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![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-45125 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45125, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-45125_rabbit-p16ink4a-cdkn2a-mab-30859-s17-chimeric-bsa-free-immunocytochemistry-immunofluorescence-222026035554.png "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-45125 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45125, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.")
![Immunoprecipitation: MAX binding protein Antibody [NBP2-04053] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MNT antibody NBP2-04052 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MNT was also immunoprecipitated by rabbit anti-MNT antibody NBP2-04053, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/MAX-binding-protein-Antibody-Immunoprecipitation-NBP2-04053-img0001.jpg "Immunoprecipitation: MAX binding protein Antibody [NBP2-04053] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MNT antibody NBP2-04052 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MNT was also immunoprecipitated by rabbit anti-MNT antibody NBP2-04053, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![Western Blot: RFC1 Antibody [NB600-961] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RFC1 antibody NB600-961 (lot 2 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. RFC1 was also immunoprecipitated by prior lot 1 of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB600-961-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: RFC1 Antibody [NB600-961] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RFC1 antibody NB600-961 (lot 2 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. RFC1 was also immunoprecipitated by prior lot 1 of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")