| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03535-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05393-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52513-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98122-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98309-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB5729-SP | |||
![Immunocytochemistry/ Immunofluorescence: PIDD Antibody - BSA Free [PIDD] - Immunofluorescence analysis of HeLa cells using PIDD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03535_rabbit-polyclonal-lrdd-antibody-52202516334114.jpg "Immunocytochemistry/ Immunofluorescence: PIDD Antibody - BSA Free [PIDD] - Immunofluorescence analysis of HeLa cells using PIDD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded mouse brain using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0004.jpg)
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded human lung cancer using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0002.jpg)
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded rat brain using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0003.jpg)
![Western Blot: FOXO4 [p Ser197] Antibody [NBP3-05393] - Western blot analysis of extracts from 293 cells using phospho-FOXO4-S197 antibody (NBP3-05393). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/FOXO4-[p-Ser197]-Antibody-Western-Blot-NBP3-05393-img0003.jpg "Western Blot: FOXO4 [p Ser197] Antibody [NBP3-05393] - Western blot analysis of extracts from 293 cells using phospho-FOXO4-S197 antibody (NBP3-05393). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/Immunofluorescence: FOXO4 [p Ser197] Antibody [NBP3-05393] - Immunofluorescence analysis of methanol-fixed MCF-7 cells using FOXO4 antibody (NBP3-05393).](http://images.novusbio.com/fullsize/FOXO4-[p-Ser197]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05393-img0002.jpg)
![Immunohistochemistry-Paraffin: FOXO4 [p Ser197] Antibody [NBP3-05393] - Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using FOXO4 antibody (NBP3-05393).](http://images.novusbio.com/fullsize/FOXO4-[p-Ser197]-Antibody-Immunohistochemistry-Paraffin-NBP3-05393-img0001.jpg)
![Western Blot: FOXO4 [p Ser197] Antibody - Azide and BSA Free [FOXO4] - Western blot analysis of various lysates, using FOXO4 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-05393_rabbit-polyclonal-foxo4-p-ser197-antibody-5220251638055.jpg)
![Western Blot: FOXO4 [p Ser197] Antibody - Azide and BSA Free [FOXO4] - Western blot analysis of various lysates, using FOXO4 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-05393_rabbit-polyclonal-foxo4-p-ser197-antibody-5220251639662.jpg)
![Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Western-Blot-NBP3-15627-img0009.jpg "Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts from normal (control) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Knockout-Validated-NBP3-15627-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with a-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100 x.](http://images.novusbio.com/fullsize/nbp3-15627_rabbit-cyclin-d1-mab-7c8z4-immunocytochemistry-immunofluorescence-2092023105125.jpg)
![Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against human mTOR (AA: 2311-2529) recombinant protein. (Expected MW is 50.8 kDa)](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Western-Blot-NBP2-52513-img0003.jpg "Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against human mTOR (AA: 2311-2529) recombinant protein. (Expected MW is 50.8 kDa)")
![Immunohistochemistry-Paraffin: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis of endometrial cancer tissues using mTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Immunohistochemistry-NBP2-52513-img0002.jpg)
![Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against HEK293 (1) and mTOR (AA: 2311-2529)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Western-Blot-NBP2-52513-img0004.jpg)
![Immunohistochemistry-Paraffin: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis of brain tissues using mTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Immunohistochemistry-NBP2-52513-img0001.jpg)
![ELISA: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-ELISA-NBP2-52513-img0005.jpg)
![Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).](http://images.novusbio.com/fullsize/CDK4-Antibody-DCS-31-Western-Blot-NB120-6315-img0001.jpg "Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).")
![Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-98122-img0002.jpg "Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse intestine with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0004.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0003.jpg)
![Immunoprecipitation: CDC37 Antibody [NBP2-98122] - Mouse CDC37 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-Mouse CDC37 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse CDC37 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-98122-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of CDC37 in mouse kidney with NBP2-98122 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp2-98122_rabbit-polyclonal-cdc37-antibody-23520252135161.png)
![Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-27820241631628.jpg "Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32173) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29820241420958.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunocytochemistry analysis of HeLa cells labeling CDC25C with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-2920241023428.jpg)
![Flow Cytometry: CDC25C Antibody (JE59-53) [NBP3-32173] - Flow cytometric analysis of HeLa cells labeling CDC25C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32173, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29202416453230.jpg)
![Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29820241420932.jpg "Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: EGFR Antibody (PD00-75) [NBP3-32255] - Immunocytochemistry analysis of A431 cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32255) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-2920241192377.jpg)
![Flow Cytometry: EGFR Antibody (PD00-75) [NBP3-32255] - Flow cytometric analysis of A431 cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32255, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29202416372141.jpg)
![Western Blot: EGFR Antibody (PD00-75) [NBP3-32255] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32255) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MDA-MB-468 cell lysate Lane 4: MCF7 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 134 kDa Observed band size: 150 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-3920248485012.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-278202416331255.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29820241426265.jpg)
![Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunocytochemistry analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A with Rabbit anti-p21/CIP1/CDKN1A/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202410334083.jpg)
![Flow Cytometry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Flow cytometric analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32691, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202416372178.jpg)
![Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Western-Blot-NBP2-67686-img0004.jpg "Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds")
![Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0002.jpg)
![Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: NIH/3T3 cell lysate (10 ug/Lane)Lane 3: Mouse spleen tissue lysate (20 ug/Lane)Predicted band size: 34 kDa Observed band size: 35 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Western-Blot-NBP2-67686-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0001.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-98309-img0006.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunochemical staining of mouse 14-3-3 sigma/Stratifin in mouse skin with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP2-98309-img0004.jpg)
![Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Mouse 14-3-3 sigma/Stratifin was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 ul anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunoprecipitation-NBP2-98309-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunofluorescence staining of 14-3-3 sigma/Stratifin in Raw264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98309 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98309_rabbit-polyclonal-14-3-3-sigma-stratifin-antibody-23520252135137.png)
![Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Immunocytochemistry-MAB5729-img0002.jpg "Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.")
![Flow Cytometry: WT1 Antibody (960522) [MAB5729] - An intracellular stain was performed on THP-1 cells with WT1 [960522] Antibody MAB5729AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Flow-Cytometry-MAB5729-img0003.jpg)