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![SDS-Page: Recombinant Human MEK1 Protein [H00005604-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005604-Q01-1.jpg "SDS-Page: Recombinant Human MEK1 Protein [H00005604-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: ATG4A Recombinant Protein [H00115201-P01]](http://images.novusbio.com/fullsize/ATG4A-Recombinant-Protein-SDS-Page-H00115201-P01-img0001.jpg "SDS-Page: ATG4A Recombinant Protein [H00115201-P01]")
![Western Blot: ATG4B Antibody (JE59-97) [NBP3-32057] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32057) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32057) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32057_rabbit-atg4b-mab-je59-97-278202416285266.jpg "Western Blot: ATG4B Antibody (JE59-97) [NBP3-32057] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32057) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32057) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32790_rabbit-pras40-p-thr246-mab-je53-94-288202412442948.jpg "Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: AKT1 [p Ser129] Antibody (JE59-50) [NBP3-32748] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32748) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32748) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32748_rabbit-akt1-p-ser129-mab-je59-50-27820241631645.jpg "Western Blot: AKT1 [p Ser129] Antibody (JE59-50) [NBP3-32748] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32748) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32748) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32813_rabbit-tsc2-p-thr1462-mab-je43-81-288202412461624.jpg "Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Protein Array: Elk-1 Antibody (PCRP-ELK1-1B9) [NBP3-08885] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Elk-1 Mouse Monoclonal Antibody (PCRP-Elk-1-1B9).](http://images.novusbio.com/fullsize/Elk-1-Antibody-PCRP-ELK1-1B9-Protein-Array-NBP3-08885-img0001.jpg "Protein Array: Elk-1 Antibody (PCRP-ELK1-1B9) [NBP3-08885] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Elk-1 Mouse Monoclonal Antibody (PCRP-Elk-1-1B9).")
