| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54687-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87327-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08439 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08455 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP3-18536 | |||
![Western Blot: alpha Tubulin Antibody [NBP2-54687] - Analysis NBP2-54687 (A) shows similar pattern to independent antibody NBP2-54708 (B). Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Western-Blot-NBP2-54687-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: alpha Tubulin Antibody [NBP2-54687] - Analysis NBP2-54687 (A) shows similar pattern to independent antibody NBP2-54708 (B). Alpha tubulin molecular weight: 50 kDa.")
![<b>Independent Antibodies Validation. </b>Western Blot: alpha Tubulin Antibody [NBP2-54687] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Western-Blot-NBP2-54687-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody [NBP2-54687] - Staining of human cell line U-251 MG shows localization to microtubules. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54687-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54687] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunohistochemistry-Paraffin-NBP2-54687-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54687] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunohistochemistry-Paraffin-NBP2-54687-img0008.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54687] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunohistochemistry-Paraffin-NBP2-54687-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54687] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunohistochemistry-Paraffin-NBP2-54687-img0010.jpg)
![Western Blot: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody at 1/500 dilution. Lane 1: PC-12 cell lysate, Lane 2: Hela cell lysateLane 3: NIH/3T3 cell lysate, Lane 4: SH-SY5Y cell lysate. Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Western-Blot-NBP2-67374-img0006.jpg "Western Blot: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody at 1/500 dilution. Lane 1: PC-12 cell lysate, Lane 2: Hela cell lysateLane 3: NIH/3T3 cell lysate, Lane 4: SH-SY5Y cell lysate. Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Staining Tubulin beta-III in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Immunocytochemistry-Immunofluorescence-NBP2-67374-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Analysis of paraffin-embedded mouse brain tissue using anti-Tubulin beta-III antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Immunohistochemistry-Paraffin-NBP2-67374-img0003.jpg)
![Flow Cytometry: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Flow cytometric analysis of beta-III Tubulin was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Flow-Cytometry-NBP2-67374-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Staining Tubulin beta-III in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Immunocytochemistry-Immunofluorescence-NBP2-67374-img0001.jpg)
![Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP2-24679-img0002.jpg "Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.")
![Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP2-24679] - Immunohistochemical analysis of IFRD1 in formalin-fixed, paraffin-embedded humanrectum tissue using an isotype control (top) and NBP2-24679 (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Immunohistochemistry-Paraffin-NBP2-24679-img0001.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-288202414233110.jpg "Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241435230.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33140, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241492693.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29202410484841.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33140, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-392024884679.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta-III Tubulin protein stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Immunocytochemistry-Immunofluorescence-NBP3-13505-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (NBP3-13505) diluted by 1:1000.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0001.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0003.jpg)
![beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13505_mouse-beta-iii-tubulin-mab-gt1338-immunohistochemistry-paraffin-2611202518393116.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-28820241423313.jpg "Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29820241435223.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29202410484898.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-39202488464.jpg)
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)
![Western Blot: IFRD1 Antibody [NBP1-87327] - Analysis in control (vector only transfected HEK293T lysate) and IFRD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP1-87327-img0006.jpg "Western Blot: IFRD1 Antibody [NBP1-87327] - Analysis in control (vector only transfected HEK293T lysate) and IFRD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: IFRD1 Antibody [NBP1-87327] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87327-img0005.jpg)
![Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP1-87327] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-87327-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (S02-4G7) [NBP3-19582] - Western blot detection of beta-III Tubulin in Rat Brain, Hela cell lysates using NBP3-19582 (1:1000 diluted). Predicted band size: 50kDa. Observed band size: 50kDa.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-S02-4G7-Western-Blot-NBP3-19582-img0003.jpg "Western Blot: beta-III Tubulin Antibody (S02-4G7) [NBP3-19582] - Western blot detection of beta-III Tubulin in Rat Brain, Hela cell lysates using NBP3-19582 (1:1000 diluted). Predicted band size: 50kDa. Observed band size: 50kDa.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (S02-4G7) [NBP3-19582] - Immunofluorescence of beta-III Tubulin (green) in Hela using NBP3-19582 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-S02-4G7-Immunocytochemistry-Immunofluorescence-NBP3-19582-img0001.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (S02-4G7) [NBP3-19582] - Immunohistochemistry of beta-III Tubulin in paraffin-embedded Human Cholangiocarcinoma using NBP3-19582 at dilution 1/50](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-S02-4G7-Immunohistochemistry-Paraffin-NBP3-19582-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (TUBB3/3731) [NBP3-07385] - Western Blot Analysis of U87 cell lysate using beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3731).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-TUBB3-3731-Western-Blot-NBP3-07385-img0003.jpg "Western Blot: beta-III Tubulin Antibody (TUBB3/3731) [NBP3-07385] - Western Blot Analysis of U87 cell lysate using beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3731).")
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (TUBB3/3731) [NBP3-07385] - Formalin-fixed, paraffin-embedded human Brain stained with beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3731).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-TUBB3-3731-Immunohistochemistry-Paraffin-NBP3-07385-img0002.jpg)
![Protein Array: beta-III Tubulin Antibody (TUBB3/3731) [NBP3-07385] - Analysis of Protein Array containing more than 21,000 full-length human proteins using beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3731).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-TUBB3-3731-Protein-Array-NBP3-07385-img0001.jpg)
![Western Blot: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386] - Western Blot Analysis of U87 cell lysate using beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3732).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-TUBB3-3732-Western-Blot-NBP3-07386-img0003.jpg "Western Blot: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386] - Western Blot Analysis of U87 cell lysate using beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3732).")
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3732).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-TUBB3-3732-Immunohistochemistry-Paraffin-NBP3-07386-img0002.jpg)
![Protein Array: beta-III Tubulin Antibody (TUBB3/3732) [NBP3-07386] - Analysis of Protein Array containing more than 21,000 full-length human proteins using beta-III Tubulin Mouse Monoclonal Antibody (TUBB3/3732).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-TUBB3-3732-Protein-Array-NBP3-07386-img0001.jpg)
![Western Blot: alpha Tubulin Antibody (RM113) [Biotin] [NBP2-77401] - Western blot of A431 cells, using NBP3-18536 at 1/1000 dilution, showed a band of alpha Tubulin at the predicted MW (~50 kDa).](http://images.novusbio.com/fullsize/nbp2-77401_rabbit-monoclonal-alpha-tubulin-antibody-rm113-western-blot-18920252347485.jpg "Western Blot: alpha Tubulin Antibody (RM113) [Biotin] [NBP2-77401] - Western blot of A431 cells, using NBP3-18536 at 1/1000 dilution, showed a band of alpha Tubulin at the predicted MW (~50 kDa).")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (RM113) [NBP2-77401] - Staining of HeLa cells, using NBP2-77401 (red). Nuclei have been labeled with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-77401_rabbit-monoclonal-alpha-tubulin-antibody-rm113-immunocytochemistry-immunofluorescence-199202502436.png)