| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00319-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12711-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98923-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89198-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12540-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27179-0.025ml | |||
![Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-278202415212422.jpg "Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32649, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29820241420992.jpg)
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32649) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32649) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-298202414333659.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29202410234263.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - ytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody (DX2) Green - anti-CD95 (LT95) A - JURKAT human peripheral blood T cell leukemia cell line B - RAMOS human Burkitt lymphoma cell line C - CEM human leukemia cell line D - MOLT-4 human acute lymphoblastic T cell leukemia cell line](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0004.jpg "Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - ytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody (DX2) Green - anti-CD95 (LT95) A - JURKAT human peripheral blood T cell leukemia cell line B - RAMOS human Burkitt lymphoma cell line C - CEM human leukemia cell line D - MOLT-4 human acute lymphoblastic T cell leukemia cell line")
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fig. 1. Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody Green - anti-CD95 (LT95) A. JURKAT human peripheral blood T cell leukemia cell line B. RAMOS human Burkitt lymphoma cell line C. CEM human leukemia cell line D. MOLT-4 human acute lymphoblastic T cell leukemia cell line.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0001.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - A surface stain was performed on HeLa with Fas/TNFRSF6/CD95 Antibody (LT95) NB500-503AF647 and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0003.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Using the Allophycocyanin direct conjugate Surface staining of human peripheral blood cells with anti-CD95 (LT95) APC.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0002.jpg)
![Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human liver with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-2352025162812.png "Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human liver with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human epididymis with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-235202511444411.png)
![Immunoprecipitation: DCXR Antibody [NBP3-00319] - DCXR was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate 2 uL NBP3-00319 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00319, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-235202511354130.png)
![Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human kidney with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-23520251633500.png)
![Western Blot: DCXR Antibody [NBP3-00319] - NBP3-00319 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:27 kDa Observed band size:29 kDa](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-23520251664778.png)
![Western Blot: UQCRB Antibody [NBP3-12711] - Lane A: HeLa Whole Cell LysateLane B: 293 Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa](http://images.novusbio.com/fullsize/UQCRB-Antibody-Western-Blot-NBP3-12711-img0001.jpg "Western Blot: UQCRB Antibody [NBP3-12711] - Lane A: HeLa Whole Cell LysateLane B: 293 Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa")
![Immunohistochemistry-Paraffin: UQCRB Antibody [NBP3-12711] - Staining of human UQCRB in human liver with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/UQCRB-Antibody-Immunohistochemistry-Paraffin-NBP3-12711-img0004.jpg)
![Immunohistochemistry-Paraffin: UQCRB Antibody [NBP3-12711] - Staining of human UQCRB in human kidney with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/UQCRB-Antibody-Immunohistochemistry-Paraffin-NBP3-12711-img0002.jpg)
![Immunohistochemistry-Paraffin: UQCRB Antibody [NBP3-12711] - Staining of human UQCRB in human colon carcinoma with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/UQCRB-Antibody-Immunohistochemistry-Paraffin-NBP3-12711-img0003.jpg)
![Immunoprecipitation: UQCRB Antibody [NBP3-12711] - Lane A:0.5 mg 293 Whole Cell Lysate4 uL anti-UQCRB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-UQCRB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size :14 kDa](http://images.novusbio.com/fullsize/UQCRB-Antibody-Immunoprecipitation-NBP3-12711-img0005.jpg)
![Western Blot: Actin Antibody [NBP2-98923] - Lane A: Hela Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa](http://images.novusbio.com/fullsize/Actin-Antibody-Western-Blot-NBP2-98923-img0006.jpg "Western Blot: Actin Antibody [NBP2-98923] - Lane A: Hela Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa")
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in rat skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0005.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in human prostate with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0004.jpg)
![Western Blot: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401] - Analysis of Cytokeratin 6a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80401, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HT-29 cell lysateLane 2: Human skin tissue lysate](http://images.novusbio.com/fullsize/Cytokeratin-5-6-Antibody-A2A12-Western-Blot-NBP2-80401-img0005.jpg "Western Blot: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401] - Analysis of Cytokeratin 6a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80401, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HT-29 cell lysateLane 2: Human skin tissue lysate")
![Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401] - Analysis in human esophagus tissue using Cytokeratin 6a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, w](http://images.novusbio.com/fullsize/Cytokeratin-5-6-Antibody-A2A12-Immunohistochemistry-Paraffin-NBP2-80401-img0004.jpg)
![Flow Cytometry: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401] - Analysis of Cytokeratin 6a was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Cytokeratin-5-6-Antibody-A2A12-Flow-Cytometry-NBP2-80401-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401] - Analysis in human tonsil tissue using Cytokeratin 6a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, wash](http://images.novusbio.com/fullsize/Cytokeratin-5-6-Antibody-A2A12-Immunohistochemistry-Paraffin-NBP2-80401-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401] - Analysis in human skin tissue using Cytokeratin 6a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed](http://images.novusbio.com/fullsize/Cytokeratin-5-6-Antibody-A2A12-Immunohistochemistry-Paraffin-NBP2-80401-img0003.jpg)
