Recombinant Bacteria Zinc metalloprotease-1 His Protein

• Reactivity: Ba
• Applications: Enzyme Activity

Recombinant Bacteria Zinc metalloprotease-1 His Protein Summary

• Description: Full-length Zmp1 (Mycobacterium tuberculosis) fused at the N-terminus to a His-tag.
  
  Source: E. coli

  Uniprot ID: O53649

• Details of Functionality: ~0.5U/mg protein Active towards the generic fluorogenic substrate for matrix metalloproteinases MMP2/MMP7
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >98%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 73.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

M. tuberculosis

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM TRIS-HCl, pH 8.0
• Preservative: No Preservative
• Purity: >98%, by SDS-PAGE

Alternate Names for Recombinant Bacteria Zinc metalloprotease-1 His Protein

• Endopeptidase, peptidase family M13
• Zmp1

Background

M13 endopeptidases are single-pass, transmembrane zinc-metallopeptidases responsible in human for the regulation of the biological activity of many hormones and peptides and are involved in many important processes such as blood pressure regulation (Neprilysin or NEP1), cardiovascular development (Endothelin Converting Enzyme-1 or ECE-12), prevention of haemolytic reaction (KELL) and phosphate homeostasis (PHEX). Zmp1 is a soluble enzyme member of the M13 endopeptidases family that plays a key role in Mycobacterium tuberculosis pathogenicity with a still unknown mechanism. Recently, it was shown to suppress inflammasome activation by inhibiting caspase-1 activation, thus preventing processing of pro-IL-1 beta into IL-1 beta and the consequent phagosome maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O53649(Bacteria)