![ELISA: Zika Virus NS1 Antibody (B4) - (Uganda/Suriname), Chimeric [NBP3-14840] - An ELISA plate was coated with 2ug/ml of NS1 antigen per well, then blocked with 5% BSA. Primary antibody was titrated as shown in the figure below, starting from a concentration of 1ug/ml, and the detection antibody used was Goat anti-human IgM:HRP (1:8000). The substrate used was TMB (KPL).](http://images.novusbio.com/fullsize2/Zika-Virus-NS1-Antibody-(B4)---(Uganda-Suriname)--Chimeric-ELISA-NBP3-14840-img0001.jpg "ELISA: Zika Virus NS1 Antibody (B4) - (Uganda/Suriname), Chimeric [NBP3-14840] - An ELISA plate was coated with 2ug/ml of NS1 antigen per well, then blocked with 5% BSA. Primary antibody was titrated as shown in the figure below, starting from a concentration of 1ug/ml, and the detection antibody used was Goat anti-human IgM:HRP (1:8000). The substrate used was TMB (KPL).")
| Reactivity | V-ViSpecies Glossary |
| Applications | ELISA |
| Clone | B4 |
| Clonality | Monoclonal |
| Host | Human |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Full length recombinant NS1 protein of Zika virus produced in HEK293 cells |
| Specificity | This monoclonal antibody is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus and Yellow Fever Virus. A small amount of cross-reactivity has been observed with the NS1 protein from West Nile Virus in a direct ELISA. |
| Isotype | IgM |
| Clonality | Monoclonal |
| Host | Human |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The antibody is designed to provide a control for assays in which human serum is tested for antibodies specific for Zika NS1 protein, which will result from the immune response following an infection with Zika virus. The antibody has been tested in ELISA using recombinant Zika NS1 protein as the target antigen. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS pH 7.4 |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: Zika Virus NS1 Antibody (B4) - (Uganda/Suriname), Chimeric [NBP3-14840] - An ELISA plate was coated with 10ng and 100ng of NS1 antigen per well, then blocked with 1% BSA. Primary antibody was used at a concentration of 1ug/ml, and the detection antibody used was Goat anti-mouse IgG:HRP (1:2000). The substrate used was TMB (KPL). The assay confirmed high specificity for Zika virus NS1; there was no cross-reactivity with NS1 from Dengue virus serotypes 1-4 (DENV), West Nile virus (WNV), Yellow Fever virus (YFV), Japanese Encephalitis virus (JEV) and Tick-borne Encephalitis virus (TBE).](http://images.novusbio.com/fullsize2/Zika-Virus-NS1-Antibody-(B4)---(Uganda-Suriname)--Chimeric-ELISA-NBP3-14840-img0002.jpg "ELISA: Zika Virus NS1 Antibody (B4) - (Uganda/Suriname), Chimeric [NBP3-14840] - An ELISA plate was coated with 10ng and 100ng of NS1 antigen per well, then blocked with 1% BSA. Primary antibody was used at a concentration of 1ug/ml, and the detection antibody used was Goat anti-mouse IgG:HRP (1:2000). The substrate used was TMB (KPL). The assay confirmed high specificity for Zika virus NS1; there was no cross-reactivity with NS1 from Dengue virus serotypes 1-4 (DENV), West Nile virus (WNV), Yellow Fever virus (YFV), Japanese Encephalitis virus (JEV) and Tick-borne Encephalitis virus (TBE).")
![Western Blot: Goat anti-Human IgG Fc Secondary Antibody [NB7446] - Image from verified customer review. Image using the HRP form of this antibody.](http://images.novusbio.com/images2/Goat-anti-Human-IgG-Fc-Secondary-Antibody-Western-Blot-NB7446-img0001.jpg "Western Blot: Goat anti-Human IgG Fc Secondary Antibody [NB7446] - Image from verified customer review. Image using the HRP form of this antibody.")