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Nitrosylation Bioinformatics

Nitrosylation is a term used to describe the post-translational modification (PTM) in which nitric oxide is covalently incorporated into thiol groups of free cystine residues to form S-nitrothiols (SNOs). The reverse of this reaction is called denitrosylation. In order to prevent denitrosylation, SNOs are stored in vesicles and membranes. In response to various cellular signals, SNOs are released into the cytoplasm to be denitrosylated.

Nitrosylation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Nitrosylation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1495 products for the study of Nitrosylation that can be applied to Chromatin Immunoprecipitation, Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP) from our catalog of antibodies and ELISA kits.

NB300-605
Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours (right lane) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     2 Reviews

28 Publications
NB100-858
Western Blot: nNOS Antibody [NB100-858] - Staining of Human Frontal Cortex (A) and (1ug/ml) Brain Sub Ventricular Zone (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear and Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

     1 Review

8 Publications
NB300-500
Immunohistochemistry: eNOS Antibody [NB300-500] - The pulmonary tissues were analyzed for expression of eNOS protein by immunohistochemistry(IHC). Dark brown staining of indicates eNOS-positive cells. IHC stain of representative pulmonary artery endothelial cells from rats subjected to CON=Control Group, ALI=Acute lung injury Group, LV=Low tidal volume Group, VLV=Very low tidal volume Group, MV=Large tidal volume Group (magnification, x400).Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-Fr

     3 Reviews

11 Publications
NBP2-47415
Western Blot: ISYNA1 Antibody [NBP2-47415] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10<br/>Lane 2: Human cell line HEK 293Immunocytochemistry/Immunofluorescence: ISYNA1 Antibody [NBP2-47415] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & cytosol.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NB300-221
Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Image from verified customer review.Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH, NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     16 Reviews

246 Publications
NB100-56708
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3.Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Western Blot Image of anti-Caspase 3 (31A1067).  Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST.  The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent.  Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, EM

     1 Review

115 Publications
NBP1-88769
Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in human cell lines U2OS and MCF-7 using anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining in human cerebral cortex and pancreas tissues using anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

4 Publications
NBP1-76917
Western Blot: Nanos2 Antibody [NBP1-76917] - Rat brain tissue lysate with Nanos2 antibody at (A) 1 and (B) 2 ug/mL.Immunocytochemistry/Immunofluorescence: Nanos2 Antibody [NBP1-76917] - Immunofluorescence of Nanos2 in Human Testis cells with Nanos2 antibody at 20 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

210-TA
Recombinant Human TNF-alpha  (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.


Species Human

     21 Reviews

520 Publications
NBP2-52983
Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

NB600-41532
Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.

Goat Polyclonal
Species Mouse
Applications WB, ELISA, ICC/IF

39 Publications
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog #<A class=NoLineLink href=Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

19 Publications
NB100-56583
Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, Flow, IHC-P

     1 Review

1 Publication
NBP1-77306
Western Blot: SnoN Antibody [NBP1-77306] - Analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 ug/mL.Immunohistochemistry-Paraffin: SnoN Antibody [NBP1-77306] - Mouse lung tissue with SnoN antibody at 5 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IHC

     1 Review

NBP1-83837
Western Blot: SBNO1 Antibody [NBP1-83837] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)<br/>Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Immunocytochemistry/Immunofluorescence: SBNO1 Antibody [NBP1-83837] - Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-76919
Western Blot: Nanos3 Antibody [NBP1-76919] - Human brain tissue lysate with Nanos3 antibody at 2 ug/mL.Immunocytochemistry/Immunofluorescence: Nanos3 Antibody [NBP1-76919] - in Human Brain cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF