Zymosan, TLR2 Ligand [NBP2-26233] - Ligand activity of Zymosan. The NF-kB/SEAPorter/HEK 293 and TLR2/NFkB/SEAPorter/ HEK 293 cell line cell lines were plated in a 96-well plate at 5 x 10^4 cells/well for 16 h. Cells ...read more
Zymosan is a yeast cell wall derivative that is composed of b-glucans, mannans, mannoproteins and chitin. Zymosan is a known activator of TLR2, of which inflammatory responses to this particle are synergistically generated by collaboration with Dectin-1 and result in NF-kB-induced inflammatory cytokine production and phagocytosis1. Zymosan is also known to directly bind TLR2. Product Validation The data shows that Zymosan activated the TLR2 cells but not the control cells.
This product is useful for Activation of Toll-like Receptor 2 (TLR2).Stimulation of TLR2 has been reported with 1-10 ug/ml. Note: TLR2 activation at this range of concentrations of Zymosan has also been observed in our product validation tests, please see the image. Use in In vivo assay and Functional inhibition reported in scientific literature (PMID 25846753). Use in Ligand activation reported in scientific literature (PMID 25957979). Use in In vitro reported in scientific literature (PMID 27351455).
Read Publications using NBP2-26233 in the following applications:
Human reactivity reported in scientific literature (PMID: 25957979)
Packaging, Storage & Formulations
Store at -20C in powder form. Store at -80C once reconstituted.
The Zymosan suspension solution is insoluble and remains opaque. Resuspend the solution by vortexing right before use.
Reconstitute with 50 mg Zymosan with 1 ml ethanol in a sterilized non-pyrogenic vial by vortexing to a final concentration of 50 mg/ml. When diluting further, use sterilized endotoxin-free water or PBS
Alternate Names for Zymosan, TLR2 Ligand
Zymosan, TLR2 Ligand
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Publications for Zymosan, TLR2 Ligand (NBP2-26233)(3)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 4 applications: Func, In vitro, In-vivo, LA.