Reactivity | V-ViSpecies Glossary |
Applications | PAGE |
Description | Recombinant Zika Virus serotype 1 virus-like particle recombinant antigen consisting of Envelope, pre-Membrane and Membrane protein (NCBI accession #: ALX35659.1, aa 123-819). Protein contains a C-terminal His-tag Storage: Thaw quickly using a 37C water bath immediately before use. Produce desired aliquot sizes and snap-freeze in liquid nitrogen for storage at -80C. While handling product avoid excessive mixing or shocking to prevent aggregation. To mix product gently pipet up and down. Short and long term storage above -80C may result in aggregate formation. Concentration: 0.1-0.5mg/ml |
Preparation Method |
The Zika Virus VLP is produced in human cell lines using state-of-the-art expression techniques. The Zika Virus VLP is composed of partially mature particles containing Envelope (E), pre-Membrane (prM) and Membrane (M), and has been constructed purely from Zika virus sequences. Concentration and purification is then performed by a series of ultracentrifuge and chromatographical methods which result in VLPs of exceptional quality and purity. |
Source | HEK293 |
Protein/Peptide Type | Recombinant Protein |
Purity | >95% pure by SDS-PAGE |
Storage | Store at -80C. Avoid freeze-thaw cycles. |
Buffer | DPBS pH 7.4, 20% Sucrose. May contain traces of tartrate |
Preservative | No Preservative |
Purity | >95% pure by SDS-PAGE |
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