| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56359SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44730 | |||
| Azide and BSA Free | NBP2-34767-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47835-0.1mg | |||
| IHC-Prediluted | NBP2-48284 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36748 | |||
| BSA Free | NBP2-36748-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87693-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84697-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26439-50ul | |||
![Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NB100-56359-img0005.jpg "Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.")
![Immunohistochemistry-Paraffin: APE Antibody [NB100-56359] - Human Breast: FFPE tissue section using APE antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-Paraffin-NB100-56359-img0004.jpg)
![Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2682024158269.jpg "Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL")
![Immunohistochemistry: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-PCNA antibody (NBP3-32725) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32725) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2982024149267.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunocytochemistry analysis of HeLa cells labeling PCNA with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-29202410484872.jpg)
![Western Blot: PCNA Antibody (A6-G11-R) [NBP3-32725] - Western blot analysis of PCNA on different lysates with Mouse anti-PCNA antibody (NBP3-32725) at 1/20,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: HCT 116 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: L6 cell lysate (15 µg/Lane) Lane 8: Mouse spleen tissue lysate (15 µg/Lane) Lane 9: Rat spleen tissue lysate (15 µg/Lane) Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 11 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-3920248485013.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989] - Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Antibody (SPM350).](http://images.novusbio.com/fullsize/PCNA-Antibody-SPM350-Immunohistochemistry-Paraffin-NBP2-32989-img0002.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989] - Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Antibody (SPM350).")
![Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Western-Blot-NBP2-44731-img0002.jpg "Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)")
![Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) [NBP2-44731] - Human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Immunohistochemistry-Paraffin-NBP2-44731-img0001.jpg)
![Flow Cytometry: PCNA Antibody (PCNA/694) [NBP2-44731] - Flow Cytometric Analysis of PFA-fixed HeLa cells using PCNA Antibody (PCNA/694) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Flow-Cytometry-NBP2-44731-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMUG1 Antibody [NB100-1414] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/SMUG1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1414-img0002.jpg "Immunocytochemistry/Immunofluorescence: SMUG1 Antibody [NB100-1414] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: SMUG1 Antibody [NB100-1414] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/SMUG1-Antibody-Flow-Cytometry-NB100-1414-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMUG1 Antibody [NB100-1414] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/SMUG1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1414-img0001.jpg)
![Lane 1: Marker [kDa]Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp3-44462_mouse-parp-mab-cl2209-azide-and-bsa-free-28220251217311.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody [NBP1-87693] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87693-img0004.jpg "Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody [NBP1-87693] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NBP1-87693] -Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87693_rabbit-polyclonal-kat3b-p300-antibody-immunohistochemistry-paraffin-2132024101550..jpg)
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NBP1-87693] -Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/antibody/nbp1-87693_rabbit-polyclonal-kat3b-p300-antibody-immunohistochemistry-paraffin-2132024101644..jpg)
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NBP1-87693] -Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87693_rabbit-polyclonal-kat3b-p300-antibody-immunohistochemistry-paraffin-2132024101812..jpg)
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NBP1-87693] -Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-87693_rabbit-polyclonal-kat3b-p300-antibody-immunohistochemistry-paraffin-2132024102050..jpg)
![Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697] - Staining in human lymph node and kidney tissues using anti-FEN1 antibody. Corresponding FEN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84697-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697] - Staining in human lymph node and kidney tissues using anti-FEN1 antibody. Corresponding FEN1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84697-img0007.jpg)
![Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84697-img0008.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-84697_rabbit-polyclonal-fen-1-antibody-104202514515335.jpg)
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-262202415475644.jpg "Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-2722024850467.jpg)
![Western Blot: PARP Antibody (8C7) [NBP3-26439] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 KDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-282202411485824.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (8C7) [NBP3-26439] - Staining of Hela Cells with PARP Antibody (8C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241323611.jpg)
![Flow Cytometry: PARP Antibody (8C7) [NBP3-26439] - Overlay histogram showing Jurkat cells stained with PARP Antibody (8C7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241431270.jpg)
![Western Blot: XRCC1 Antibody [H00007515-B01P] - Analysis of XRCC1 expression in transfected 293T cell line by XRCC1 polyclonal antibody. Lane 1: XRCC1 transfected lysate(69.63 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/XRCC1-Antibody-Western-Blot-H00007515-B01P-img0006.jpg "Western Blot: XRCC1 Antibody [H00007515-B01P] - Analysis of XRCC1 expression in transfected 293T cell line by XRCC1 polyclonal antibody. Lane 1: XRCC1 transfected lysate(69.63 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: XRCC1 Antibody [H00007515-B01P] - Analysis of purified antibody to XRCC1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/XRCC1-Antibody-Immunocytochemistry-Immunofluorescence-H00007515-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: XRCC1 Antibody [H00007515-B01P] - Analysis of purified antibody to XRCC1 on formalin-fixed paraffin-embedded human kidney. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/XRCC1-Antibody-Immunohistochemistry-Paraffin-H00007515-B01P-img0005.jpg)
![Simple Western: TDG Antibody [NBP2-20627] - Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TDG-Antibody-Western-Blot-NBP2-20627-img0006.jpg "<b>Genetic Strategies Validation. </b>Simple Western: TDG Antibody [NBP2-20627] - Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TDG Antibody [NBP2-20627] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TDG protein stained by TDG antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/TDG-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20627-img0005.jpg)
![Western Blot: TDG Antibody [NBP2-20627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (NBP2-20627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20627_rabbit-polyclonal-tdg-antibody-9102024206333.jpg)
![Western Blot: TDG Antibody [NBP2-20627] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (NBP2-20627) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-20627_rabbit-polyclonal-tdg-antibody-910202420122027.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TDG Antibody [NBP2-20627] - Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20627_rabbit-polyclonal-tdg-antibody-1610202419414545.jpg)
![Western Blot: TDG Antibody [NBP2-32248] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Thymine DNA glycosylase antibody NBP2-32248 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TDG-Antibody-Western-Blot-NBP2-32248-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TDG Antibody [NBP2-32248] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Thymine DNA glycosylase antibody NBP2-32248 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TDG Antibody [NBP2-32248] - FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- TDG used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TDG-Antibody-Immunohistochemistry-NBP2-32248-img0004.jpg)
![Immunohistochemistry-Paraffin: TDG Antibody [NBP2-32248] - FFPE section of human mammary glands. Antibody: Affinity purified rabbit anti- TDG used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TDG-Antibody-Immunohistochemistry-NBP2-32248-img0003.jpg)
![Immunoprecipitation: TDG Antibody [NBP2-32248] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-Thymine DNA glycosylase antibody NBP2-32248 used for IP at 6 ug per reaction. Thymine DNA glycosylase was also immunoprecipitated by rabbit anti-Thymine DNA glycosylase antibody BL16331. For blotting immunoprecipitated Thymine DNA glycosylase, NBP2-32248 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TDG-Antibody-Immunoprecipitation-NBP2-32248-img0002.jpg)