antibody-6B3B5-ELISA-NBP2-61731-img0001.jpg)
![Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-288202412461681.jpg "Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Immunocytochemistry analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202410484836.jpg)
![Flow Cytometry: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Flow cytometric analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32760, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202416453235.jpg)
![Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-278202415295577.jpg "Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HRAS Antibody (JE42-15) [NBP3-32413] - Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-298202414161896.jpg)
![Western Blot: MNAT1 Antibody [NBP3-06477] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa](http://images.novusbio.com/fullsize/MNAT1-Antibody-Western-Blot-NBP3-06477-img0001.jpg "Western Blot: MNAT1 Antibody [NBP3-06477] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa")
![Immunoprecipitation: MNAT1 Antibody [NBP3-06477] - Lane A:0.5 mg HELA Whole Cell Lysate4 uL anti-MNAT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MNAT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :35 kDa](http://images.novusbio.com/fullsize/MNAT1-Antibody-Immunoprecipitation-NBP3-06477-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MNAT1 Antibody [NBP3-06477] - Staining of MNAT1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06477 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06477_rabbit-polyclonal-mnat1-antibody-235202511444422.png)
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 33 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-99631-img0005.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 33 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - 14-3-3 sigma/Stratifin was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 2 ul anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 32 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunoprecipitation-NBP2-99631-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Immunofluorescence staining of 14-3-3 sigma/Stratifin in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99631 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99631_rabbit-polyclonal-14-3-3-sigma-stratifin-antibody-23520251433152.png)
![Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-278202415212411.jpg "Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Western-Blot-NBP1-44894-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0002.jpg)
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0003.jpg)
![Flow Cytometry: EGFR Antibody (Matuzumab) - Chimeric [NBP2-52685] - Flow-cytometry using the anti-EGFR antibody Matuzumab. HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (NBP2-52685, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/EGFR-Antibody-Matuzumab-Chimeric-Flow-Cytometry-NBP2-52685-img0002.jpg "Flow Cytometry: EGFR Antibody (Matuzumab) - Chimeric [NBP2-52685] - Flow-cytometry using the anti-EGFR antibody Matuzumab. HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (NBP2-52685, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (Matuzumab) - Chimeric - Azide and BSA Free [NBP2-52685] - Immunofluorescence staining of fixed A431 cells with anti-EGFR antibody Matuzumab. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP2-52685 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52685, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52685_rabbit-monoclonal-egfr-antibody-matuzumab-chimeric-74202522141213.png)
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M548) [NBP3-23234] - Immunocytochemical labeling of p38 alpha in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38 alpha (NBP3-23234). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23234_mouse-p38-alpha-mab-m548-199202318202923.jpg "Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M548) [NBP3-23234] - Immunocytochemical labeling of p38 alpha in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38 alpha (NBP3-23234). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Western Blot: p38 alpha Antibody (M548) [NBP3-23234] - Western blot analysis of human A431 cells (lanes 1 & 2) and rat A7r5 cells (lanes 3 & 4). The blot was probed with mouse monoclonal anti-p38 alpha (C-terminal) clone M548 at a dilution of 1:250 (lanes 1 & 3) or 1:1000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23234_mouse-p38-alpha-mab-m548-19920231856916.jpg)
![Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-Immunohistochemistry-NBP2-75903-img0002.jpg "Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.")
![ELISA: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - ELISA Plate coated with human EGFR-Fc at a concentration of 2.0 mg/ml. A 2-fold serial dilution from 100 to 0.1 ng/ml was performed using HRP labelled cetuximab human IgG1 (NBP2-75903).](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-ELISA-NBP2-75903-img0001.jpg)