| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97853-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90271-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99514-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98822-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98826-50ul | |||
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)
![Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-278202416331236.jpg "Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32046) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-29820241492659.jpg)
![Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP2-97853] - Immunochemical staining of human NDUFB5 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Paraffin-NBP2-97853-img0003.jpg "Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP2-97853] - Immunochemical staining of human NDUFB5 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP2-97853] - Immunochemical staining of human NDUFB5 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Paraffin-NBP2-97853-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFB5 Antibody [NBP2-97853] - Immunofluorescence staining of NDUFB5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97853 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97853_rabbit-polyclonal-ndufb5-antibody-235202511444486.png)
![Western Blot: CD11b Antibody (PSH03-96) [NBP3-32121] - Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (NBP3-32121) at 1/2,000 dilution. Lane 1: TF-1 cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (15 ug/Lane) Lane 3: U-937 cell lysate (30 ug/Lane) Lane 4: Jurkat cell lysate (negative) (10 ug/Lane) Lane 5: RAW264.7 cell lysate (5 ug/Lane) Lane 6: J774A.1 cell lysate (5 ug/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32121_rabbit-cd11b-mab-psh03-96-278202415295518.jpg "Western Blot: CD11b Antibody (PSH03-96) [NBP3-32121] - Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (NBP3-32121) at 1/2,000 dilution. Lane 1: TF-1 cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (15 ug/Lane) Lane 3: U-937 cell lysate (30 ug/Lane) Lane 4: Jurkat cell lysate (negative) (10 ug/Lane) Lane 5: RAW264.7 cell lysate (5 ug/Lane) Lane 6: J774A.1 cell lysate (5 ug/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD11b Antibody (PSH03-96) [NBP3-32121] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-CD11b antibody (NBP3-32121) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32121) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32121_rabbit-cd11b-mab-psh03-96-29820241435252.jpg)
![Western Blot: PTH Antibody (JE66-58) [NBP3-32717] - Western blot analysis of PTH on different lysates with Rabbit anti-PTH antibody (NBP3-32717) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: CAL-62 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 13 kDa Observed band size: 16 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32717) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32717_rabbit-pth-mab-je66-58-278202416285238.jpg "Western Blot: PTH Antibody (JE66-58) [NBP3-32717] - Western blot analysis of PTH on different lysates with Rabbit anti-PTH antibody (NBP3-32717) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: CAL-62 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 13 kDa Observed band size: 16 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32717) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTH Antibody (JE66-58) [NBP3-32717] - Immunohistochemical analysis of paraffin-embedded human parathyroid tissue with Rabbit anti-PTH antibody (NBP3-32717) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32717) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32717_rabbit-pth-mab-je66-58-298202414304131.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0002.jpg "Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunofluorescence staining of AIF-1/Iba1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90271 (dilution ratio 1:60) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90271_rabbit-monoclonal-aif-1-iba1-antibody-031-235202514492357.png)
![Western Blot: SPIN1 Antibody [NBP2-83590] - WB Suggested Anti-SPIN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain](http://images.novusbio.com/fullsize/SPIN1-Antibody-Western-Blot-NBP2-83590-img0003.jpg "Western Blot: SPIN1 Antibody [NBP2-83590] - WB Suggested Anti-SPIN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain")
![Immunohistochemistry-Paraffin: SPIN1 Antibody [NBP2-83590] - Rabbit Anti-SPIN1 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Thyroid. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x. Exposure Time: 0.5-2.0sec](http://images.novusbio.com/fullsize/SPIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-83590-img0002.jpg)
![Immunohistochemistry-Paraffin: SPIN1 Antibody [NBP2-83590] - Rabbit Anti-SPIN1 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Testis. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x. Exposure Time: 0.5-2.0sec](http://images.novusbio.com/fullsize/SPIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-83590-img0001.jpg)
![Western Blot: CYBB/NOX2 Antibody (SN07-16) [NBP2-67680] Analysis of NOX2/gp91phox on different lysates with Rabbit anti-NOX2/gp91phox antibody at 1/2,000 dilution.Lane 1: HepG2 cell lysate (15 ug/Lane)Lane 2: MCF7 cell lysate (15 ug/Lane)Lane 3: Mouse brain tissue lysate (20 ug/Lane)Lane 4: Mouse spleen tissue lysate (20 ug/Lane)Lane 5: Rat brain tissue lysate (20 ug/Lane)Lane 6: Rat spleen tissue lysate (20 ug/Lane)Predicted band size: 65 kDaObserved band size: 55 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67680_rabbit-monoclonal-cybb-nox2-antibody-sn07-16-western-blot-197202491539..jpg "Western Blot: CYBB/NOX2 Antibody (SN07-16) [NBP2-67680] Analysis of NOX2/gp91phox on different lysates with Rabbit anti-NOX2/gp91phox antibody at 1/2,000 dilution.Lane 1: HepG2 cell lysate (15 ug/Lane)Lane 2: MCF7 cell lysate (15 ug/Lane)Lane 3: Mouse brain tissue lysate (20 ug/Lane)Lane 4: Mouse spleen tissue lysate (20 ug/Lane)Lane 5: Rat brain tissue lysate (20 ug/Lane)Lane 6: Rat spleen tissue lysate (20 ug/Lane)Predicted band size: 65 kDaObserved band size: 55 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: S100P Antibody [NBP2-99514] - Immunochemical staining of human S100P in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/S100P-Antibody-Immunohistochemistry-Paraffin-NBP2-99514-img0003.jpg "Immunohistochemistry-Paraffin: S100P Antibody [NBP2-99514] - Immunochemical staining of human S100P in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: S100P Antibody [NBP2-99514] - Immunochemical staining of human S100P in human pancreatic carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/S100P-Antibody-Immunohistochemistry-Paraffin-NBP2-99514-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S100P Antibody [NBP2-99514] - Immunofluorescence staining of S100P in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99514 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-99514_rabbit-polyclonal-s100p-antibody-235202511254422.png)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human lymph node with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-98822-img0003.jpg "Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human lymph node with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-98822-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-98822] - Immunofluorescence staining of PML Protein in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98822 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat.](http://images.novusbio.com/fullsize/nbp2-98822_rabbit-polyclonal-pml-protein-antibody-235202521451777.png)
![Western Blot: TLK1 Antibody [NBP2-98826] - Lane A: 293T Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:87 kDaObserved band size:87 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/TLK1-Antibody-Western-Blot-NBP2-98826-img0003.jpg "Western Blot: TLK1 Antibody [NBP2-98826] - Lane A: 293T Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:87 kDaObserved band size:87 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: TLK1 Antibody [NBP2-98826] - Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate4 uL anti-TLK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TLK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 87 kDaObserved band size: 87 kDa](http://images.novusbio.com/fullsize/TLK1-Antibody-Immunoprecipitation-NBP2-98826-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TLK1 Antibody [NBP2-98826] - Immunofluorescence staining of TLK1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98826 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98826_rabbit-polyclonal-tlk1-antibody-235202511354115.png)
![Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-288202412442957.jpg "Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Immunocytochemistry analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes, then treated with lambda pp for 1 hour labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-29202410234248.jpg)