![Western Blot: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Western blot analysis of Caspase-8 on different lysates with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 3: Jurkat treated with 25uM Etoposide for 16 hours cell lysate Lane 4: Jurkat treated with 1uM staurosporine for 3 hours cell lysate Lane 5: HeLa cell lysate Lane 6: HeLa treated with 1uM staurosporine for 3 hours cell lysate Lane 7: HeLa treated with 100uM Etoposide for 4 hours cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 55 kDa Observed band size: 18-55 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32109) at 1/2,000 dilution was used in antibody diluent at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32109_rabbit-caspase-8-mab-psh04-77-278202416285279.jpg "Western Blot: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Western blot analysis of Caspase-8 on different lysates with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 3: Jurkat treated with 25uM Etoposide for 16 hours cell lysate Lane 4: Jurkat treated with 1uM staurosporine for 3 hours cell lysate Lane 5: HeLa cell lysate Lane 6: HeLa treated with 1uM staurosporine for 3 hours cell lysate Lane 7: HeLa treated with 100uM Etoposide for 4 hours cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 55 kDa Observed band size: 18-55 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32109) at 1/2,000 dilution was used in antibody diluent at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32109) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32109_rabbit-caspase-8-mab-psh04-77-298202414161856.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Immunocytochemistry analysis of HeLa cells treated with or without 1μM staurosporine for 3 hours labeling Caspase-8 with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32109_rabbit-caspase-8-mab-psh04-77-29202410234224.jpg)
![Flow Cytometry: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Flow cytometric analysis of HeLa cells treated with or without 1μM staurosporine for 3 hours cells labeling Caspase-8. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32109, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32109_rabbit-caspase-8-mab-psh04-77-29202416453297.jpg)
![Western Blot: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDa Observed band size:52 kDa](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Western-Blot-NBP2-89198-img0003.jpg "Western Blot: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDa Observed band size:52 kDa")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198] - Immunochemical staining of human Cytokeratin14 in human esophageal carcinoma with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89198-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198] - Staining of human CK14 in human skin with rabbit monoclonal antibody (1:20000).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89198-img0001.jpg)
![Immunoprecipitation: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A:0.5 mg A431 Whole Cell Lysate 2 uL anti-KRT14 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody:Anti-KRT14 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique. Performed under reducing conditions. Predicted band size: 52 kDa Observed band size: 52 kDa](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Immunoprecipitation-NBP2-89198-img0002.jpg)
![Western Blot: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Western blot analysis of Cytokeratin 19 on different lysates with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-278202415242660.jpg "Western Blot: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Western blot analysis of Cytokeratin 19 on different lysates with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32247) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-298202413585528.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Immunocytochemistry analysis of MCF7 cells labeling Cytokeratin 19 with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-29202410334065.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Flow cytometric analysis of MCF7 cells labeling Cytokeratin 19. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32247, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-39202488462.jpg)
![Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Lane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Western-Blot-NBP3-12540-img0005.jpg "Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Lane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Staining of human GGCT in human corpus uteri with rabbit polyclonal antibody (1:10000).](http://images.novusbio.com/fullsize/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Immunohistochemistry-Paraffin-NBP3-12540-img0003.jpg)
![Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Staining of human GGCT in human hepatoma with rabbit polyclonal antibody (1:10000).](http://images.novusbio.com/fullsize/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Immunohistochemistry-Paraffin-NBP3-12540-img0002.jpg)
![Immunoprecipitation: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Lane A:0.5 mg 293T Whole Cell Lysate2 uL anti-GGCT rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GGCT rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa](http://images.novusbio.com/fullsize/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Immunoprecipitation-NBP3-12540-img0004.jpg)
![Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Immunochemical staining of human gamma-Glutamylcyclotransferase/CRF21/GGCT in human lung cancer with NBP3-12540 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-12540_rabbit-polyclonal-gamma-glutamylcyclotransferase-crf21-ggct-antibody-235202511494039.png)
![Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0002.jpg "Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.")
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Staining of PFA fixed HEK293 cells labelled with hamster anti-Atg9 (Left) and rabbit anti-Atg9.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Immunofluorescence-NBP2-50109-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of siRNA knockdown of human Atg9 (si) in Hela and Hek293 cells compared to siControl siRNA (siC) reveals specicity of hamster anti-Atg9 antibody.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0003.jpg)
![Immunohistochemistry: TRBC1 Antibody (5A8A10) [NBP3-27179] - Analysis of paraffin-embedded spleen tissues using TRBC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27179_mouse-trbc1-mab-5a8a10-bsa-free-104202491586.jpg%3Fv=1666706084 "Immunohistochemistry: TRBC1 Antibody (5A8A10) [NBP3-27179] - Analysis of paraffin-embedded spleen tissues using TRBC1 mouse mAb with DAB staining.")
![Western Blot: TRBC1 Antibody (5A8A10) [NBP3-27179] - Analysis using TRBC1 mAb against HEK293-6e (1) and TRBC1 (AA: 1-149)-hIgGFc transfected HEK293-6e (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27179_mouse-trbc1-mab-5a8a10-bsa-free-1042024917023.jpg%3Fv=1666706084)
![ELISA: TRBC1 Antibody (5A8A10) [NBP3-27179] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27179_mouse-trbc1-mab-5a8a10-bsa-free-1042024915814.jpg%3Fv=1666706084)
![Flow Cytometry: TRBC1 Antibody (5A8A10) [NBP3-27179] - Analysis of JURKAT cells using TRBC1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27179_mouse-trbc1-mab-5a8a10-bsa-free-10420249141920.jpg%3Fv=1666706084)
![Western Blot: TRBC1 Antibody (5A8A10) [NBP3-27179] - Analysis using TRBC1 mAb against human TRBC1 (AA: 1-149) recombinant protein. (Expected MW is 42.8 kDa)](http://images.novusbio.com/fullsize/nbp3-27179_mouse-trbc1-mab-5a8a10-bsa-free-10420249181948.jpg%3Fv=1666706084)