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![Immunoprecipitation:WIPI2 AntibodyNBP3-29825] -Detection of human WIPI2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WIPI2 antibody NBP3-29825 (lot NBP3-29825-1) used for IP at 6 µg per reaction. WIPI2 was also immunoprecipitated by rabbit anti-WIPI2 antibody. For blotting immunoprecipitated WIPI2, NBP3-29825 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29825_rabbit-wipi2-pab-301220241924716.jpg "Immunoprecipitation:WIPI2 AntibodyNBP3-29825] -Detection of human WIPI2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WIPI2 antibody NBP3-29825 (lot NBP3-29825-1) used for IP at 6 µg per reaction. WIPI2 was also immunoprecipitated by rabbit anti-WIPI2 antibody. For blotting immunoprecipitated WIPI2, NBP3-29825 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:CCDC47 AntibodyNBP3-30245] -Detection of human CCDC47 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCDC47 antibody NBP3-30245 (lot NBP3-30245-1) used for IP at 6 µg per reaction. CCDC47 was also immunoprecipitated by rabbit anti-CCDC47.. For blotting immunoprecipitated CCDC47, NBP3-30245 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30245_rabbit-ccdc47-pab-3012202419385012.jpg "Immunoprecipitation:CCDC47 AntibodyNBP3-30245] -Detection of human CCDC47 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCDC47 antibody NBP3-30245 (lot NBP3-30245-1) used for IP at 6 µg per reaction. CCDC47 was also immunoprecipitated by rabbit anti-CCDC47.. For blotting immunoprecipitated CCDC47, NBP3-30245 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:[NBP3-30712]-Detection of human PAN2/USP52 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PAN2/USP52 antibody used for IP at 6 µg per reaction. PAN2/USP52 was also immunoprecipitated by rabbit anti-PAN2/USP52 antibody. For blotting immunoprecipitated PAN2/USP52, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30712_rabbit-usp52-pab-231202512101690.jpg "Immunoprecipitation:[NBP3-30712]-Detection of human PAN2/USP52 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PAN2/USP52 antibody used for IP at 6 µg per reaction. PAN2/USP52 was also immunoprecipitated by rabbit anti-PAN2/USP52 antibody. For blotting immunoprecipitated PAN2/USP52, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot:[NBP3-29084]-Detection of mouse SIL by western blot. Samples: Whole cell lysate from TCMK-1, 4T1, and CT26. WT cells. Antibodies: Affinity purified rabbit anti-SIL antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29084_rabbit-stil-pab-231202511585576.jpg "Western Blot:[NBP3-29084]-Detection of mouse SIL by western blot. Samples: Whole cell lysate from TCMK-1, 4T1, and CT26. WT cells. Antibodies: Affinity purified rabbit anti-SIL antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:UBE2S AntibodyNBP3-29545] -Detection of human E2-EPF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-E2-EPF antibody NBP3-29545 (lot NBP3-29545-1) used for IP at 6 µg/mg lysate. E2-EPF was also immunoprecipitated by rabbit anti-E2-EPF.. For blotting immunoprecipitated E2-EPF, NBP3-29545 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29545_rabbit-ube2s-pab-301220241551161.jpg "Immunoprecipitation:UBE2S AntibodyNBP3-29545] -Detection of human E2-EPF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-E2-EPF antibody NBP3-29545 (lot NBP3-29545-1) used for IP at 6 µg/mg lysate. E2-EPF was also immunoprecipitated by rabbit anti-E2-EPF.. For blotting immunoprecipitated E2-EPF, NBP3-29545 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:FLJ10808 AntibodyNBP3-29188] -Detection of human UBA6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-UBA6 antibody NBP3-29188 (lot NBP3-29188-1) used for IP at 6 µg/mg lysate. UBA6 was also immunoprecipitated by rabbit anti-UBA6 antibodies. For blotting immunoprecipitated UBA6, NBP3-29188 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29188_rabbit-flj10808-pab-3012202412293924.jpg "Immunoprecipitation:FLJ10808 AntibodyNBP3-29188] -Detection of human UBA6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-UBA6 antibody NBP3-29188 (lot NBP3-29188-1) used for IP at 6 µg/mg lysate. UBA6 was also immunoprecipitated by rabbit anti-UBA6 antibodies. For blotting immunoprecipitated UBA6, NBP3-29188 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot:[NBP3-30013]-Detection of human and mouse UBA6 by western blot. Samples: Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-UBA6 antibody used for WB at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30013_rabbit-flj10808-pab-2312025128816.jpg "Western Blot:[NBP3-30013]-Detection of human and mouse UBA6 by western blot. Samples: Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-UBA6 antibody used for WB at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:UQCRQ AntibodyNBP3-30631] -Detection of human UQCRQ by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UQCRQ antibody (NBP3-30631 lot 1) used for IP at 2 µg per reaction. UQCRQ was also immunoprecipitated by rabbit anti-UQCRQ.. For blotting immunoprecipitated UQCRQ, NBP3-30631 was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30631_rabbit-uqcrq-pab-3012202412392147.jpg "Immunoprecipitation:UQCRQ AntibodyNBP3-30631] -Detection of human UQCRQ by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UQCRQ antibody (NBP3-30631 lot 1) used for IP at 2 µg per reaction. UQCRQ was also immunoprecipitated by rabbit anti-UQCRQ.. For blotting immunoprecipitated UQCRQ, NBP3-30631 was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot:[NBP3-29856]-Detection of human and mouse NEDD4L by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NEDD4L antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 3 µg/mg lysate. NEDD4L was also immunoprecipitated by rabbit anti-NEDD4L antibodies and , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 second and 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29856_rabbit-nedd4l-pab-231202511585555.jpg "Western Blot:[NBP3-29856]-Detection of human and mouse NEDD4L by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NEDD4L antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 3 µg/mg lysate. NEDD4L was also immunoprecipitated by rabbit anti-NEDD4L antibodies and , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 second and 10 seconds.")
![Immunoprecipitation:MIOS AntibodyNBP3-30541] -Detection of human MIOS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIOS antibody NBP3-30541 (lot NBP3-30541-1) used for IP at 6 µg per reaction. MIOS was also immunoprecipitated by rabbit anti-MIOS antibody BL18122. For blotting immunoprecipitated MIOS, NBP3-30541 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30541_rabbit-mios-pab-301220242033454.jpg "Immunoprecipitation:MIOS AntibodyNBP3-30541] -Detection of human MIOS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIOS antibody NBP3-30541 (lot NBP3-30541-1) used for IP at 6 µg per reaction. MIOS was also immunoprecipitated by rabbit anti-MIOS antibody BL18122. For blotting immunoprecipitated MIOS, NBP3-30541 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")