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![Western Blot: RFP Antibody [NBP1-69962] - Western blot of RFP recombinant protein detected with RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0025.jpg "Western Blot: RFP Antibody [NBP1-69962] - Western blot of RFP recombinant protein detected with RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Immunofluorescence Microscopy of RFP antibody. Tissue: (10X) Mouse E14.5 embryo heart tissue.Fixation: 4% PFA. Antigen retrieval: Heat.Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT.Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT.Staining: cardiac cells are RFP positive in red in tomato transgenic mice.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0019.jpg)
![Immunohistochemistry: RFP Antibody [NBP1-69962] - Immunohistochemistry of RFP antibody.Tissue: Mouse gut tissue in tomato transgenic mice.Fixation: formalin fixed paraffin embedded. Antigen retrieval: heat 5 min at high temp in 1X rodent decloaker. Primary antibody: RFP antibody at 1:200 for 1 h 30 min at RT.Secondary Antibody: HRP anti-rabbit.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunohistochemistry-NBP1-69962-img0026.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye 800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye 800 fluorescence image was captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0008.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: RFP. Lane 2: Human IgG. Lane 3: Goat IgG. Lane 4: Mouse IgG. Load: 50ng per lane.Primary antibody: RFP antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase conjugated rabbit secondary antibody at 1:40,000 for 30 min at RT.Block Blocking Buffer for 30 min at RT.Predicted/Observed size: 27 kDa, 30 kDa.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0021.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: Opal Prestained Marker.Lane 2: 50ng of RFP. Primary Antibody: Anti-RFP at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70,000 for 30mins at RT. Block: BlockOut Universal blocking buffer. Expect ~27kDa.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0022.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: CHO/K1 Lysate. Lane 4: MDA-MB-231. Lane 5: A431 Lysate. Lane 6: Jurkat Lysate. Lane 7: NIH/3T3 Lysate. Lane 8: E-coli HCP Control. Lane 9: FLAG Positive Control Lysate. Lane 10: Red Fluorescent Protein. Lane 11: Green Fluorescent Protein. Lane 12: Glutathione-S-Transferase Protein. Lane 13: Maltose Binding Protein. Load: 10 ug of lysate or 50ng of purified protein per lane. Primary antibody: RFP antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 30 kDa for RFP.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0023.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: RFP/HEK293T WCL [0.05/10ug] [+]. Lane 3: HEK293T Whole Cell Lysate [-]. Lane 4: RFP/NIH/3T3 Whole Cell Lysate [0.05/10ug] [+]. Lane 5: NIH/3T3 Whole Cell Lysate [-]. Lane 6: RFP/PC-12 Whole Cell Lysate [0.05/10ug] [+]. Lane 7: PC-12 Whole Cell Lysate [-]. Primary Antibody: Anti-RFP at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase at 1:70,000 at RT for 30 mins. Predicted/Observed MW: ~27kDa. Exposure: 2 sec.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: HopERCre/+; R26Tom/+ mice. Fixation: 0.5% PFA. Antigen retrieval: Tamoxifen. Primary antibody: RFP antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: Hop-derived cells in the hair follicle, labeled in red.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: (10X) Mouse lung tissue. Fixation: 4% PFA. Antigen retrieval: Heat. Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT. Staining: SPC+ cells are RFP positive in red.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: (10X) Mouse E14.5 embryo heart tissue. Fixation: 4% PFA. Antigen retrieval: Heat. Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT. Staining: cardiac cells are RFP positive in red in tomato transgenic mice.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0003.jpg)
![CyTOF-ready: RFP Antibody [NBP1-69962] - Analysis of RFP antibody on human tissue. RFP-expressing cells (red), nuclei (blue). Scale, 50 um. Image from verified customer review](http://images.novusbio.com/fullsize/RFP-Antibody-CyTOF-ready-NBP1-69962-img0016.jpg)
![ELISA: RFP Antibody [NBP1-69962] - ELISA Results of RFP antibody tested against purified RFP protein. Each well was coated in duplicate with 1.0 ug of RFP [red], Human IgG [green], Mouse IgG [blue], Rat IgG [purple]. The starting dilution of antibody was 5ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 1% Fish Gel in PBS Blocking Buffer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate. Results show RFP titer >75,000, Human, Mouse, Rat IgG titers <100.](http://images.novusbio.com/fullsize/RFP-Antibody-ELISA-NBP1-69962-img0027.jpg)
