ZEARALENONE ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. Zearalenone ELISA Kit is a competitive binding enzyme immunoassay for the quantitative measurement of zearalenone. |
| Sensitivity |
The actual detection limit is based on the setup of experiment protocol, such as extraction procedure and dilution factor. |
| Assay Type |
Competitive ELISA |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- 96-well microtiter plate (#S). Twelve strips of 8 detachable wells, coated with AntiZearalenone antibody.
- Calibrators containing 0, 1, 3 and 10 ng/mL of zearalenone.
- p-Nitrophenyl Phosphate (pNPP) substrate (#5). Ready to use.
- Stop Solution, 3 N NaOH (#7).
- Wash Buffer (10x PBS-Tween) (#6). Dilute 10 fold with distilled or deionized water to150 mL prior to use.
- Zearalenone-Alkaline phosphatase conjugate (ZLN-ALP) (#3).
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Background
Zeralenone is a mycotoxin affecting cereal grains for human and aninal consumption.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for ZEARALENONE ELISA Kit (KA1428). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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