Zika virus (H/PF/2013) NS1 Antibody (990948) [Alexa Fluor® 405]

• Reactivity: V
• Applications: ELISA
• Clone: 990948
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Zika virus (H/PF/2013) NS1 Antibody (990948) [Alexa Fluor® 405] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived recombinant Viral Zika Virus (H/PF/2013) NS1
  Val796 & Ser799-Ser1148, with a C-terminal 6-His tag
  Accession # AHZ13508
• Specificity: Detects Viral Zika Virus (H/PF/2013) NS1 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Zika virus (H/PF/2013) NS1 Antibody (990948) [Alexa Fluor® 405]

• Zika Virus (H/PF/2013) NS1

Background

Zika Virus non-structural protein 1 (ZIKV NS1) is an approximately 48 kDa viral glycoprotein (1). Zika Virus is a mosquito-borne flavivirus and has been implicated an association with neonatal microcephaly and neurological disorders such as Guillain-Barré syndrome (2). ZIKV strain H/PF/2013 was isolated from human host.  ZIKV NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (1, 2). Infected cells secrete NS1 as a lipoprotein, which is involved in immune evasion and pathogenesis via interaction with components of the innate and adaptive immune systems (1, 2). Mature ZIKV NS1 contains 352 amino acids (aa) and has a hexameric conformation consisting of three dimers to form a symmetric barrel shape (1). It has high structural similarity to other flavivirus NS1 proteins, such as DENV and WNV (2, 3). Mature NS1 of ZIKV strain H/PF/2013 shares 98% aa sequence identity with NS1 of ZIKV strain MR 766 that was isolated from monkey host.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • AHZ13508()