Zymosan, TLR2 Ligand

Images

 
Zymosan, TLR2 Ligand [NBP2-26233] - Ligand activity of Zymosan. The NF-kB/SEAPorter/HEK 293 and TLR2/NFkB/SEAPorter/ HEK 293 cell line cell lines were plated in a 96-well plate at 5 x 10^4 cells/well for 16 h. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, In vitro, In vivo, LA

Order Details

Zymosan, TLR2 Ligand Summary

Description
Zymosan is a yeast cell wall derivative that is composed of b-glucans, mannans, mannoproteins and chitin. Zymosan is a known activator of TLR2, of which inflammatory responses to this particle are synergistically generated by collaboration with Dectin-1 and result in NF-kB-induced inflammatory cytokine production and phagocytosis1. Zymosan is also known to directly bind TLR2. Product Validation The data shows that Zymosan activated the TLR2 cells but not the control cells.

Applications/Dilutions

Application Notes
This product is useful for Activation of Toll-like Receptor 2 (TLR2).Stimulation of TLR2 has been reported with 1-10 ug/ml. Note: TLR2 activation at this range of concentrations of Zymosan has also been observed in our product validation tests, please see the image. Use in In vivo assay and Functional inhibition reported in scientific literature (PMID 25846753). Use in Ligand activation reported in scientific literature (PMID 25957979). Use in In vitro reported in scientific literature (PMID 27351455).
Publications
Read Publications using
NBP2-26233 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25957979)

Packaging, Storage & Formulations

Storage
Store at -20C in powder form. Store at -80C once reconstituted.
Reconstitution Instructions
To prepare a 50mg/ml stock solution, mix 50mg Zymosan with 1ml ethanol in a sterile non-pyrogenic vial by vortexing. For further dilution, use sterilized endotoxin-free water or PBS. The stock solution is insoluble and remains opaque. Vortex before use.

Alternate Names for Zymosan, TLR2 Ligand

  • Zymosan, TLR2 Ligand

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for Zymosan, TLR2 Ligand (NBP2-26233)(5)

We have publications tested in 2 confirmed species: Human, Bacteria.

We have publications tested in 4 applications: Func, In vitro, In-vivo, LA.


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Func
(3)
In vitro
(2)
In-vivo
(1)
LA
(1)
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Human
(3)
Bacteria
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Showing Publications 1 - 5 of 5.
Publications using NBP2-26233 Applications Species
Hikita, N;Cho, Y;Tachibana, D;Hamazaki, T;Koyama, M;Tokuhara, D; Cell surface antigens of neonatal monocytes are selectively impaired in basal expression, but hyperresponsive to lipopolysaccharide and zymosan Journal of Reproductive Immunology Sep 1 2019 [PMID: 31606697] (Func, Human) Func Human
Yamaguchi M, Hirose Y, Takemura M, et al Streptococcus pneumoniae Evades Host Cell Phagocytosis and Limits Host Mortality Through Its Cell Wall Anchoring Protein PfbA. Front Cell Infect Microbiol. 2019 Aug 20 [PMID: 31482074] (Bacteria)

Details:
Mice were infected with S. pneumoniae.
Bacteria
Yanai S, Tokuhara D, Tachibana D et al. Diabetic pregnancy activates the innate immune response through TLR5 or TLR1/2 on neonatal monocyte J. Reprod. Immunol. 2016 Jun 22 [PMID: 27351455] (In vitro, Human) In vitro Human
Nohmi K, Tokuhara D, Tachibana D et al. Zymosan Induces Immune Responses Comparable with Those of Adults in Monocytes, Dendritic Cells, and Monocyte-Derived Dendritic Cells from Cord Blood. J. Pediatr. 2015 May 06 [PMID: 25957979] (LA, Func, In vitro, Human)

Details:
Zymosan, TLR2 ligand (Imgenex IMG-2212) was used for in-vitro stimulation experiments involving human heparinized cord or adult blood Monocytes, peripheral blood dendritic cells (DCs) and monocyte-derived DCs (MoDCs). Zymosan was employed at 1 ug/mL concentration on Monocytes as well as on MoDCs and at 0.5 ug/mL on DCs. See full text for experimental details and results.
LA, Func, In vitro Human
Farnebo L, Shahangian A, Lee Y et al. Targeting Toll-like receptor 2 inhibits growth of head and neck squamous cell carcinoma Oncotarget 2015 Apr 02 [PMID: 25846753] (Func, In-vivo) Func, In-vivo

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FAQs for Zymosan, TLR2 Ligand (NBP2-26233). (Showing 1 - 1 of 1 FAQs).

  1. What is the aproximate molecular weight of the zymosan TLR2 Ligand, NBP2-26233?
    • Since Zymosan is a polymer and therefore can be variable (a range of molecular weights) based upon the number of subunits in the polymer. The base unit is about 15 kDa with 3 subunits A/B/C.

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